Application of the regulation of endoplasmic reticulum stress to assisted reproductive technology
| Project/Area Number |
18K16790
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 56040:Obstetrics and gynecology-related
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| Research Institution | Yamagata University |
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Principal Investigator |
Takehara Isao 山形大学, 医学部, 客員研究員 (80642496)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 小胞体ストレス / 加齢卵 / 胚盤胞 / 胚発育 / 妊娠率 / 胚培養 / 胚移植 / 死細胞率 / salubrinal / 卵の質低下 |
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| Outline of Final Research Achievements |
The age-associated decline in female fertility is largely attributable to a decrease in oocyte quality caused by ovarian aging. In the present study, endoplasmic reticulum stress was strongly associated with aging of post-ovulatory aged mouse oocytes. Salubrinal, an inhibitor of the ER stress-related PERK pathway, significantly improved embryo development of aged mouse oocytes through suppression of blastomeres’ apoptosis. Based on our findings, we propose targeting, ER stress regulation as a new therapeutic strategy to improve oocyte quality.
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| Academic Significance and Societal Importance of the Research Achievements |
高齢女性の不妊治療の治療成績を改善させる治療は全く確立していないのが現状である。今回我々は、小胞体ストレス応答という生体内においてタンパク質の品質を維持する機構が過剰にはたらいてしまうことが卵の加齢による質の低下に関与していることを初めて明らかとした。さらに小胞体ストレス制御薬を使用することで治療成績が改善することをマウスを用いて証明した。この成果により、小胞体ストレス制御が難治性の高齢不妊の治療法に寄与する可能性が示唆された。
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Report
(4 results)
Research Products
(3 results)
