The role of Semaphorin7a in the trophic effect of stem cells

• Project/Area Number: 18K17045
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57030:Conservative dentistry-related
• Research Institution: Osaka University
• Principal Investigator: Sawada Keigo 大阪大学, 歯学研究科, 特任研究員 (70733054)
• Project Period (FY): 2018-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 歯周病 / 歯周組織再生 / 間葉系幹細胞 / Trophic効果 / Semaphorin7a / 歯周組織 / 再生医学 / 歯学 / 幹細胞

Outline of Final Research Achievements

In this work, we analyzed the effects of Semaphorin7a, a Semaphorin family molecule secreted by adipose tissue-derived multisystemic progenitor cells (ADMPCs), on the cellular function of human periodontal pulp cells (HPDLs). First, we examined whether Semaphorin7a affected the cell proliferation and migration ability of HPDL, and found that recombinant Semaphorin7a promoted the cell proliferation ability of HPDL but did not affect cell migration ability. Next, we examined whether Semaphorin7a affected the differentiation potential of HPDL and found that recombinant Semaphorin7a up-regulated the expression of hard tissue formation-related genes in HPDL. These results indicate that Semaphorin7a activates the cellular function of periodontal cells.

Academic Significance and Societal Importance of the Research Achievements

本研究では、これまで明らかにされてこなかった間葉系幹細胞が分泌するSemaphorin7aによる歯周組織の細胞機能に与える役割を明らかにした。この結果から、ADMPC由来の Trophic因子による歯周組織再生誘導機序の一端が明らかになり、特に間葉系幹細胞由来のSemaphorin7aが組織再生へ与える効果が明らかとなった。これらの知見は、今後の間葉系幹細胞移植による組織再生機序の解明に寄与するものであり、本研究成果は重要な学術的意義および社会的意義を有すると考えられる。

Research Products

• [Presentation] 脂肪組織由来多系統前駆細胞(ADMPC)由来Trophic因子のプロテオーム解析 (2019)
  • Author(s): 沢田啓吾、竹立匡秀、森本千晶、平井麻絵、下村純平、川嵜公輔、岩山智明、北村正博、村上伸也
  • Organizer: 日本プロテオーム学会2019年大会
• [Presentation] Shotgun Proteomic Analysis of the humoral factors derived from ADMPC (2019)
  • Author(s): Sawada K, Takedachi M, Morimoto C, Hirai A, Shimomura J, Kawasaki K, Iwayama T, Kitamura M, Murakami S
  • Organizer: 4th Meeting of the International Association for Dental Research Asia Pacific Region 2019
  • Int'l Joint Research
• [Presentation] Clinical Evaluation of Periodontal Tissue Regeneration by ADMPC Transplantation (2018)
  • Author(s): Masahide Takedachi, Keigo Sawada, Chiaki Morimoto, Satomi Yamamoto, Asae Hirai, Junpei Shimomura, Yuki Narukawa, Yoichiro Kashiwagi, Tomoaki Iwayama, Masahiro Kitamura, Shinya Murakami
  • Organizer: 2018 IADR GENERAL SESSION
• [Presentation] Therapeutic efficacy of autologous ADMPC transplantation for periodontal tissue regeneration (2018)
  • Author(s): Chiaki Morimoto, Masahide Takedachi, Keigo Sawada, Satomi Yamamoto, Asae Hirai, Junpei Shimomura, Yuki Narukawa, Yoichiro Kashiwagi, Tomoaki Iwayama, Masahiro Kitamura, Shinya Murakami
  • Organizer: 第66回JADR総会・学術大会