| Project/Area Number |
18K16577
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 56010:Neurosurgery-related
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| Research Institution | University of Tsukuba |
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Principal Investigator |
プエンテス サンドラ 筑波大学, システム情報系, 助教 (00725765)
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| Project Period (FY) |
2018-04-01 – 2024-03-31
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| Project Status |
Granted (Fiscal Year 2022)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | Rodent stroke model / ROS scavenging / Brain edema / Stroke intervention / Cell therapy / Mesenchimal stem cells / Microglial polarization / Stroke / Mesenchymal stem cells / Ischemic penumbra / Microglia polarization / Modified stem cells / Motor recovery |
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| Outline of Annual Research Achievements |
During the FY2022, a blinded data analysis corresponding to the histochemistry performed on samples from rodent stroke models (mice) treated with different cell therapies (six groups in total (mesenchymal stem cells: young and old, with and without micro-vesicles, MVCs, and micro-vesicles only), plus a negative control (PBS)) has been carried over. Cell counting for multiple staining has been done to understand the changes in vascular, microglial, astrocytic, and neuronal population distributions in different areas compromised by the ischemic lesion. Data has been analyzed globally (total cell counting) and focally (sectioning into areas) to understand better the distribution of different cell populations. The cell counting and physiological data have been taken into account for animal distribution and grouping. Data from animals whose status may alter the results has been taken out from the statistics, and drafting is in process for a journal paper. Regarding in vitro analysis, different approaches to complement the data extracted from in-vitro experiments have been considered. Since the data analysis has not been over yet, dissemination of the results has been halted up to FY 2022.
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| Current Status of Research Progress |
Current Status of Research Progress
4: Progress in research has been delayed.
Reason
During COVID the sampling process was stopped and afterward we are deciding whether we can publish with the current data, or attempt to increase the number of animals. With the reduction of restrictions, we hope to proceed within the FY2023 to collect all the data (in vitro, and in vivo if needed) and publish a paper on it.
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| Strategy for Future Research Activity |
For FY2023, data analysis will be over along to in vitro testing of ROS scavenging under multiple conditions. If needed, in vivo experiments will be added to validate the groups. In parallel, paper drafting is ongoing to finalize a manuscript as soon as possible after the data collection and analysis is finalized.
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