Effect of in vitro fertilization on fetal sex determination
| Project/Area Number |
18K16803
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 56040:Obstetrics and gynecology-related
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| Research Institution | Osaka University (2019)
The University of Tokushima (2018) |
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Principal Investigator |
Naoki Okashita 大阪大学, 生命機能研究科, 助教 (10757933)
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| Project Period (FY) |
2018-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 体外受精 / 体外培養 / 性決定 / エピゲノム / DNAメチル化 / Sry / 性転換 / エピジェネティクス |
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| Outline of Final Research Achievements |
We found that IVF enhances sex reversal of XY Jmjd1aΔ/Δ mice. Furthermore, we used molecular analyses and rescue studies to uncover the mechanisms underlying the IVF effect. Our results indicate that in vitro culture induces DNA hypermethylation at Sry promoter, and that this is maintained until the sex-determining period irrespective of DNA methylation reprogramming during early embryogenesis, and thus abnormal Sry induction is responsible for the sex reversal.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は、エピゲノムの観点から性決定メカニズムを理解する独自のテーマであり、学術的に価値のある成果に繋がる非常に意義のある研究である。さらに、本研究課題は学術的価値だけでなく医学的な意義も大きい。本課題の遂行によって、体外受精がエピゲノム修飾の構築エラーを誘発し、胎児の性決定に影響を及ぼすことが明らかになれば、生殖補助医療によるエピゲノム修飾の異常を起因とした疾患の発症メカニズム解明やその治療を目的とした研究の道しるべになることが期待される。
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Report
(3 results)
Research Products
(1 results)
