| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Retinal dystrophy is thought to be caused not only by a gene mutation but by a dysregulated gene expression through an epigenetic mechanism, including a histone modification. To clarify the contribution of H3K27me3, a repressive histone modification, to retinal dystrophy, MNU, which causes photoreceptor degeneration, was administered to a control and a conditional knockout of Ezh2 (Ezh2-cKO), a methyltransferase for H3K27me3, mouse and retinas were analyzed by immunohistochemistry and RT-qPCR. Compared with the control, the Ezh2-cKO retina showed milder photoreceptor degeneration, especially in male mice, and the expression of a gene related to necroptosis, a programmed form of necrosis, was lower in the Ezh2-cKO retina. The results suggest the contribution of H3K27me3 to necroptosis of photoreceptor cells during retinal degeneration.
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