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Functional analysis of circulating tumor cells and epithelial-mesenchymal transition and inhibition of lung metastasis in osteosarcoma

Research Project

Project/Area Number 18K16679
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 56020:Orthopedics-related
Research InstitutionUniversity of Fukui (2020)
Osaka International Cancer Institute (2018-2019)

Principal Investigator

Tanaka Takaaki  福井大学, 学術研究院医学系部門, 講師 (00796245)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords骨肉腫 / 血中循環腫瘍細胞 / 上皮間葉移行 / 肺転移 / lung metastasis / circulating tumor cell / EMT
Outline of Final Research Achievements

The mouse osteosarcoma high lung metastasis strain LM8 and its parental strain Dunn were used. (1) LM8 was implanted subcutaneously in mice, and live CTCs were collected by direct floating culture of blood. The RNA was extracted from LM8 in 2D culture and LM8-CTC in 3D culture, and the difference in RNA was analyzed. (2) LM8 and Dunn were cultured in 2D culture, and the cell supernatant was collected for exosome analysis. (1) No difference in RNA between LM8 and LM8-CTC was observed. This may be due to the fact that CTCs consist of several to dozens of clusters, and the subtle differences in expression were eliminated by floating culture. However, in (2), we found a large difference in metastasis-related factor A between LM8 and Dunn.

Academic Significance and Societal Importance of the Research Achievements

骨肉腫は年間発生が約200例と非常に稀であり、若年齢層に好発する骨悪性腫瘍である。手術、化学療法、放射線治療を含めた集学的治療により5年生存率は8割程度であるが、肺転移例では2割程度と予後不良である。このため、骨肉腫治療においては原発巣のコントロールだけでなく、転移抑制を目的とした治療法が渇望されている。近年、転移形成において血中循環腫瘍細胞(CTC)の存在が重要な役割を果たすとの報告が多くされており、その機能を解析することは骨肉腫患者の予後改善につながることから意義のある研究である。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (18 results)

All 2021 2020 2019 2018

All Journal Article (10 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 10 results,  Open Access: 5 results) Presentation (6 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Book (2 results)

  • [Journal Article] The value of the black fiber sign on T1-weighted images for predicting stability of desmoid fibromatosis managed conservatively2020

    • Author(s)
      Murahashi Yasutaka、Emori Makoto、Shimizu Junya、Anzai Ken、Tanaka Takaaki、Naka Norifumi、Tsuchie Hiroyuki、Nagasawa Hiroyuki、Miyakoshi Naohisa、Shimada Yoichi、Yamashita Toshihiko
    • Journal Title

      European Radiology

      Volume: 30 Issue: 10 Pages: 5768-5776

    • DOI

      10.1007/s00330-020-06953-z

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Survival analysis of elderly patients with osteosarcoma2019

    • Author(s)
      Imura Yoshinori、Takenaka Satoshi、Kakunaga Shigeki、Nakai Takaaki、Wakamatsu Toru、Outani Hidetatsu、Tanaka Takaaki、Tamiya Hironari、Oshima Kazuya、Hamada Kenichiro、Naka Norifumi、Araki Nobuhito、Kudawara Ikuo、Ueda Takafumi、Yoshikawa Hideki
    • Journal Title

      International Orthopaedics

      Volume: 43 Issue: 7 Pages: 1741-1747

    • DOI

      10.1007/s00264-019-04332-y

    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Favorable outcomes of localized synovial sarcoma patients with a high utilization rate of neoadjuvant and/or adjuvant chemotherapy2019

    • Author(s)
      Outani Hidetatsu、Kakunaga Shigeki、Hamada Kenichiro、Takenaka Satoshi、Imura Yoshinori、Nagata Shigenori、Tanaka Takaaki、Tamiya Hironari、Oshima Kazuya、Naka Norifumi、Kudawara Ikuo、Araki Nobuhito、Ueda Takafumi、Yoshikawa Hideki
    • Journal Title

      Molecular and Clinical Oncology

      Volume: 11 Pages: 151-156

    • DOI

      10.3892/mco.2019.1863

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Establishment of a novel human CIC-DUX4 sarcoma cell line, Kitra-SRS, with autocrine IGF-1R activation and metastatic potential to the lungs2019

    • Author(s)
      Nakai Sho、Yamada Shutaro、Outani Hidetatsu、Nakai Takaaki、Yasuda Naohiro、Mae Hirokazu、Imura Yoshinori、Wakamatsu Toru、Tamiya Hironari、Tanaka Takaaki、Hamada Kenichiro、Tani Akiyoshi、Myoui Akira、Araki Nobuhito、Ueda Takafumi、Yoshikawa Hideki、Takenaka Satoshi、Naka Norifumi
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 15812-15812

    • DOI

      10.1038/s41598-019-52143-3

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Eribulin Suppresses Clear Cell Sarcoma Growth by Inhibiting Cell Proliferation and Inducing Melanocytic Differentiation Both Directly and Via Vascular Remodeling2019

