Identification of genes associated with glioblastoma dissemination using whole genome sequencing

• Project/Area Number: 18K16569
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56010:Neurosurgery-related
• Research Institution: Kitasato University
• Principal Investigator: Ichiyo Shibahara 北里大学, 医学部, 講師 (30791016)
• Project Period (FY): 2018-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2021: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000); Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 膠芽腫 / 全エクソームシーケンス / 播種 / エクソーム解析 / RNAシーケンス

Outline of Final Research Achievements

Glioblastoma is a primary brain tumor with a dismal prognosis. Dissemination is an untreatable condition in glioblastoma; however, no studies have been conducted on this topic due to the missing of appropriate tumor samples. In this study, we conducted whole exome sequencing using the tumor samples of the initially disseminated glioblastoma. We could elucidate the molecular background of this rare tumor condition and establish successive research proposals to further elucidate the dissemination in the multi-omics ways.

Academic Significance and Societal Importance of the Research Achievements

膠芽腫は脳原発悪性腫瘍であるため、検体採取が極めて侵襲的である。これまで限られた検体で膠芽腫の病態解明を行わなければならなかった。さらに、播種病態は、腫瘍細胞が中枢神経系に散らばる病態であり、播種状態の検体採取は不可能であった。本研究では、独自の視点から、播種病態の検体採取を可能とし、且つ、その病態解明を試みる初めての研究である。当初は全エクソームシーケンス解析のみで病態解明が可能と考えていたが、マルチオミックス解析が急速に浸透している昨今、この播種病態研究においても同手法は必須である。本研究における独自のサンプリングを駆使し、播種病態解明をできる研究を進めることができている。

Research Products

• [Journal Article] Ventricular opening and cerebrospinal fluid circulation accelerate the biodegradation process of carmustine wafers suggesting their immunomodulation potential in the human brain (2022)
  • Author(s): Shibahara Ichiyo、Shibahara Yukiko、Hagiwara Hiroyuki、Watanabe Takashi、Orihashi Yasushi、Handa Hajime、Inukai Madoka、Hide Takuichiro、Yasui Yoshie、Kumabe Toshihiro
  • Journal Title: Journal of Neuro-Oncology Volume: 159 Issue: 2 Pages: 425-435
  • DOI: 10.1007/s11060-022-04078-5
  • Peer Reviewed / Open Access
• [Journal Article] Role of the parietooccipital fissure and its implications in the pathophysiology of posterior medial temporal gliomas (2022)
  • Author(s): Shibahara Ichiyo、Saito Ryuta、Kanamori Masayuki、Sonoda Yukihiko、Sato Sumito、Hide Takuichiro、Tominaga Teiji、Kumabe Toshihiro
  • Journal Title: Journal of Neurosurgery Volume: 137 Issue: 2 Pages: 505-514
  • DOI: 10.3171/2021.7.jns21990
  • Peer Reviewed
• [Journal Article] Long-term follow-up after BCNU wafer implantation in patients with newly diagnosed glioblastoma (2021)
  • Author(s): Shibahara I, Miyasaka K, Sekiguchi A, Ishiyama H, Inukai M, Yasui Y, Watanabe T, Sato S, Hide T, Kumabe T.
  • Journal Title: Journal of Clinical Neuroscience. Volume: 86 Pages: 202-210
  • DOI: 10.1016/j.jocn.2021.01.037
  • Peer Reviewed
• [Journal Article] Postcentral gyrus resection of opercular gliomas is a risk factor for motor deficits caused by damaging the radiologically invisible arteries supplying the descending motor pathway (2021)
  • Author(s): Shibahara Ichiyo、Sato Sumito、Hide Takuichiro、Saito Ryuta、Kanamori Masayuki、Sonoda Yukihiko、Tominaga Teiji、Kumabe Toshihiro
  • Journal Title: Acta Neurochirurgica Volume: - Issue: 5 Pages: 1269-1278
  • DOI: 10.1007/s00701-021-04737-y
  • Peer Reviewed
• [Journal Article] Temporal Glioblastoma Mimicking Basal Ganglia Invasion: Distinguishing Removable and Unremovable Tumors (2019)
  • Author(s): Ichiyo Shibahara 1, Mitsuto Hanihara, Kazuhiro Miyasaka, Sumito Sato, Takuichiro Hide, Toshihiro Kumabe
  • Journal Title: World Neurosurgery Volume: 130 Pages: e213-e221
  • DOI: 10.1016/j.wneu.2019.06.035
  • Peer Reviewed