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Identification of Mycoplasma hominis virulence factors using yeast expression system

Research Project

Project/Area Number 18K16187
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 54030:Infectious disease medicine-related
Research InstitutionKanazawa University (2023)
Kanazawa Medical University (2018-2022)

Principal Investigator

Kawai Yasuhiro  金沢大学, 医学系, 協力研究員 (10388936)

Project Period (FY) 2018-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsMycoplasma hominis / 病原因子 / マイコプラズマ属 / 薬剤耐性
Outline of Final Research Achievements

Based on the whole genome information of Mycoplasma hominis (ATCC23114), genes of unknown function were selected, and a pair of primers for each of them was prepared. ATCC23114 strain was cultured in liquid medium, and PCR products were obtained from the genome.
The experimental environment for the yeast expression system was completed.
Clinical isolates strains were examined for differences from the standard strain (ATCC23114). The standard strain and clinical isolates strains were cultured and MICs of various antimicrobial agents were measured. Although some of the clinical isolates strains showed values different from those of the standard strain without the previously reported mutations, we were able to find antimicrobial agents that could be expected to be effective even in these strains. We were able to find strains with altered susceptibility to antimicrobial agents and strains with very strong growth ability.

Academic Significance and Societal Importance of the Research Achievements

臨床分離株を用いて、既に機能が明らかにされている一部の遺伝子配列について解析を行い、菌株間での相違について検討を行った。M. hominisは他のMycoplasmaと異なり、第一選択薬となるマクロライド系抗菌薬に耐性を示す。抗菌薬耐性に関与する遺伝子配列について解析を行い、菌株間での相違について検討を行うために、臨床分離株の各種抗菌薬のMIC測定をした。一 部で既報の変異なく標準株と異なる値を示す株を認め、その株でも効果が期待できる抗菌薬を見出すことができた。また、非常に増殖力の強い菌株も見出すことができ、抗菌薬耐性や増殖に関する新たな遺伝子変異の同定に繋がる結果が得られた。

Report

(7 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (12 results)

All 2021 2020 2019 2018 Other

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (5 results) (of which Invited: 1 results) Book (3 results) Remarks (1 results)

  • [Journal Article] The JAID/JSC guidelines to Clinical Management of Infectious Disease 2017 concerning male urethritis and related disorders2021

    • Author(s)
      Hamasuna R, Yasuda M, Takahashi S, Uehara S, Kawai Y, Miyairi I, Arakawa S, Kiyota H
    • Journal Title

      Journal of Infection and Chemotherapy

      Volume: 27 Issue: 4 Pages: 546-554

    • DOI

      10.1016/j.jiac.2019.12.001

    • Related Report
      2021 Research-status Report 2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Blockade of endoplasmic reticulum stress‐induced cell death by Ureaplasma parvum vacuolating factor2021

    • Author(s)
      Nishiumi Fumiko、Kawai Yasuhiro、Nakura Yukiko、Yoshimura Michinobu、Wu Heng Ning、Hamaguchi Mitsuhide、Kakizawa Shigeyuki、Suzuki Yo、Glass John I.、Yanagihara Itaru
    • Journal Title

      Cellular Microbiology

      Volume: 23 Issue: 12 Pages: 1-19

    • DOI

      10.1111/cmi.13392

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] JAID/JSC感染症治療ガイドライン2018 男性尿道炎とその関連疾患2018

    • Author(s)
      濱砂 良一, 安田 満, 高橋 聡, 上原 慎也, 河合 泰宏, 宮入 烈, 荒川 創一, 清田 浩, 一般社団法人日本感染症学会・公益社団法人日本化学療法学会JAID/JSC感染症治療ガイド・ガイドライン作成委員会性感染症ワーキンググループ
    • Journal Title

      日本化学療法学会雑誌

      Volume: 66 Pages: 323-340

    • NAID

      130007839928

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] 金沢医科大学病院における抗菌薬適正使用支援についての検討2019

    • Author(s)
      河合泰宏
    • Organizer
      第93回日本感染症学会総会・学術講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] 当院におけるカンジダ血症の後方視的解析と予後因子の検討2019

    • Author(s)
      河合泰宏
    • Organizer
      第93回日本感染症学会総会・学術講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] 北陸地方における皮膚と軟部組織感染症から分離されたStaphylococcus aureusの遺伝学的特性(Genetic characterization of Staphylococcus aureus isolates from skin and soft tissue infections in Hokuriku district)2019

    • Author(s)
      Kawai Yasuhiro
    • Organizer
      第93回日本感染症学会総会・学術講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] カルバペネム系抗菌薬の初期治療および継続投与の適正性に関する検討2019

    • Author(s)
      河合泰宏
    • Organizer
      第67回日本化学療法学会総会
    • Related Report
      2019 Research-status Report
  • [Presentation] 耐性菌による市中感染にどう対峙するか マクロライド耐性マイコプラズマによる肺炎2018

    • Author(s)
      河合 泰宏
    • Organizer
      第92回日本感染症学会学術講演会・第66回日本化学療法学会総会 合同学会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Book] 小児疾患診療のための病態生理1 クラミジア感染症2020

    • Author(s)
      河合泰宏
    • Total Pages
      1171
    • Publisher
      東京医学社
    • Related Report
      2020 Research-status Report
  • [Book] JAID/JSC感染症治療ガイド 2019 XIII 性感染症2019

    • Author(s)
      清田浩, 荒川創一, 濱砂良一, 石地尚興, 上原慎也, 河合泰宏, 川名敬, 高橋聡, 三鴨廣繁, 安田満, 宮入烈
    • Total Pages
      351
    • Publisher
      ライフサイエンス出版株式会社
    • Related Report
      2019 Research-status Report
  • [Book] 改訂第9版 内科学書 vol. 2 感染経路 host-parasite relation ship2019

    • Author(s)
      河合泰宏, 飯沼由嗣
    • Total Pages
      598
    • Publisher
      中山書店
    • ISBN
      9784521747491
    • Related Report
      2019 Research-status Report
  • [Remarks] ウレアプラズマによる宿主細胞死の回避―気配を消す流早産原因菌の戦略―

    • URL

      https://www.amed.go.jp/news/seika/kenkyu/20211020-02.html

    • Related Report
      2022 Research-status Report 2021 Research-status Report

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Published: 2018-04-23   Modified: 2025-01-30  

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