    • Author(s)
      Nakai Sho、Tamiya Hironari、Imura Yoshinori、Nakai Takaaki、Yasuda Naohiro、Wakamatsu Toru、Tanaka Takaaki、Outani Hidetatsu、Takenaka Satoshi、Hamada Kenichiro、Myoui Akira、Araki Nobuhito、Ueda Takafumi、Yoshikawa Hideki、Naka Norifumi
    • Journal Title

      Molecular Cancer Therapeutics

      Volume: 19 Issue: 3 Pages: 742-754

    • DOI

      10.1158/1535-7163.mct-19-0358

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Clinical features and prognostic factors in patients with esophageal cancer with bone metastasis2019

    • Author(s)
      Imura Yoshinori、Yamamoto Sachiko、Wakamatsu Toru、Tanaka Takaaki、Tamiya Hironari、Sugimura Keijiro、Miyata Hiroshi、Ishihara Ryu、Yano Masahiko、Naka Norifumi
    • Journal Title

      Oncology Letters

      Volume: 19 Pages: 717-724

    • DOI

      10.3892/ol.2019.11142

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Clinical outcomes and significant factors in the survival rate after decompression surgery for patients who were non-ambulatory due to spinal metastases2019

    • Author(s)
      Tateiwa Daisuke、Oshima Kazuya、Nakai Takaaki、Imura Yoshinori、Tanaka Takaaki、Outani Hidetatsu、Tamiya Hironari、Araki Nobuhito、Naka Norifumi
    • Journal Title

      Journal of Orthopaedic Science

      Volume: 24 Issue: 2 Pages: 347-352

    • DOI

      10.1016/j.jos.2018.10.003

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Endoprosthetic Reconstruction for a Displaced Atypical Femoral Fracture in a Cancer Patient with Poor Prognosis2018

    • Author(s)
      Tamiya Hironari、Hagizawa Hiroki、Nakai Takaaki、Imura Yoshinori、Tanaka Takaaki、Oshima Kazuya、Ito Toshikazu、Naka Norifumi、Kuratsu Shigeyuki
    • Journal Title

      Case Reports in Orthopedics

      Volume: 2018 Pages: 1-5

    • DOI

      10.1155/2018/7862516

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Clinical Features and Prognosis of Patients With the Bone Metastasis of Pancreatic Cancer2018

    • Author(s)
      Outani Hidetatsu、Akita Hirofumi、Nakai Takaaki、Takada Ryoji、Imura Yoshinori、Tanaka Takaaki、Tamiya Hironari、Oshima Kazuya、Takahashi Hidenori、Ohkawa Kazuyoshi、Katayama Kazuhiro、Araki Nobuhito、Naka Norifumi
    • Journal Title

      Pancreas

      Volume: 47 Issue: 7 Pages: e43-e46

    • DOI

      10.1097/mpa.0000000000001098

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Calcific myonecrosis mimicking soft tissue sarcoma: A case report2018

    • Author(s)
      Ukon Yuichiro、Tanaka Takaaki、Nagata Shigenori、Hagizawa Hiroki、Imura Yoshinori、Tamiya Hironari、Oshima Kazuya、Naka Norifumi、Aoki Yasuaki、Kuratsu Shigeyuki
    • Journal Title

      Oncology Letters

      Volume: 15 Pages: 7909-7913

    • DOI

      10.3892/ol.2018.8320

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 骨軟部腫瘍はとにかく手術が面白い!チームビルディングの重要性2020

    • Author(s)
      田中 太晶
    • Organizer
      第53回日本整形外科学会骨・軟部腫瘍学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 第53回日本整形外科学会骨・軟部腫瘍学術集会2020

    • Author(s)
      田中 太晶
    • Organizer
      第62回日本小児血液・がん学会 学術集会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 大腿骨骨幹部転移性骨腫瘍に対する人工骨幹の使用経験2019

    • Author(s)
      田中 太晶
    • Organizer
      第52回日本整形外科学会 骨・軟部腫瘍学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] 右上腕骨骨肉腫に対して,Attractive BD-Scoreを用いてMRI、ADC値を定量的に測定し、腫瘍の活動性を評価した1例2019

    • Author(s)
      田中 太晶
    • Organizer
      第52回日本整形外科学会 骨・軟部腫瘍学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] Clinical experience of intercalary endoprosthetic reconstruction for malignant mid-diaphyseal bone tumors of femur and tibia2019

    • Author(s)
      Takaaki Tanaka
    • Organizer
      20th ISOLS 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Intramedullary nailing for bilateral femoral metastases from lung cancer and postoperative rehabilitation2018

    • Author(s)
      Takaaki Tanaka
    • Organizer
      12th Asia Pacific Musculoskeletal Tumor Society meeting
    • Related Report
      2018 Research-status Report
  • [Book] 手術看護エキスパート 2021 1・2月号2021

    • Author(s)
      田中 太晶
    • Total Pages
      110
    • Publisher
      日総研出版
    • Related Report
      2020 Annual Research Report
  • [Book] 手術看護エキスパート 2021 3・4月号2021

    • Author(s)
      田中 太晶
    • Total Pages
      110
    • Publisher
      日総研出版
    • Related Report
      2020 Annual Research Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

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