ctHPVDNA monitoring for HPV-related head and neck cancer

• Project/Area Number: 18K16888
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56050:Otorhinolaryngology-related
• Research Institution: Osaka University
• Principal Investigator: Tanaka Hidenori 大阪大学, 医学系研究科, 特任講師(常勤) (00804379)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: Liquid biopsy / Oropharyngeal cancer / cfDNA / Human papillomavirus / Head and Neck cancer / HPV / Head and neck cancer / 中咽頭癌 / human papillomavirus / cell free DNA / digital PCR / liquid biopsy / oropharyngeal cancer / head and neck cancer / 個別化治療

Outline of Final Research Achievements

PET-CT and ctHPVDNA were compared in 35 cases in the response evaluation after radiotherapy. Although the negative predictive values were comparable, ctHPVDNA was found to have a superior positive predictive value.
Analysis of ctHPVDNA kinetics during radiotherapy in 22 cases revealed that the clearance pattern of ctHPVDNA could be classified into rapid and slow types. Furthermore, all cases with residual diseases were of the slow type.

Academic Significance and Societal Importance of the Research Achievements

ctHPVDNA検査はPET-CTと同等の診断精度を有する可能性が示唆された。本検査は少量の採血から検査できることで低侵襲な検査法として期待される。また、放射線治療中のctHPVDNAのクリアランスパターンを解析することで、より適切な線量の放射線治療を行える可能性がある。これはctHPVDNAが精密医療や低侵襲治療における重要なバイオマーカーとなる可能性を示唆している。

Research Products

• [Journal Article] Performance of oral HPV DNA, oral HPV mRNA and circulating tumor HPV DNA in the detection of HPV‐related oropharyngeal cancer and cancer of unknown primary (2021)
  • Author(s): Tanaka Hidenori、Suzuki Motoyuki、Takemoto Norihiko、Fukusumi Takahito、Eguchi Hirotaka、Takai Erina、Kanai Haruka、Tatsumi Mitsuaki、Horie Masafumi、Takenaka Yukinori、Yachida Shinichi、Inohara Hidenori
  • Journal Title: International Journal of Cancer Volume: 150 Issue: 1 Pages: 174-186
  • DOI: 10.1002/ijc.33798
  • Peer Reviewed / Open Access
• [Journal Article] Circulating tumor HPV DNA complements PET‐CT in guiding management after radiotherapy in HPV ‐related squamous cell carcinoma of the head and neck (2020)
  • Author(s): Tanaka Hidenori、Takemoto Norihiko、Horie Masafumi、Takai Erina、Fukusumi Takahito、Suzuki Motoyuki、Eguchi Hirotaka、Komukai Sho、Tatsumi Mitsuaki、Isohashi Fumiaki、Ogawa Kazuhiko、Yachida Shinichi、Inohara Hidenori
  • Journal Title: International Journal of Cancer Volume: 148 Issue: 4 Pages: 995-1005
  • DOI: 10.1002/ijc.33287
  • Peer Reviewed
• [Journal Article] SuccessfulcontrolofT4andN3humanpapillomavirus-relatedoropharyngealsquamouscell carcinomaafterde-intensifiedchemoradiotherapy:Reportoftwocases (2019)
  • Author(s): Hidenori Tanaka, Yoichiro Tomiyama, Takahiro Michiba, Takahito Fukusumi, Norihiko Takemoto, Motoyuki Suzuki, Hidenori Inohara
  • Journal Title: Oral Oncology Volume: 97 Pages: 146-148
  • Peer Reviewed
• [Presentation] Diagnostic Performance of Oral HPV DNA, Oral HPV mRNA, and Circulating Tumor HPV DNA for HPV-Related Oropharyngeal Cancer and Cancer of Unknown Primary (2021)
  • Author(s): Hidenori Tanaka, Motoyuki Suzuki, Norihiko Takemoto, Takahito Fukusumi, Hirotaka Eguchi, Erina Takai, Haruka Kanai, Mitsuaki Tatsumi, Masafumi Horie, Shinichi Yachida, Hidenori Inohara
  • Organizer: IPVC2021
  • Int'l Joint Research
• [Presentation] HPV感染からみる中咽頭癌の現在の問題点と予防戦略 (2020)
  • Author(s): 田中秀憲 曺 弘規 江口 博孝 福角 隆仁 武本 憲彦 鈴木 基之 猪原 秀典
  • Organizer: 第58回日本癌治療学会
  • Invited
• [Presentation] HPV関連中咽頭癌の個別化治療に向けたHPV cfDNAモニタリングの意義 (2019)
  • Author(s): 田中秀憲 佐々木崇博 福角隆仁 武本憲彦 鈴木基之 猪原秀典
  • Organizer: 第43回 日本頭頸部癌学会
  • Invited
• [Presentation] Plasma HPV cell-free DNA and HPV-related HNSCC (2018)
  • Author(s): Hidenori Tanaka,T Sasaki, T Fukusumi, N Takemoto, M Suzuki, H Inohara
  • Organizer: EUROGIN
  • Int'l Joint Research
• [Presentation] 遠隔転移を有するHPV関連頭頸部癌症例の検討 (2018)
  • Author(s): 田中秀憲 山本雅司 道場隆博 武本憲彦 鈴木基之 猪原秀典
  • Organizer: 日本頭頸部癌学会
• [Funded Workshop] IPVC2021 (2021)
• [Funded Workshop] EUROGIN (2018)