Development of cell transplantation therapy using pluripotent stem cells for severe vestibular disorders
Project/Area Number |
18K16903
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 56050:Otorhinolaryngology-related
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Research Institution | Nara Medical University |
Principal Investigator |
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Project Period (FY) |
2018-04-01 – 2020-03-31
|
Project Status |
Completed (Fiscal Year 2019)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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Keywords | 内耳有毛細胞 / ES細胞 / 内耳再生 / 分化誘導 / 前庭 / 高度前庭障害 / マウス |
Outline of Final Research Achievements |
Vestibular hair cells (V-HCs) in the inner ear have important roles and various functions. When V-HCs are damaged, crippling symptoms, such as vertigo, visual field oscillation, and imbalance, are often seen. Recently, several studies have reported differentiation of embryonic stem (ES) cells, as pluripotent stem cells, to HCs, though a method for producing V-HCs has yet to be established. In the present study, we used vestibular cell conditioned medium (V-CM) and effectively induced ES cells to differentiate into V-HCs. Expressions of V-HCrelated markers (Math1, Myosin6, Brn3c, Dnah5) were significantly increased in ES cells cultured in V-CM for 2weeks, while those were not observed in ES cells cultured without V-CM. On the other hand, the cochlear HCrelated marker Lmod3 was either not detected or detected only faintly in those cells when cultured in V-CM. This results demonstrate that V-CM has an ability to specifically induce differentiation of ES cells into V-HCs.
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Academic Significance and Societal Importance of the Research Achievements |
本研究により、前庭細胞由来液性因子を用いることで、前庭有毛細胞への特異的分化誘導制御が実現可能となった。この新しい知見は、内耳再生医学の基礎的視点から前庭有毛細胞の分化・再生メカニズムを解明する上で重要と考えられる。
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Report
(3 results)
Research Products
(4 results)