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Regeneration of the intervertebral disc in the spine by Low Adhesive Scaffold Collagen (LASCol)

Research Project

Project/Area Number 18K16659
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 56020:Orthopedics-related
Research InstitutionKobe University

Principal Investigator

YURUBE Takashi  神戸大学, 医学部附属病院, 特定助教 (10514648)

Project Period (FY) 2018-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords椎間板変性 / 椎間板ヘルニア / LASCol / 脊椎 / 整形外科
Outline of Final Research Achievements

This study aimed to clarify effectiveness of the Low Adhesive Scaffold Collagen (LASCol) for intervertebral disc regeneration. The LASCol is a new collagen type I-based scaffold through actinidain hydrolysis with a high 3D spheroid-forming capability, water-solubility, and biodegradability and low antigenicity. In human disc cells surgically obtained, time-dependent spheroid formation with increased phenotypic markers and matrix components was observed on LASCol. In a rat tail nucleotomy model, LASCol-injected discs presented radiographic and MRI damage control. Then, LASCol decelerated histological disc disruption, showing aggrecan-positive and collagen type II-positive endogenous cell migration and macrophage infiltration. Reduced nucleotomy-induced disc disruption through spontaneous spheroid formation by LASCol warrants further investigations of whether it may be an effective treatment without stem cells and/or growth factors for intervertebral disc disease.

Academic Significance and Societal Importance of the Research Achievements

腰痛や関連した神経痛は世界的な健康問題であり、脊椎椎間板変性はその主たる要因である。現状の治療には限界があり、新たな戦略が必要と考えるに至った。破壊が進行した組織の再建には足場となる生体材料が重要であるが既存の材料は椎間板再生能を有していない。そのため本研究では新規に開発された細胞低接着性コラーゲン(Low Adhesive Scaffold Collagen:LASCol)による椎間板治療の可能性について検討した。結果、LASCol自体の細胞・組織誘導能を認め、椎間板再生への有用性が示された。得られた知見から新たな治療剤の開発に繋がる可能性があり、学術的・社会的に有意義であると考えられた。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (8 results)

All 2020 2019 2018

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (6 results) (of which Int'l Joint Research: 2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Reduced nucleotomy-induced intervertebral disc disruption through T spontaneous spheroid formation by the Low Adhesive Scaffold Collagen (LASCol)2020

    • Author(s)
      Yoshiki Takeoka, Takashi Yurube, Koichi Morimoto, Saori Kunii, Yutaro Kanda, Ryu Tsujimoto, Yohei Kawakasa Fukase, Toshiyuki Takemori, Kaoru Omae, Yuji Kakiuchi, Shingo Miyazaki, Kenichiro Kakutani, Toru Takada, Kotaro Nishidaa, Masanori Fukushimac, Ryosuke Kurodami, Naom
    • Journal Title

      Biomaterials

      Volume: 235 Pages: 119781-119781

    • DOI

      10.1016/j.biomaterials.2020.119781

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Regenerative potential of the damaged intervertebral disc by the Low Adhesive Scaffold Collagen (LASCol)2019

    • Author(s)
      Takeoka Y, Yurube T, Kunii S, Morimoto K, Fukase N, Takemori T, Kakiuchi Y, Kanda Y, Tsujimoto R, Kakutani K, Takada T, Kuroda R, Nishida K
    • Organizer
      The 65th Annual Meeting, Orthopaedic Research Society
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 細胞低接着性コラーゲン(Low Adhesive Scaffold Collagen:LASCol)による脊椎椎間板再生の可能性2018

    • Author(s)
      武岡由樹、由留部崇、國井沙織、森本康一、深瀬直政、竹森俊幸、垣内裕司、伊藤雅明、神田裕太郎、角谷賢一朗、高田徹、黒田良祐、西田康太郎
    • Organizer
      第47回日本脊椎脊髄病学会
    • Related Report
      2018 Research-status Report
  • [Presentation] Regenerative potential of the intervertebral disc by using the Low Adhesive Scaffold Collagen (LASCol)2018

    • Author(s)
      Takeoka Y, Yurube T, Kunii S, Morimoto K, Fukase N, Takemori T, Kakiuchi Y, Ito M, Kanda Y, Kakutani K, Takada T, Kuroda R, Nishida K
    • Organizer
      The 45th Annual Meeting, International Society for the Study of the Lumbar Spine
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 細胞低接着性コラーゲン(Low Adhesive Scaffold Collagen:LASCol)による脊椎椎間板再生2018

    • Author(s)
      武岡由樹、由留部崇、國井沙織、森本康一、竹森俊幸、深瀬直政、垣内裕司、角谷賢一朗、黒田良祐
    • Organizer
      第55回日本リハビリテーション医学会学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] 細胞低接着性コラーゲン(Low Adhesive Scaffold Collagen:LASCol)を用いた脊椎椎間板再生の試み2018

    • Author(s)
      武岡由樹、由留部崇、森本康一、國井沙織、深瀬直政、竹森俊幸、垣内裕司、角谷賢一朗、高田徹、黒田良祐、西田康太郎
    • Organizer
      第33回日本整形外科学会基礎学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] 細胞低接着性コラーゲン(LASCol)を用いた脊椎椎間板再生の可能性2018

    • Author(s)
      武岡由樹、由留部崇、國井沙織、森本康一、深瀬直政、竹森俊幸、垣内裕司、辻本龍、角谷賢一朗、髙田徹、黒田良祐、西田康太郎
    • Organizer
      第8回DDS再生医療研究会・第10回多血小板血漿(PRP)療法研究会
    • Related Report
      2018 Research-status Report
  • [Patent(Industrial Property Rights)] 椎間板変性の治療剤および椎間板細胞培養剤2018

    • Inventor(s)
      由留部 崇、武岡 由樹、森本 康一、國井 沙織、尾前 薫
    • Industrial Property Rights Holder
      由留部 崇、武岡 由樹、森本 康一、國井 沙織、尾前 薫
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2018-015541
    • Filing Date
      2018
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2021-02-19  

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