Development of alveolar bone regeneration with platelet-rich plasma using a novel double-activation method

• Project/Area Number: 18K17160
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57050:Prosthodontics-related
• Research Institution: Osaka Dental University
• Principal Investigator: Hara Tomoya 大阪歯科大学, 歯学部, 講師 (60782092)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 多血小板血漿 / PRP / Platelet-Rich Plasma / 歯槽骨再生 / 電解機能水 / 次亜塩素酸水 / 酸性電解水 / 電荷電位水 / 酸化電位水

Outline of Final Research Achievements

Platelet-rich plasma (PRP) is a plasma containing a large amount of growth factors regulated from autologous blood and is effective in promoting bone regeneration and wound healing. On the other hand, there have been reports that the effects of PRP are limited and clinically unstable. We believe that this is due to the low amount of growth factors in PRP and have devised a novel double-activation method. This method increases the amount of growth factors in PRP by adding electrolyzed water to the PRP that has already been adjusted by the existing PRP adjustment method. This study aims to enhance the effect of PRP and to achieve rapid and reliable alveolar bone regeneration by molecular biological evaluation of the change in the amount of growth factors by the novel double-activation method.

Academic Significance and Societal Importance of the Research Achievements

近年、骨欠損に対して骨再生誘導法等で骨再生を試みインプラント治療を行っているが決定的な方法とは言い難い。そのため、画期的な骨再生治療を開発し迅速かつ確実な歯槽骨再生を可能にすれば、高齢化社会の質的向上へ大きく寄与できる。PRPは自己全血から調整されるもので骨再生、創傷治癒促進効果等を期待されすでに臨床応用されている。この効果は血小板内の増殖因子より起こる。しかし、多血小板血漿による骨再生効果は安定しないという問題が依然として残っている。今回、PRPの増殖因子量を増加させる方法として新規ダブルアクチべート法を考案した。このことにより、迅速かつ確実な歯槽骨再生が可能となる。

Research Products

• [Journal Article] Improvement of occlusal balance and quality of life in patients by restoration with dental implant (2020)
  • Author(s): Honda M, Yamada Y, Nakamura-Yamada S, Hara T, Tanioka T, Kusano K, Baba S
  • Journal Title: 日本口腔リハビリテーション学会雑誌 Volume: 33 Pages: 27-35
  • NAID: 40022496904
  • Peer Reviewed
• [Journal Article] Bone regenerative therapy in the aging society (2018)
  • Author(s): Hara Tomoya、Kakudo Natsuko、Morimoto Naoki、Kusumoto Kenji
  • Journal Title: The Journal of Kansai Medical University Volume: 69 Issue: 0 Pages: 19-22
  • DOI: 10.5361/jkmu.69.19
  • NAID: 130007553706
  • ISSN: 0022-8400, 2185-3851
  • Peer Reviewed / Open Access
• [Journal Article] Osteogenesis of Multipotent Progenitor Cells using the Epigallocatechin Gallate-Modified Gelatin Sponge Scaffold in the Rat Congenital Cleft-Jaw Model (2018)
  • Author(s): Sasayama Satoshi、Hara Tomoya、Tanaka Tomonari、Honda Yoshitomo、Baba Shunsuke
  • Journal Title: International Journal of Molecular Sciences Volume: 19 Issue: 12 Pages: 3803-3803
  • DOI: 10.3390/ijms19123803
  • Peer Reviewed / Open Access
• [Presentation] ラット先天性顎裂モデル内における多能性前駆細胞およびカテキン結合ゼラチンにて形成された再生組織 (2019)
  • Author(s): 笹山智史, 原朋也, 本多正明, 本田義知, 馬場俊輔
  • Organizer: 日本口腔インプラント学会第39回近畿・北陸支部学術大会
• [Presentation] 多能性前駆細胞を播種したEGCG結合ゼラチンのラット先天性顎裂内での骨形成挙動 (2019)
  • Author(s): 笹山智史, 原朋也, 田中知成, 本田義知, 馬場俊輔
  • Organizer: 第73回日本歯科理工学会学術講演会