Analysis of human papillomavirus receptors in the head and neck region

• Project/Area Number: 18K16849
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56050:Otorhinolaryngology-related
• Research Institution: University of the Ryukyus
• Principal Investigator: Kinjo Hidetoshi 琉球大学, 病院, 助教 (00636417)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2021: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: ヒト乳頭腫ウイルス / 組織特異的感染メカニズム / 中咽頭癌 / 喉頭乳頭腫 / ヒトパピローマウイルス / 受容体 / 組織特異性

Outline of Final Research Achievements

This study was to elucidate the tissue-specific mechanism of human papillomavirus (HPV) by identifying the receptors. It was possible to produce high-risk HPV (HPV16 type) and low-risk HPV (HPV6 type, HPV11 type) virus like particle (VLP), which are frequently infected in the head and neck region. We tried to identify the receptor using virus over-ray protein binding assay (VOPBA) and immunoprecipitation method using the same VLP, but unfortunately we could not identify it, leaving a future task.

Academic Significance and Societal Importance of the Research Achievements

本研究ではHPVのVLP（ハイリスク型：HPV16型、ローリスク型：HPV6型、HPV11型）を作製することができた。しかし、残念ながらHPV（16型、6型、11型）の受容体を同定し、組織特異的感染メカニズムを解明することはできなかった。HPVの組織特異的感染メカニズムは免疫学的な監視からの逃避機構に関与している可能性もあると思われたため、今後の実験の課題をみつけることができた。