| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2021: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Circular corneal epithelial defects were created in mice, and wound healing and cell proliferation were examined. There was no statistically significant difference in the residual area of the corneal epithelial defect between ShhΔCE and Shhf/wt mice, and the number of BrdU-positive cells was significantly suppressed in the corneal epithelium of ShhΔCE mice 6 hours after corneal epithelial detachment compared to that of Shhf/wt mice. Immunostaining for Sox9 expression during corneal epithelial wound healing revealed enhanced expression of Sox9 in the migrating corneal epithelium of ShhΔCE mice at 12 hours after corneal epithelial detachment compared to Shhf/wt mice. Whole-mount immunostaining of the cornea with TUJ1 antibody showed suppression of corneal nerve distribution and hypersensitivity.
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