Analysis of microglia during developmental period in auditory system in animals for autism

• Project/Area Number: 18K16837
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56050:Otorhinolaryngology-related
• Research Institution: Jichi Medical University (2020-2021); Kanazawa University (2018-2019)
• Principal Investigator: NODA MASAO 自治医科大学, 医学部, 助教 (50756187)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2021: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: 聴覚中枢 / 自閉症 / グリア / 聴覚異常 / ミクログリア / 聴覚過敏

Outline of Final Research Achievements

We found that microglial morphological changes occur in the cochlear nerve nucleus, the auditory center, during auditory development, and that microglial morphology in the CD38KO mouse model of autism is different from that in the wild type.In addition, the autism model mice show abnormal myelination and synapse distribution in the auditory center, suggesting that these abnormalities may affect auditory development.

Academic Significance and Societal Importance of the Research Achievements

聴覚形成期に、聴覚中枢である蝸牛神経核においてミクログリアの形態変化が起きており、さらに自閉症モデルCD38KOマウスでは、野生型とは異なるミクログリアの形態変化が起きていることを明らかにした。
さらに、自閉症モデルマウスでは、聴覚中枢において髄鞘形成、シナプスの分布が異常であり、それらが、聴覚形成に影響している可能性が示唆された。
これらにより、自閉症患者における聴覚異常の病態解明と治療法開発にむけたターゲットになりうる。

Research Products

• [Journal Article] Deletion of CD38 and supplementation of NAD + attenuate axon degeneration in a mouse facial nerve axotomy model (2020)
  • Author(s): Takaso Y, Noda M, Hattori T, Roboon J, Hatano M, Sugimoto H, Brenner C, Yamamoto Y, Okamoto H, Higashida H, Ito M, Yoshizaki T, Hori O.
  • Journal Title: Sci Rep Volume: 10 Issue: 1 Pages: 17795-17795
  • DOI: 10.1038/s41598-020-73984-3
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Microglial activation in the cochlear nucleus after early hearing loss in rats. (2019)
  • Author(s): Noda M1, Hatano M2, Hattori T3, Takarada-Iemata M3, Shinozaki T4, Sugimoto H2, Ito M5, Yoshizaki T2, Hori O3.
  • Journal Title: Auris Nasus Larynx Volume: 46 Pages: 716-723
  • Peer Reviewed
• [Journal Article] Microglial activation in the cochlear nucleus after early hearing loss in rats. (2019)
  • Author(s): Noda M, Hatano M, Hattori T, Takarada-Iemata M, Shinozaki T, Sugimoto H, Ito M, Yoshizaki T, Hori O.
  • Journal Title: Auris Nasus Larynx. Volume: 印刷中 Issue: 5 Pages: 716-723
  • DOI: 10.1016/j.anl.2019.02.006
  • Peer Reviewed / Open Access
• [Presentation] Microglial activation in cpngenital deafness model rats (2019)
  • Author(s): Msao Noda
  • Organizer: 32th Politzer Society Meeting
  • Int'l Joint Research
• [Presentation] 聴覚発達期ラットの蝸牛神経核におけるミクログリアの変化と神経活動との関連についての検討 (2018)
  • Author(s): 野田昌生、波多野都、杉本寿史、伊藤真人、吉崎智一
  • Organizer: 第119回日本耳鼻咽喉科学会
• [Presentation] ラット顔面神経における末梢部位切断による顔面神経核ミクログリアの変化 (2018)
  • Author(s): 野田昌生、高相裕司、波多野都、杉本寿史、伊藤真人、吉崎智一
  • Organizer: 第41回日本顔面神経学会
• [Presentation] 難聴モデルラット蝸牛神経核におけるミクログリアの解析 (2018)
  • Author(s): 野田昌生、高相裕司、吉崎智一
  • Organizer: 第37回日本耳鼻咽喉科免疫アレルギー学会
• [Presentation] Microglial activation in the cochlear nucleus after early hearing loss in rats (2018)
  • Author(s): Noda M, Hatano M, Hattori T, Takarada M, Shinozaki T, Sugimoto H, Ito M, Yoshizaki T, Hori O
  • Organizer: Russia-Japan medical Symposium 2018
  • Int'l Joint Research
• [Presentation] Analysis of Microglia in Cochlear Nucleus in early hearing model rats (2018)
  • Author(s): Noda M, Hatano M, Hattori T, Takarada M, Shinozaki T, Sugimoto H, Ito M, Yoshizaki T, Hori O
  • Organizer: 34th World Congress of Audiology 2018
  • Int'l Joint Research