Development of a new treatment for obese diabetic periodontitis using anti-inflammatory miRNA-encapsulating exosomes
Project/Area Number |
18K17071
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 57030:Conservative dentistry-related
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Research Institution | Kyushu University |
Principal Investigator |
SANO TOMOMI 九州大学, 歯学研究院, 助教 (50782075)
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Project Period (FY) |
2018-04-01 – 2021-03-31
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Project Status |
Completed (Fiscal Year 2020)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 炎症 / 肥満 / 歯周病 / microRNA / 歯周炎 / 慢性炎症 / マクロファージ / 脂肪組織 / 歯周組織 / エクソソーム / インスリン抵抗性 |
Outline of Final Research Achievements |
In vitro study revealed that miR-146a transfection into either adipocytes or gingival fibroblasts, has resulted in a reduced cytokine gene expression, observed on co-culturing the cells with macrophages in the presence of LPS, in comparison to the control miRNA transfected. Similarly, miR-146a transfection into macrophages resulted in a reduced expression of TNF-α gene and protein in response to LPS stimulation. In vivo study revealed that a continuous intravenous miR-146a administration into mice via tail vein, protected the mice from developing obesity and the inflammatory cytokine gene expression was down-regulated in both adipose and periodontal tissues.
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Academic Significance and Societal Importance of the Research Achievements |
TNF-α中和抗体添加によりサイトカイン産生が抑制するという過去の結果から、今回用いた共培養系においてもmiR-146a-5pがマクロファージからのTNF-α発現を抑制し、結果的に炎症抑制につながったと考える。 miR-146a-5pは肥満糖尿病などにおいて脂肪・歯周組織の炎症に共通の制御因子として働く可能性がある。
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Anti-inflammatory Effects of miRNA-146a Induced in Adipose and Periodontal Tissues2020
Author(s)
Taiki Sanada, Tomomi Sano, Yusuke Sotomaru, Rehab Alshargabi, Yosuke Yamawaki, Akiko Yamashita, Hiroaki Matsunaga, Misaki Iwashita, Takanori Shinjo, Takashi Kanematsu, Tomoichiro Asano, Fusanori Nishimura
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Journal Title
Biochem Biophys Rep.
Volume: Jul
Pages: 100757-100757
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Dental pulp cell-derived powerful inducer of TNF-α comprises PKR containing stress granule rich microvesicles.2019
Author(s)
Suzuki S, Fukuda T, Nagayasu S, Nakanishi J, Yoshida K, Hirata-Tsuchiya S, Nakao Y, Sano T, Yamashita A, Yamada S, Ohta K, Shiba H, Nishimura F.
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Journal Title
Scientific Reports
Volume: 9
Issue: 1
Pages: 3825-3825
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Prolyl Isomerase Pin1 Suppresses Thermogenic Programs in Adipocytes by Promoting Degradation of Transcriptional Co-activator PRDM16.2019
Author(s)
Nakatsu Y, Matsunaga Y, Yamamotoya T, Ueda K, Inoue MK, Mizuno Y, Nakanishi M, Sano T, Yamawaki Y, Kushiyama A, Sakoda H, Fujishiro M, Ryo A, Ono H, Minamino T, Takahashi SI, Ohno H, Yoneda M, Takahashi K, Ishihara H, Katagiri H, Nishimura F, Kanematsu T, Yamada T, Asano T.
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Journal Title
Cell Rep.
Volume: 26
Issue: 12
Pages: 3221-3230
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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