Development of oncolytic virotherapy for maintaining long period effects of the immune checkpoint inhibitor

• Project/Area Number: 18K16327
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55020:Digestive surgery-related
• Research Institution: Wakayama Medical University
• Principal Investigator: Tsuji Toshiaki 和歌山県立医科大学, 医学部, 博士研究員 (20549737)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 抗がんウイルス / 胃がん / 免疫チェックポイント阻害 / 抗がんウイルス療法 / 治療ウイルス

Outline of Final Research Achievements

Development of gastric cancer therapy using oncolytic virus armed with immuno checkpoint inhibition is hopeful. We chose soluble PD-1 (sPD-1) gene for immune checkpoint inhibitory gene. We have started cloning of sPD-1 gene from c DNA library and blood sample, but we did not have obtained sPD-1 gene from neither of them. We are now continuing colonig of sPD-1.

Academic Significance and Societal Importance of the Research Achievements

本研究ではヒトsoluble PD-1 (sPD-1)遺伝子を抗がんヘルペスウイルスに導入して免疫チェックポイント阻害作用を持つ抗がんヘルペスウイルス開発を目的とし、sPD-1発現による抗腫瘍効果の増強が確認できれば動物実験を含めた前臨床的研究への発展が期待できるがsPD-1遺伝子のクローニングが予想よりも困難な状態で当初計画していた抗がんウイルス療法への応用が難しい。今後も研究継続を行うことにより抗がんウイルス療法の発展が期待される。

Research Products

• [Journal Article] Oncolytic virotherapy with SOCS3 enhances viral replicative potency and oncolysis for gastric cancer (2021)
  • Author(s): Matsumura Shuichi、Nakamori Mikihito、Tsuji Toshiaki、Kato Tomoya、Nakamura Masaki、Ojima Toshiyasu、Fukuhara Hiroshi、Ino Yasushi、Todo Tomoki、Yamaue Hiroki
  • Journal Title: Oncotarget Volume: 12 Issue: 4 Pages: 344
  • DOI: 10.18632/oncotarget.27873
  • Peer Reviewed / Open Access
• [Journal Article] Triangulating stapling vs functional end-to-end stapling for cervical esophagogastric anastomosis after esophagectomy for thoracic esophageal cancer: study protocol for a randomized controlled trial (2019)
  • Author(s): Tsuji T, Ojima T, Nakamori M, Nakamura M, Katsuda M, Hayata K, Kitadani J, Maruoka S, Shimokawa T, Yamaue H.
  • Journal Title: Trials Volume: 20 Issue: 1 Pages: 83-83
  • DOI: 10.1186/s13063-019-3201-2
  • Peer Reviewed / Open Access