| Project/Area Number |
18K17142
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 57050:Prosthodontics-related
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| Research Institution | Niigata University |
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Principal Investigator |
Ida Takako 新潟大学, 医歯学総合病院, 医員 (60790285)
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| Project Period (FY) |
2018-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 歯根膜 / コラーゲン / 歯学 / 力学的刺激 / SPARC / DDR2 / コラーゲン線維 |
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| Outline of Final Research Achievements |
The effect of DDR2 inhibitor on collagen fiber formation has been studied using mouse periodontal ligament-derived cells. The periodontal ligament-derived cells were cultured in the presence of DDR2 inhibitor (AZ628), LOX gene expression and LOX protein enzyme activity were measured, and the maturity of the matrix was evaluated by Picrosirius red staining.
Since SPARC has a calcium binding site in its domain, the low calcium rat models were used. As a result of bone structure analysis using 3D images obtained by μCT, bone density was decreased in the cortical bone and cancellous bone of the maxilla in the low calcium rat group compared with the normal diet group.
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| Academic Significance and Societal Importance of the Research Achievements |
力学的刺激による歯根膜線維の成熟を司る分子機構の解明は、患者個人によって異なる、力に対する応答の多様性を理解するために重要な知見となる可能性がある。本研究に期待される成果は、力学的刺激による歯根膜恒常性維持機構の解明という観点から歯科医学に貢献するだけでなく、コラーゲンレセプターによる力学的刺激の伝達機構の解明という観点から生命科学の発展に寄与する。
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