| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
We analyzed the function of the mutant NaV1.7 channel in patients with congenital insensitivity of pain. NaV1.7 channel function analysis was performed using the patch clamp method. It was confirmed the average peak current density of mutation channels was significantly smaller than Wild-Type channels, and the current-voltage relationship of mutation channels showed a tendency to shift slightly to the right of WT current-voltage relationship.
We evaluated the behavioral experiment with NaV1.7 mutant mice. For testing mechanical responses, calibrated von Frey filaments were applied．The response to mechanical noxious stimuli was found to be diminished. We also isolated the dorsal root ganglion from this mutant mouse and attempted to evaluate its electrophysiological function, but it was not measured within the study period.
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