| Project/Area Number |
18K16281
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 55010:General surgery and pediatric surgery-related
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| Research Institution | The University of Tokushima |
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Principal Investigator |
SAITO Yu 徳島大学, 病院, 特任助教 (50548675)
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| Project Period (FY) |
2018-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | Nrf2 inducer / 間葉系幹細胞 / インスリン産生細胞 / EGCG / Nrf2 / IPC / 分化誘導 |
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| Outline of Final Research Achievements |
The aim of this study was to investigate the effectiveness of 3D culture system with Nrf2 inducer on differentiation of human ADSCs to functional IPCs. EGCG was used as Nrf2 inducer. EGCG was added into Step 1 (germ layer conversion), Step 2 (IPC maturation), and Step 1/2. In terms with dose of EGCG, we have previously reported the islet protective effect of EGCG with 100uM dose (Surgery Today 2019), and that dose was also chosen in this study.
As a result, EGCG addition (100uM) decreased cell viabilities and insulin secretary function of IPCs. 100uM was thought to be cytotoxic for IPC differentiation. A low dose EGCG (10uM) also did not stimulate IPCs differentiation from ADSCs in both germ layer conversion and maturation steps.In conclusion, Nrf2 inducer, EGCG, did not stimulate IPC differentiation from ADSC. Further investigations were necessary to examine the EGCG effect for IPC differentiation.
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| Academic Significance and Societal Importance of the Research Achievements |
MSCは中胚葉性組織（間葉）に由来する体性幹細胞であり、通常は間葉系に属する細胞へと分化するが、微小環境の変化により中胚葉性でない組織にまで分化可能である。これまでに、MSCにNrf2を過剰発現させることで、骨芽細胞への分化誘導を促進することが報告されている。Nrf2 inducerが、MSCからIPCへの分化誘導の促進することで、低侵襲かつ迅速に移植が可能となり得ると考えられる。
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