| Project/Area Number |
18K16735
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 56030:Urology-related
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| Research Institution | Osaka University |
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Principal Investigator |
Lu Yonggang 大阪大学, 免疫学フロンティア研究センター, 特任助教(常勤) (00817033)
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
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| Keywords | Male reproduction / Sperm-egg fusion / Fertilization / CRISPR/Cas9 / Knockout / Mouse model / Male contraception / Spermatology / male reproduction / male contraception / sperm / sperm-egg fusion / sperm function / sperm-oocyte fusion / Sperm / Spermatogenesis / Testis / Genome editing / Male fertility / Reproductive biology / Male infertility / Gene knockout |
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| Outline of Final Research Achievements |
During the four-year funding period, the applicant has generated multiple knockout mouse lines lacking testis-specific genes and analyzed the resultant phenotype. This study demonstrates the feasibility and efficiency of using the CRISPR/Cas9-mediated knockout strategy to identify genes essential for male reproduction. Through the large-scale in vivo screening, we have discovered essential genes Lrrc23, C2cd6, Sof1, Tmem95, and Fimp, which play roles in regulating sperm motility and sperm-egg fusion. The relevant research outcomes have been published in international peer-reviewed journals.
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| Academic Significance and Societal Importance of the Research Achievements |
My work provides explanations for idiopathic male infertility. The identified molecules may serve as biomarkers for male infertility and potential targets for non-hormonal male contraception.
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