Mechanism of laryngeal papilloma formation by HPV: an approach from growth control and immune evasion system
| Project/Area Number |
18K16896
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 56050:Otorhinolaryngology-related
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
Ikegami Taro 琉球大学, 医学(系)研究科(研究院), 助教 (00754409)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 喉頭乳頭腫 / HPV-6 / ヒトパピローマウイルス / HPV / in situ hybridization / 抗体作製 / laryngeal papillomatosis / 声帯 / HPV-11 / 成長因子 / 免疫 |
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| Outline of Final Research Achievements |
HPV-6 has nine genes. In HPV-6-infected laryngeal papilloma, E4, E5a and E5b accounted for 96% of the total viral gene expression. Interestingly, these three genes were synchronizedly expressed in the same cells of laryngeal papillomas. Since the E4 gene was the most expressed, we produced an anti-HPV-6E4 antibody and proved that the E4 protein is the major expressed in papillomas. These results suggest that E4, E5a and E5b play an important role in the formation and maintenance of laryngeal papillomas.
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| Academic Significance and Societal Importance of the Research Achievements |
これまで喉頭乳頭腫においてHPV全遺伝子の発現および発現部位など詳細に調べられていなかった。本研究は、全遺伝子の発現プロファイルと発現部位を明らかにしたことより、喉頭乳頭腫におけるウイルスの動態の全体像を明らかにした。特に、E4、E5aおよびE5bが乳頭腫形成に重要な働きをしていることが示唆された。HPV-6 E4についてはこれまで抗体がなかったが、今回我々が世界で初めてモノクローナル抗体の作製にも成功した。今後、これらの成果をもとに治療法などの開発をして、臨床に還元していきたい。
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Prognostic significance of hypoxia-inducible factor-1α expression in advanced pharyngeal cancer without human papillomavirus infection2021
Author(s)
Agena S , Hirakawa H , Ikegami T , Kinjyo H , Kise N , Maeda M , Uezato J , Kondo S , Kiyuna A , Yamashita Y , Hasegawa N , Suzuki M , Ganaha A
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Journal Title
Journal of Laryngology and Otology
Volume: 印刷中
Related Report
Peer Reviewed / Open Access
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[Journal Article] 下咽頭癌におけるDNA修復酵素遺伝子多型と発癌についての検討2020
Author(s)
疇地里衣, 平川仁, 池上太郎, 長谷川成海, 山下懐, 安慶名信也, 上原貴行, 田中克典, 上里迅, 真栄田裕行, 鈴木幹男
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Journal Title
耳鼻と臨床
Volume: 66
Pages: 35-40
NAID
Related Report
Peer Reviewed
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[Journal Article] ERCC1 C8092A polymorphism predicts fair survival outcome in Japanese patients with pharyngo-laryngeal squamous cell carcinoma2020
Author(s)
Hirakawa H, Ikegami T, Azechi S, Agena S, Uezato J, Kinjyo H, Yamashita Y, Tanaka K, Kondo S, Maeda H, Suzuki M, Gahana A
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Journal Title
European Archives of Oto-Rhino-Laryngology
Volume: 277
Issue: 2
Pages: 601-610
DOI
Related Report
Peer Reviewed
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