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Elucidation of the role of the cytoskeleton in mitochondrial dysfunction in cochlear cells

Research Project

Project/Area Number 18K16906
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 56050:Otorhinolaryngology-related
Research InstitutionThe University of Tokyo

Principal Investigator

Kamogashira Teru  東京大学, 医学部附属病院, 助教 (30807152)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsミトコンドリア / 動的評価 / 細胞骨格 / PQQ / 老化 / 拡散係数 / 蝸牛 / 有毛細胞
Outline of Final Research Achievements

The mitochondrial movement in the cochlea cell line (HEI-OC1) was continuously tracked by extracting feature points and calculating optical flow using OpenCV, calculating diffusion coefficients, etc. The same coefficients were calculated after exposure to cytoskeletal inhibitors, and dynamic evaluation was performed. The mitochondrial membrane potential was evaluated by plate reader and fluorescence microscopy. The morphology of mitochondria was evaluated by electron microscopy. After exposure to PQQ (0.1, 1.0 nM) for 1 day, the cells were exposed to hydrogen peroxide (100 μM) for 1 hour to evaluate mitochondrial function and mitochondrial area. SIRT1/PGC-1α pathway functions were evaluated.

Academic Significance and Societal Importance of the Research Achievements

蝸牛培養細胞株(HEI-OC1)老化モデルでのミトコンドリア呼吸能、ATP産生速度、ミトコンドリア膜電位・融合・運動性の障害はPQQ処理群で保護され、SIRT1及びPGC-1αの発現は有意に上昇し、PGC-1αのアセチル化は有意に減少した。ミトコンドリア拡散係数が増加する傾向にあり、動的な側面が改善する可能性が示唆された。
細胞老化においてPQQにより細胞骨格の観点からの評価を含めたミトコンドリア機能が改善することが示唆され、加齢性難聴に対して有効な薬剤であることが推測され、同内容にて特許出願を行った。本内容は、高齢化社会における難聴予防の点からも学術的意義や社会的意義があると考えられる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (24 results)

All 2021 2020 2019 2018

All Journal Article (11 results) (of which Peer Reviewed: 10 results,  Open Access: 8 results) Presentation (11 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Alteration of vestibular function in pediatric cochlear implant recipients2021

    • Author(s)
      Hajime Koyama, Akinori Kashio, Chisato Fujimoto, Tsukasa Uranaka, Yu Matsumoto, Teru Kamogashira, Makoto Kinoshita, Shinichi Iwasaki and Tatsuya Yamasoba
    • Journal Title

      Frontiers in Neurology

      Volume: -

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 嚥下CTを用いた嚥下機能検査のバーチャルリアリティー技術利用の試み2021

    • Author(s)
      上羽瑠美、鴨頭輝、後藤多嘉緒、佐藤拓、山内彰人、山岨達也
    • Journal Title

      嚥下医学

      Volume: -

    • NAID

      40022701838

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Utriculo-ocular pathway dysfunction is more frequent in vestibular migraine than probable vestibular migraine.2020

    • Author(s)
      Fujimoto C, Kamogashira T, Takenouchi S, Kinoshita M, Sugasawa K, Kawahara T, Yamasoba T, Iwasaki S
    • Journal Title

      J Neurol

      Volume: 267 Issue: 8 Pages: 2340-2346

    • DOI

      10.1007/s00415-020-09851-y

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Zone-specific damage of the olfactory epithelium under protein restriction2020

    • Author(s)
      Tuerdi Ayinuer、Kikuta Shu、Kinoshita Makoto、Kamogashira Teru、Kondo Kenji、Yamasoba Tatsuya
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 22175-22175

    • DOI

      10.1038/s41598-020-79249-3

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Prediction of Vestibular Dysfunction by Applying Machine Learning Algorithms to Postural Instability2020

    • Author(s)
      Kamogashira Teru、Fujimoto Chisato、Kinoshita Makoto、Kikkawa Yayoi、Yamasoba Tatsuya、Iwasaki Shinichi
    • Journal Title

      Frontiers in Neurology

      Volume: 11 Pages: 1-1

    • DOI

      10.3389/fneur.2020.00007

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Rhinolithiasis caused by Actinomyces with a foreign body2020

    • Author(s)
      Kamogashira Teru、Matsumoto Naoyuki、Numakura Satoe、Kikuchi Yoshinao、Ito Ken
    • Journal Title

      IDCases

      Volume: 19 Pages: e00718-e00718

    • DOI

      10.1016/j.idcr.2020.e00718

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of a Web software for drawing nystagmograms2019

    • Author(s)
      Ishida Yosuke、Ito Ken、Kamogashira Teru、Murofushi Toshihisa
    • Journal Title

      Equilibrium Research

      Volume: 78 Issue: 6 Pages: 602-606

    • DOI

      10.3757/jser.78.602

    • NAID

      130007795215

    • ISSN
      0385-5716, 1882-577X
    • Year and Date
      2019-12-31
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of the headphone characteristics on the ABR and the VEMP2019

    • Author(s)
      Asakura Shinnosuke、Kamogashira Teru、Ishimoto Shinichi、Yamasoba Tatsuya
    • Journal Title

