New development of the cell control functional membrane

• Project/Area Number: 18K17122
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57050:Prosthodontics-related
• Research Institution: Hiroshima University
• Principal Investigator: Oki Yoshifumi 広島大学, 医系科学研究科(歯), 助教 (80806571)
• Project Period (FY): 2018-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 骨再生 / チタンメンブレン / アルカリ処理 / チタン薄膜 / インプラント / GBR

Outline of Final Research Achievements

Titanium membrane is often used for guided bone regeneration, but titanium has no osteoinductive ability. Therefore, various modification methods have been applied to modify titanium. The Purpose of this study was to evaluate the bioactive effect of titanium membrane. Titanium membranes were washed with acetone and distilled water (Control group). Then, the membranes were soaked in 5 N NaOH solution at 60 °C for 24 h (Alkali group). The samples were soaked in simulated body fluid. Deposition object was observed, and the compositions were analyzed. In vivo experiment, the critical-sized bone defect was prepared in calvaria of male Sprague-Dawley rat, and covered with alkali treated and control membrane, or without membrane. Tissue blocks were harvested at 4 weeks later, and bone tissue area ratio and bone membrane contact ratio were measured.
Alkali treated titanium membranes exhibited a high apatite-forming ability and high bone forming ability.

Academic Significance and Societal Importance of the Research Achievements

本研究のような生体活性を持つチタンメンブレンは，チタンメンブレンの持つ高い機械的強度のため，広範囲における骨欠損において高い空間保持能を持つ．また，チタンに対しては多くの表面処理が検討されており，申請者の先行研究においても，チタン薄膜構造体に対するアルカリ処理は骨形成を促進することが明らかになっている。これらのことから，本研究で開発されたチタンメンブレンは，骨再生療法に用いる生体材料として有用であることが期待できる．

Research Products

• [Journal Article] Development of a novel bioactive titanium membrane with alkali treatment for bone regeneration (2020)
  • Author(s): Hanako Umehara, Kazuya Doi, Yoshifumi Oki, Reiko Kobatake, Yusuke Makihara, Takayasu Kubo, Kazuhiro Tsuga.
  • Journal Title: Dental materials Journal Volume: -
  • NAID: 130007911289
  • Peer Reviewed
• [Journal Article] Effect of Autoclave heat sterilization on the surface properties of the sodium titaniate hydrogel layer on the alkali-treated titanium membrane (2019)
  • Author(s): Hanako Umehara, Kazuya Doi, Reiko Kobatake, Yoshifumi Oki, Yusuke Makihara, Takayasu Kubo, Kazuhiro Tsuga.
  • Journal Title: 広大歯誌 Volume: 51 Pages: 85-90
  • Peer Reviewed
• [Presentation] 骨形成を促進する生体活性チタン薄膜の開発 (2019)
  • Author(s): 梅原華子, 久保隆靖, 土井一矢, 小畠玲子, 沖 佳史, 牧原勇介, 津賀一弘
  • Organizer: 第49回公益社団法人日本口腔インプラント学会学術大会
• [Presentation] Novel bioactive titanium membrane for bone regeneration; effect of alkali treatment. (2019)
  • Author(s): Hanako Umehara, Kazuya Doi, Reiko Kobatake, Yusuke Makihara, Yoshifumi Oki, Takayasu Kubo, Kazuhiro Tsuga.
  • Organizer: European Association for Osseointegration 28th Annual Scientific Meeting
• [Presentation] The bioactive effect of alkali treated titanium membrane. (2019)
  • Author(s): Hanako Umehara, Kazuya Doi, Reiko Kobatake, Yoshifumi Oki, Yusuke Makihara, Takayasu Kubo, Kazuhiro Tsuga.
  • Organizer: DENTISPHERE 4-International Scientific Meeting, 5th Asean plus, Tokushima joint international conference