| Project/Area Number |
18K17128
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 57050:Prosthodontics-related
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| Research Institution | Showa University |
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Principal Investigator |
Suzuki Wataru 昭和大学, 歯学部, 兼任講師 (10736680)
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2021: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | シグナル伝達 / chondrocytes / 細胞内シグナル伝達 / 骨代謝 / Cdc42 / 骨・軟骨 |
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| Outline of Final Research Achievements |
The Rho family small G protein Cdc42 plays an important role in the body as a molecular switch that converts extracellular signals into cells. So far, it has been suggested that it has important functions in controlling cell skeleton, cell proliferation / death, and cell differentiation in vitro, and in vivo, especially in hard tissues. Studies of conditional knockout mice have suggested that it is an essential factor in cartilage formation. In this research project, the gene expression profilings using Cdc42 inhibitors, siRNAs, and Rho family protein activators have performed in order to investigate detailed functional analysis of Cdc42 chondrocytes.
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| Academic Significance and Societal Importance of the Research Achievements |
これまで、軟骨形成におけるCdc42の機能を検討するために、Cdc42コンディショナルノックアウトマウスを用いた研究を行ってきた。本研究では、軟骨細胞において、Cdc42の機能を低下させた際に、軟骨細胞内での遺伝子発現様式にどのような変化が起こるか、詳細な解析を行った。その結果、多くの遺伝子に発現の変化が認められた。本研究課題で得られた、遺伝子発現の網羅的解析結果は、今後の軟骨細胞機能の研究に意義ある結果を提示できたと考えられる。
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