| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
In this research, STR assay and repeat primed PCR were performed on the DNA of 41 outpatients with Fuchs corneal endothelial dystrophy, and repeat elongation of TCF4 was observed in 8 patients (19.5%).
We succeeded in cleaving the repeat of the immortalized corneal endothelial cell HCEnC using CRISPR-Cas9, and succeeded in creating a knock-out cell line. We also succeeded in producing knock-in cell lines for HCEnC with 60-repeat and 80-repeat donors using the Homology direct repair method. RNA foci was observed in the 80 repeat knock-in cell line, and TCF4 gene expression by RT-PCR was increased.
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