| Project/Area Number |
18K15802
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 53010:Gastroenterology-related
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| Research Institution | Tohoku University |
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Principal Investigator |
Iwata Tomoaki 東北大学, 医学系研究科, 非常勤講師 (30803647)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 肝細胞癌 / 腫瘍免疫 / non coding RNA / 抑制系免疫細胞 / cell-free DNA / TERT promoter / SNHG8 / MDSC / TAM / SNHG5 / SNHG6 / non-coding RNA |
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| Outline of Final Research Achievements |
We examined the expression of non-coding RNA in human HCC using the database at “https://portal.gdc.cancer.gov/”. SNHG5, SNHG6 and SNHG8 were highly expressed in human HCC. Primers for the above RNAs were prepared, and it was confirmed that the above RNAs were highly expressed in typical HCC cell line (HepG2, Hep3B, Huh7S, Li7).
HCC cell lines in which the expression of SNHG 5, 6 and 8 was suppressed were prepared by using siRNA.
By co-culturing with the cell lines and peripheral blood lymphocytes and then performing flow cytometry, it was found that the proportion of certain suppressive immune cells was changed.
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| Academic Significance and Societal Importance of the Research Achievements |
肝細胞癌の再発や転移のリスクや予後を評価する上で、「癌細胞側の因子」 と「腫瘍免疫側の因子」を検討する必要がある。「癌細胞側の因子」としてnon- coding RNAの重要性が示され、一方で「腫瘍免疫側の因子」として、単球・マクロファージや骨髄球系細胞といった抑制系免疫細胞の重要性が示されてきているが、「癌細胞側の因子」と「腫瘍免疫側の因子」との関連については不明な点が多い。
本検討では肝細胞癌のnon-coding RNAが腫瘍免疫に影響を及ぼす可能性について示した。今後、さらに広く行われる免疫療法の効果を増強することができる可能性が示唆された。
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