Comprehensive analysis of N-glycan in the the mammalian cochlea
Project/Area Number |
18K16877
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 56050:Otorhinolaryngology-related
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Research Institution | Niigata University |
Principal Investigator |
YORIKO NONOMURA 新潟大学, 医歯学総合研究科, 客員研究員 (60807022)
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Project Period (FY) |
2018-04-01 – 2022-03-31
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Project Status |
Completed (Fiscal Year 2021)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | N-結合型糖鎖 / 血管条 / 内耳蝸牛 / N-glycan / 蝸牛 / 内リンパ液 / N-結合型糖鎖 |
Outline of Final Research Achievements |
In this study, we comprehensively analyzed the profile of the N-linked glycans in the stria vascularis. Seventy-nine N-linked glycans were identified in the rat stria vascularis. Among these, in 55 glycans, the complete structures were determined; in the other 24 species, partial glycosidic linkage patterns and full profiles of the monosaccharide composition were identified. The high-mannose type was the most abundant and accounted for 38.1% of the total amount of strial glycans. Complex and hybrid types represented 34.8% and 21.0%, respectively. The least abundant variety was the paucimannose type, which represented 5.8% of the total. 43.6% of the total amount of N-glycans had single or multiple sialic acid residues; complex and hybrid types constituted 25.4% and 18.2%, respectively. Finally, core fucosylation was detected in 28.4% of the total amount of N-glycans, i.e. 2.6%, 23.3%, and 2.4% were the paucimannose type, complex type, and hybrid type of glycans, respectively.
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Academic Significance and Societal Importance of the Research Achievements |
聴覚機能のおける糖鎖の役割として、過去に報告されている主なものは、①糖脂質であるGM3の合成酵素欠損マウスでは、蝸牛の内、外有毛細胞が脱落し、難聴を呈する(Yoshikawa et al., PNAS 2009)、②ムンプス難聴においては、ムンプスウィルスがヒトに感染する際には、N-結合型糖鎖の構造の一部であるα2-3結合型シアル酸を含む構造が必要である(Kubota et al., PNAS 2016) などがある。これまで内耳に発現するN-結合型糖鎖を網羅的に解析した報告はない。本研究の成果は、発症のメカニズムが不明な内耳障害性難聴の病因解明や、治療開発に役立つと考えられる。
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Report
(5 results)
Research Products
(7 results)
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[Presentation] N-glycan profile of the epithelial-like tissue in the mammalian cochlea.2018
Author(s)
Yoriko Nonomura, Seishiro Sawamura, Fumiaki Nin, Satoru Uetsuka, Hidenori Inohara, Shujiro Okuda, Arata Horii, Sugata Takahashi, Shunji Natsuka, Hiroshi Hibino,
Organizer
31st Politzer Society Meeting & 2nd Global Otology Research Forum.
Related Report
Int'l Joint Research
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