Optimization of large-scale culture methods for adipose tissue-derived mesenchymal stromal cell lines

• Project/Area Number: 18K16102
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 54010:Hematology and medical oncology-related
• Research Institution: Keio University
• Principal Investigator: URUGA Yukako 慶應義塾大学, 医学部(信濃町), 特任助教 (70464831)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2021: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 間葉系幹細胞

Outline of Final Research Achievements

Adipose tissue-derived mesenchymal stem cells (ASCs) are useful raw materials for regenerative medicine products. We have established an adipose tissue-derived mesenchymal stromal cell line (ASCL) from ASC, and aim to produce regenerative medical products using platelet-like cells (ASCL-PLC) which differentiated from ASCL. In this study, we developed a large-scale culture method of ASCLs for the manufacture of regenerative medical products. We were able to obtain platelet-like cells (ASCL-PLCs) from ASCLs and to determine specification and test methods of ASCL-PLCs. We believe that the results obtained in this study will accelerate the development of regenerative medicine products.

Academic Significance and Societal Importance of the Research Achievements

本研究成果は脂肪組織由来の血小板様細胞をはじめとした脂肪組織由来間葉系幹細胞を用いた再生医療等製品の製造への適用が可能であり、再生医療等製品の開発加速につながるものと考えられる。