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Hybrid lung tissue transplantation using skeletal muscle stroma-derived pluripotent stem cells and scaffolds

Research Project

Project/Area Number 18K16430
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 55040:Respiratory surgery-related
Research InstitutionTokai University

Principal Investigator

OIWA Kana  東海大学, 医学部, 助教 (30548045)

Project Period (FY) 2018-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords再生医療 / 肺組織移植 / 組織再灌流 / 気管移植 / 多能性幹細胞
Outline of Final Research Achievements

The realization of lung tissue transplantation is expected as one of the lung regenerative medicines for chronic progressive lung disease. The changes in graft were examined in detail. Hemoglobin vesicle was intravenously administered in a mouse tracheal parallel transplantation model, and the blood flow under the tracheal epithelium was observed. 0.3 ml of hemoglobin vesicle solution (Hb concentration: 10 g/dL) was administered via the tail vein, and then sacrificed at 3, 6, 12, and 24 hours, the trachea was cut out, and HE staining was performed to observe the tracheal cross section. Fine particles considered to be hemoglobin vesicles became visible in the subepithelial blood vessels by 3 hours after the operation, and reperfusion was confirmed. It was confirmed by observation with an electron microscope that the fine particles were hemoglobin vesicles. After 6 hours, reperfusion of red blood cells could be observed.

Academic Significance and Societal Importance of the Research Achievements

人工多機能幹細胞(iPS細胞)、或いは胚性幹細胞(ES細胞)を分化誘導させることにより、将来、肺を含む様々な組織、臓器の再生が可能となることが期待されている。in vitroで幹細胞を分化誘導させた後は、組織や細胞を各臓器に移植して生着させる必要がある。本研究により、生着率の高い肺組織移植法を確立できることが期待される。移植片中の細胞の詳しい動態が明らかになり、肺組織移植に関する基礎データの蓄積に貢献するであろう。将来、臨床応用につながる可能性もあり、肺移植以外の従来の方法では治療が困難であった慢性進行性肺疾患の新しい治療法に発展することが期待される。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (2 results)

All 2019 2018

All Presentation (2 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] Reperfusion of subepithelial capillaries after tracheal transplantation and systemic administration of hemoglobin vesicles in a mouse model.2019

    • Author(s)
      Hiroto Onozawa, Mitsutomo Kohno, Kana Oiwa, Ryo Hashimoto, Masazumi Watanabe, Hirohisa Horinouchi, Hiromi Sakai, Koichi Kobayashi, Masayuki Iwazaki
    • Organizer
      The 17th International Symposium on Blood Substitutes & Oxygen Therapeutics
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マウス気管移植後の上皮下血管の再潅流とヘモグロビン小胞体投与について2018

    • Author(s)
      小野沢博登 、橋本 諒、大岩加奈 、中川知己、河野光智、増田良太、 渡辺真純、堀之内宏久、酒井宏水、小林紘一、岩崎正之
    • Organizer
      第35回日本肺および心肺移植研究会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2021-02-19  

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