      AUDIOLOGY JAPAN

      Volume: 62 Issue: 6 Pages: 632-638

    • DOI

      10.4295/audiology.62.632

    • NAID

      130007795196

    • ISSN
      0303-8106, 1883-7301
    • Year and Date
      2019-12-28
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 音声コーパスをもとにした長文節の聞き取り検査及び自動判定システムの開発2019

    • Author(s)
      鴨頭輝, 樫尾明憲, 西村信一, 山岨達也
    • Journal Title

      AUDIOLOGY JAPAN

      Volume: 62 Issue: 5 Pages: 554

    • DOI

      10.4295/audiology.62.554

    • NAID

      130007751946

    • ISSN
      0303-8106, 1883-7301
    • Year and Date
      2019-10-30
    • Related Report
      2019 Research-status Report
  • [Journal Article] Noisy galvanic vestibular stimulation has a greater ameliorating effect on posture in unstable subjects: a feasibility study2019

    • Author(s)
      Fujimoto Chisato、Kinoshita Makoto、Kamogashira Teru、Egami Naoya、Kawahara Takuya、Uemura Yukari、Yamamoto Yoshiharu、Yamasoba Tatsuya、Iwasaki Shinichi
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 7-7

    • DOI

      10.1038/s41598-019-53834-7

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dorsal-zone-specific reduction of sensory neuron density in the olfactory epithelium following long-term exercise or caloric restriction2018

    • Author(s)
      Tuerdi Ayinuer、Kikuta Shu、Kinoshita Makoto、Kamogashira Teru、Kondo Kenji、Iwasaki Shinichi、Yamasoba Tatsuya
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 17300-17300

    • DOI

      10.1038/s41598-018-35607-w

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] ピロロキノリンキノン(PQQ)の蝸牛細胞株の老化モデルにおけるミトコンドリア機能の保護効果2021

    • Author(s)
      鴨頭輝、藤本千里、岩崎真一、山岨達也
    • Organizer
      第122回 日本耳鼻咽喉科学会総会・学術講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 加齢性難聴モデルにおけるピロロキノリンキノン(PQQ) の保護効果2021

    • Author(s)
      鴨頭輝、藤本千里、岩崎真一、山岨達也
    • Organizer
      第21回 日本抗加齢医学会総会
    • Related Report
      2020 Annual Research Report
  • [Presentation] VEMP検査に伴う音響外傷の症例2020

    • Author(s)
      朝倉信之介、鴨頭輝
    • Organizer
      第79回 日本めまい平衡医学会総会・学術講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 時間帯制限給餌下でのゲンタマイシン全身投与による前庭障害特異的モデルマウスの確立2020

    • Author(s)
      鴨頭輝、藤本千里、山岨達也、岩崎真一
    • Organizer
      第79回 日本めまい平衡医学会総会・学術講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 誘発電位検査装置用の各種耳挿入型イヤホンの音響特性とその問題点2020

    • Author(s)
      朝倉信之介、鴨頭輝、石本晋一、山岨達也
    • Organizer
      第65回 日本聴覚医学会総会・学術講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 蝸牛細胞株の老化モデルにおけるミトコンドリア運動性と細胞骨格の解析2020

    • Author(s)
      鴨頭輝、林賢、藤本千里、岩崎真一、山岨達也
    • Organizer
      第121回 日本耳鼻咽喉科学会総会・学術講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 当院におけるめまい症例の検討2019

    • Author(s)
      朝倉信之介,鴨頭 輝
    • Organizer
      第78回日本めまい平衡医学会総会学術講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] 音声コーパスをもとにした長文節の聞き取り検査及び自動判定システムの開発2019

    • Author(s)
      鴨頭輝, 樫尾明憲, 西村信一, 山岨達也
    • Organizer
      第64回日本聴覚医学会総会・学術講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] めまい専門外来の症例における機械学習での疾患予測の評価2018

    • Author(s)
      鴨頭輝、木下淳、藤本千里、江上直也、牛尾宗貴、菅澤恵子、岩崎真一、山岨達也
    • Organizer
      第77回日本めまい平衡医学会総会・学術講演会
    • Related Report
      2018 Research-status Report
  • [Presentation] ABRにおけるIEC60318-3及びIEC60318-1準拠ヘッドホンの比較2018

    • Author(s)
      朝倉信之介、鴨頭輝、石本晋一、山岨達也
    • Organizer
      第63回日本聴覚医学会総会・学術講演会
    • Related Report
      2018 Research-status Report
  • [Presentation] 鼻腔異物を伴う鼻腔放線菌症の一例2018

    • Author(s)
      鴨頭輝、安井拓也、平野真希子、伊藤健
    • Organizer
      第119回日本耳鼻咽喉科学会総会・学術講演会
    • Related Report
      2018 Research-status Report
  • [Patent(Industrial Property Rights)] 騒音性難聴の予防剤2020

    • Inventor(s)
      鴨頭輝、山岨達也、藤本千里、他3名
    • Industrial Property Rights Holder
      鴨頭輝、山岨達也、藤本千里、他3名
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2020-184254
    • Filing Date
      2020
    • Related Report
      2020 Annual Research Report
  • [Patent(Industrial Property Rights)] ポータブル両側鼻腔通気度計(明細書名:鼻腔通気度検査装置)2018

    • Inventor(s)
      鴨頭輝
    • Industrial Property Rights Holder
      鴨頭輝
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2018-033096
    • Filing Date
      2018
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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