| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
This study aimed to establish iPSCs from Runx2 homo-deficient (Runx2-/-) mouse and to elucidate the role of RUNX2 in the differentiation process into osteoblasts. Furthermore, we investigate the difference in the mechanisms involved in osteoblastic differentiation, in mouse iPSCs and human iPSCs. A later stage marker of osteogenesis were significantly lower in Runx2-/- miPSCs than in wild-type and Runx2+/- miPSCs, suggesting that osteoblastic differentiation will not proceed to later stages in Runx2-/- miPSCs. A total of 53 genes were downregulated and only three genes were upregulated in Runx2-/- miPSCs compared with wild-type miPSCs. We also confirmed that expression of genes by osteoblastic differentiation from human iPSCs suggesting that the function of Runx2 confirmed in the mouse model is the same in humans. Our finding will have implications for improving our understanding of RUNX2 functions in bone formation.
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