| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
In this study, we analyzed the intestinal immune system and intestinal flora using a mouse collagen-induced arthritis (CIA) model with the goal of preventing the onset and relapse of rheumatoid arthritis (RA). After preparing the CIA model, immune cells were collected before and after the onset of CIA, after remission, and after relapse, and the kinetics and function were analyzed. Furthermore, joint tissues and blood were collected, and immunohistological evaluation and cytokine quantification were performed. In addition, feces were collected at any time, and intestinal microflora was analyzed using a next-generation sequencer. Immune cells, cytokines, and intestinal flora also fluctuated significantly with the onset, remission, and relapse of arthritis. In particular, it was suggested that the dynamics of the intestinal immune system differ between the initial onset and relapse.
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