Development of new bone regeneration therapy using Cluster of DFATs/ECM complex (C-DFAT)

• Project/Area Number: 18K17089
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57040:Regenerative dentistry and dental engineering-related
• Research Institution: Kagoshima University
• Principal Investigator: SHINOHARA Yukiya 鹿児島大学, 医歯学域歯学系, 助教 (30761647)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: DFAT / C-DFAT / 骨再生 / 歯周病 / BMP-9

Outline of Final Research Achievements

The purpose of this study was development of new bone regenerative therapy for lost bone tissues due to periodontal and oral disease by using Cluster of DFATs/ECM complex (C-DFAT).I confirmed the formation of C-DFAT which used extracelluar matrix (ECM) which rDFAT produced as a result of this study using rat DFAT(rDFAT) provided by ceiling culture.

Academic Significance and Societal Importance of the Research Achievements

組織幹細胞は加齢とともに減少していくことが知られており、組織内に存在する数も非常に少なくなる。中高年者や高齢者のQOLの維持・増進を考えた時、予知性の高い新規顎骨組織再生療法の開発は歯の保存や、理想的なインプラント治療の提供につながり、社会的見地からも極めて重要な課題である。そこで本研究の目的は、C-DFATの樹立、さらに顎顔面領域における歯周組織欠損、抜歯窩、顎骨再建などの骨欠損部に対して、scaffoldを用いずにC-DFATを細胞移植することで、安全・効率的かつ予知性の高い理想的な骨再生療法の基盤確立を目指すことであった。

Research Products

• [Journal Article] Comparison of periodontal wound healing/regeneration by recombinant human fibroblast growth factor-2 combined with β-tricalcium phosphate, carbonate apatite, or deproteinized bovine bone mineral in a canine 1-wall intrabony defect model . (2022)
  • Author(s): Yoshinori Shirakata, Fumiaki Setoguchi, Kotaro Sena, Toshiaki Nakamura, Takatomo Imafuji, Yukiya Shinohara, Masayuki Iwata, Kazuyuki Noguchi.
  • Journal Title: Journal of Clinical Periodontology Volume: ー
  • Peer Reviewed
• [Journal Article] Healing of buccal gingival recessions following treatment with coronally advanced flap alone or combined with a cross-linked hyaluronic acid gel. An experimental study in dogs (2021)
  • Author(s): Shirakata Y, Nakamura T, Kawakami Y, Imafuji T, Shinohara Y, Noguchi K, Sculean A
  • Journal Title: Journal of Clinical Periodontology Volume: 48 Issue: 4 Pages: 570-580
  • DOI: 10.1111/jcpe.13433
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Periodontal wound healing/regeneration of two-wall intrabony defects following reconstructive surgery with cross-linked hyaluronic acid-gel with or without a collagen matrix. A preclinical study in dogs. (2021)
  • Author(s): Yoshinori Shirakata, Takatomo Imafuji, Toshiaki Nakamura, Yoshiko Kawakami, Yukiya Shinohara, Kazuyuki Noguchi, Andrea Pilloni, Anton Sculean .
  • Journal Title: Quintessence International Volume: - Pages: 308-316
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Split-mouth evaluation of connective tissue graft with or withoutenamel matrix derivative for the treatment of isolated gingivalrecession defects in dogs (2018)
  • Author(s): Y. Shirakata, T. Nakamura, Y. Shinohara, K. Nakamura-Hasegawa, C. Hashiguchi, N. Takeuchi, T. Imafuji, A. Sculean, K. Noguchi
  • Journal Title: Clinical Oral Investigations Volume: - Issue: 8 Pages: 3339-3349
  • DOI: 10.1007/s00784-018-2750-1
  • Peer Reviewed
• [Presentation] Effects of hyaluronic acid on healing of gingival recession defects in dogs. (2020)
  • Author(s): Yoshinori Shirakata, Yukiya Shinohara .
  • Organizer: 106th Annual Meeting American Academy of Periodontology
  • Int'l Joint Research
• [Presentation] LIPUS enhances rhBMP9-induced bone formation in rat calvarial bone defects . (2020)
  • Author(s): Takatomo Imauji, Yoshinori Shirakata, Yukiya Shinohara .
  • Organizer: 106th Annual Meeting American Academy of Periodontology
  • Int'l Joint Research
• [Presentation] リコンビナントヒトBMP-9とLIPUS照射がラット頭蓋骨欠損の骨再生に及ぼす影響 (2019)
  • Author(s): 今藤隆智, 白方良典, 篠原敬哉, 中村利明, 野口和行
  • Organizer: 第62回秋季日本歯周病学会学術大会
• [Presentation] ラット頭蓋骨欠損モデルにおけるリコンビナントヒトBMP-9と低出力超音波パルス照射による骨再生効果 (2019)
  • Author(s): 今藤隆智, 白方良典, 篠原敬哉, 中村利明, 野口和行
  • Organizer: 令和元年度 日本歯周病学会九州五大学日本臨床歯周病学会九州支部合同研修会
• [Presentation] Healing of gingival recessions treated with coronally advanced flaps and subepithelial connective tissue grafts with or without enamel matrix derivative: A comparative study in dogs (2018)
  • Author(s): Y. Shirakata, T. Nakamura, Y. Shinohara, K. Nakamura-Hasegawa, C. Hashiguchi, N. Takeuchi, T. Imafuji, A. Sculean, K. Noguchi
  • Organizer: Europerio 9
  • Int'l Joint Research