• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Single cell transcriptome analysis on circulating mesenchymal stem cells during tissue regeneration

Research Project

Project/Area Number 18K16027
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 53050:Dermatology-related
Research InstitutionOsaka University

Principal Investigator

Shimbo Takashi  大阪大学, 医学系研究科, 寄附講座准教授 (70780609)

Project Period (FY) 2018-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsシングルセルRNA-seq / 表皮水疱症 / 間葉系幹細胞 / 再生医療 / 骨髄由来PDGFRα陽性間葉系幹細胞 / 単一細胞網羅的遺伝子発現解析 / 組織再生 / single cell / rna-seq
Outline of Final Research Achievements

The supply of cells to damaged tissue is essential for functional tissue regeneration. Using a mouse model of epidermolysis bullosa, we found that the supply of bone marrow-derived PDGFRα-positive mesenchymal stem cells is important for tissue regeneration. In this project, we comprehensively identified functional subpopulations in circulating bone marrow-derived PDGFRα-positive mesenchymal stem cells involved in tissue regeneration by single-cell RNA-seq analysis. We successfully identified functional subpopulations in the bone marrow-derived PDGFRα-positive mesenchymal stem cell pool. We also demonstrated that bone marrow-derived PDGFRα-positive mesenchymal stem cells can differentiate and exert diverse functions in the mobilized damaged tissues.

Academic Significance and Societal Importance of the Research Achievements

間葉系幹細胞の高い抗炎症作用や分化能は、再生医療の分野で注目を集め内外で積極的に研究されている。我々が着目する生体に元来備わっている組織再生メカニズムを促進する組織再生誘導機構では、体外での幹細胞培養が不要であるため癌化のリスクはなく表皮水疱症新規治療法として有望である。組織再生誘導を担う末梢循環骨髄由来PDGFRα陽性間葉系幹細胞を単一細胞レベルで網羅的に解析し、その組織修復メカニズムを明らかにしようと試みる本研究は先駆的かつ革新的である。また末梢循環間葉系幹細胞には未解明な点が多く、生物学、発生学、幹細胞学の観点からも学術的価値は非常に高いと考える。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (6 results)

All 2019

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Transcriptionally distinct mesenchymal stem/stromal cells circulate in fetus2019

    • Author(s)
      Shimbo, T., Endo, M., Iwai; S., Kitayama, T., Ouchi, Y., Yamamoto, R., Takaki, E., Yamazaki, S., Nishida, M., Wang, X., Kikuchi, Y., Tomimatsu, T., Kaneda, Y., Kimura, T. Tamai, K
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: pii: S0006-291X(19) Issue: 2 Pages: 30410-3

    • DOI

      10.1016/j.bbrc.2019.03.033

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Single cell RNA-seq and single cell ATAC-seq analyses during a treatment for dystrophic epidermolysis bullosa2019

    • Author(s)
      Takashi Shimbo, Sho Yamazaki, Tomomi Kitayama, Yuya Ouchi, Ryoma Yamamoto, Eiichi Takaki, Leena Bruckner- Tuderman, Jouni Uitto, Yasufumi Kaneda, Katsuto Tamai
    • Organizer
      Society for Investigative Dermatology 77th
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] TRANSCRIPTOME AND EPIGENOME ANALYSES FOR REGENERATED SKIN IN A RECESSIVE DYSTROPHIC EPIDERMOLYSIS BULLOSA MOUSE MODEL2019

    • Author(s)
      Takashi Shimbo, Sho Yamazaki, Tomomi Kitayama, Yuya Ouchi, Ryoma Yamamoto, Eiichi Takaki, Leena Bruckner- Tuderman, Jouni Uitto, Yasufumi Kaneda, Katsuto Tamai
    • Organizer
      International Society for Stem Cell Research
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 網羅的単一細胞遺伝子発現解析が明らかにしたマウス潰瘍性大腸炎増悪メカニズム2019

    • Author(s)
      新保敬史、Ho Yen-Ting、北山智美、大内雄矢、Edward Wijaya、金田安史、玉井克人
    • Organizer
      第40回日本炎症・再生医学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Single cell transcriptome and epigenome analyses in a murine model of dystrophic epidermolysis bullosa2019

    • Author(s)
      Takashi Shimbo, Sho Yamazaki, Tomomi Kitayama, Yuya Ouchi, Ryoma Yamamoto, Eiichi Takaki, Leena Bruckner- Tuderman, Jouni Uitto, Yasufumi Kaneda, Katsuto Tamai
    • Organizer
      The 49th European Society for Dermatology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Single cell RNA- and ATAC-seq analyses in a murine model of dystrophic epidermolysis bullosa2019

    • Author(s)
      Takashi Shimbo, Sho Yamazaki, Tomomi Kitayama, Yuya Ouchi, Ryoma Yamamoto, Eiichi Takaki,Leena Bruckner-Tuderman, Jouni Uitto, Yasufumi Kaneda, Katsuto Tamai
    • Organizer
      第44回日本研究皮膚科学会
    • Related Report
      2019 Annual Research Report

URL: 

Published: 2018-04-23   Modified: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi