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Elucidation of somatic testicular cell abnormalities in male infertility and their application to new gene therapy

Research Project

Project/Area Number 18K16706
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 56030:Urology-related
Research InstitutionNagoya City University

Principal Investigator

NOZAKI SATOSHI  名古屋市立大学, 医薬学総合研究院(医学), 研究員 (50813432)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2020: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsライディッヒ細胞 / ホルモン異常 / 精巣毒性 / 男性不妊症 / 間質浮腫 / 男性不妊動物モデル / Setoli細胞 / Leydig細胞 / 無精子症 / Sertoli細胞 / 遺伝子導入
Outline of Final Research Achievements

Advances in assisted reproductive technology in male infertility have made remarkable progress, and surgery to collect intratesticular sperm under a microscope has become the standard treatment. However, sperm are not found in more than half, and it is necessary to establish a new treatment method. Therefore, we focused on testicular somatic cells rather than germ cells. As a result, we succeeded in isolating Leydig cells and clarified the mechanism of sperm dysfunction in hormonally abnormal male infertility model animals.

Academic Significance and Societal Importance of the Research Achievements

本研究成果により、精巣の体細胞であるライディッヒ細胞を効率よく分離することに成功した。また、LH-RH製剤を投与したホルモン異常男性不妊症モデル動物において、精巣間質の拡大とIL-6分泌の増加を明らかにした。今後、精巣間質の主体であるライディッヒ細胞をターゲットとする治療を行うことにより、次世代に伝播しない遺伝子治療が可能になると考える。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (5 results)

All 2021 2020 2018

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (3 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Efficacy of fosfomycin in the prevention of postoperative infection following transurethral resection of bladder tumor during periods of limited cefazolin, cefotiam, and cefmetazole supply2021

    • Author(s)
      Etani Toshiki、Asaoka Minami、Kondo Shuhei、Wachino Chiharu、Hori Kaoru、Shimizu Nobuhiko、Noda Yusuke、Nagai Takashi、Nozaki Satoshi、Iida Keitaro、Naiki Taku、Ando Ryosuke、Kawai Noriyasu、Yanagita Takeshi、Nakamura Atsushi、Yasui Takahiro
    • Journal Title

      Journal of Infection and Chemotherapy

      Volume: 27 Issue: 4 Pages: 625-631

    • DOI

      10.1016/j.jiac.2020.11.025

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Selective lysine-specific demethylase 1 inhibitor, NCL1, could cause testicular toxicity via the regulation of apoptosis2020

    • Author(s)
      Nozaki S, Naiki T, Naiki-Ito A, Iwatsuki S, Takeda T, Etani T, Nagai T, Iida K, Kato H, Suzuki T, Takahashi S, Umemoto Y, Yasui T.
    • Journal Title

      Andrology

      Volume: 8 Issue: 6 Pages: 1895-1906

    • DOI

      10.1111/andr.12846

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Selective lysine specific demethylase 1 inhibitor, NCL1, could cause testicular toxicity via the regulation of apoptosis2020

    • Author(s)
      Nozaki Satoshi, Naiki Taku, Iwatsuki Shoichiro, Naiki-Ito Aya, Takeda Tomoki, Etani Toshiki, Kurokawa Satoshi, Takahashi Satoru, Umemoto Yukihiro, Yasui Takahiro
    • Organizer
      American Urological Association Annual Meeting 2020(web開催)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] LHシグナルは精巣の間質浮腫に関与する2020

    • Author(s)
      野崎 哲史、岩月 正一郎、武田 知樹、窪田 裕樹、窪田 泰江、佐々木 昌一、梅本 幸裕、安井 孝周
    • Organizer
      第65回日本生殖医学会学術講演会・総会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Isolation of testicular somatic cells using DSA lectin and Percoll2018

    • Author(s)
      Satoshi Nozaki, Shoichiro Iwatsuki, Tomoki Takeda, Hiroki Kubota, Hiroyuki Kamiya, Shoichi Sasaki, Yukihiro Umemoto, Takahiro Yasui
    • Organizer
      第106回 日本泌尿器科学会総会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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