Mechanism of lymph node stromal cells and immune tolerance in lymph node metastasis of oral cancer

• Project/Area Number: 18K17186
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57060:Surgical dentistry-related
• Research Institution: Hyogo Medical University
• Principal Investigator: Yoshikawa Kyohei 兵庫医科大学, 医学部, 助教 (00727480)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 口腔扁平上皮癌 / 頸部リンパ節転移 / エクソソーム / ストローマ細胞 / 免疫寛容 / リンパ節転移 / タンパク発現 / 扁平上皮癌細胞株 / FRCｓ

Outline of Final Research Achievements

We established cell lines from the dissected metastatic cervical lymph nodes of an oral squamous cell carcinoma patient. In recent years, the role of exosome secreted by cancer cells is noticed and we made a success for extracting it from oral squamous cell carcinoma cell line. Furthermore, high expressions of exosome marker were seen in western blot analyzing. From these results, functional analysis of exosome could be a new information for the mechanism of cancer metastasis and therapeutic target.

Academic Significance and Societal Importance of the Research Achievements

口腔癌では「頸部転移」が重要な予後因子であり、しばしば予後不良となる症例に遭遇することも多く、口腔癌の治療成績を向上させるためには、エクソソームの機能解析をさらにすすめることで、癌の増殖や浸潤、転移に対する新たな治療戦略を見いだすことができる可能性が高いと考えられる。

Research Products

• [Journal Article] A case of tongue carcinoma occurred in patient of Ehlers-Danlos syndrome (2021)
  • Author(s): 吉川恭平, 野口一馬, 山根木康嗣, 吉田和功, 森寺邦康, 高岡一樹, 岸本裕充
  • Journal Title: Journal of Japanese Society of Oral Oncology Volume: 33 Issue: 1 Pages: 29-34
  • DOI: 10.5843/jsot.33.29
  • NAID: 130008001885
  • ISSN: 0915-5988, 1884-4995
  • Peer Reviewed
• [Journal Article] Partial maxillectomy for upper gingival carcinoma with a cheek-splitting transbuccal approach and reconstruction by buccal fat pad flap: a case report (2021)
  • Author(s): Kyohei Yoshikawa, Kazuma Noguchi, Ayaka Hatanaka, Koji Yamanegi, Yuji Omori, Kazunari Yoshida, Shuji Kanda, Kuniyasu Moridera, Kazuki Takaoka, Hiromitsu Kishimoto
  • Journal Title: Oral Science in japan 2020 Volume: - Pages: 27-30
• [Journal Article] Occurrence of a mucocele in a submandibular gland containing a sialolith: A case report (2020)
  • Author(s): Kyohei Yoshikawa , Kazuma Noguchi, Koji Yamanegi, Masumi Nakanishi, Kazuki Takaoka, Hiromitsu Kishimoto
  • Journal Title: Oral Science International Volume: - Issue: 3 Pages: 209-212
  • DOI: 10.1002/osi2.1062
  • Peer Reviewed
• [Journal Article] 内側腓腹動脈穿通枝皮弁を用いた舌癌再建後の機能評価 (2020)
  • Author(s): 吉川恭平、野口一馬、 高岡一樹、 森寺邦康、石瀬久子、曽束洋平、西本 聡、垣淵正男、岸本裕充
  • Journal Title: 兵庫医科大学医学会雑誌 Volume: ４４ Pages: 107-111
  • Peer Reviewed
• [Journal Article] Bilateral incidence of central odontogenic fibroma simulating hyperplastic dental follicle: A case report (2018)
  • Author(s): kyohei Yoshikawa, Kazuma Noguchi, Koji Yamanegi, Kazuki Takaoka, Hiromitsu Kishimoto
  • Journal Title: Oral Science in Japan 2018 Volume: なし Pages: 57-60
• [Presentation] エーラス・ダンロス症候群患者に発生した舌癌の１例 (2020)
  • Author(s): 吉川恭平、野口一馬、吉田和功、中西満寿美、神田修治、森寺邦康、高岡一樹、岸本裕充
  • Organizer: 第38回日本口腔腫瘍学会総会・学術大会
• [Presentation] セツキシマブ併用放射線療法により重篤な間質性肺炎を発症した舌癌の１例 (2019)
  • Author(s): 吉川恭平, 野口一馬, 中西満寿美, 吉田和功, 高岡一樹, 岸本裕充
  • Organizer: 第64回公益社団法人 日本口腔外科学会総会・学術大会
• [Presentation] 両側下顎骨に発生した中心性歯原性線維腫の１例 (2018)
  • Author(s): 吉川恭平, 野口一馬, 森寺邦康, 高岡一樹, 岸本裕充
  • Organizer: 第72回NPO法人日本口腔科学会学術集会
• [Presentation] シスプラチン耐性口腔扁平上皮癌におけるHippo pathwayの関与と遺伝子解析 (2018)
  • Author(s): 吉川恭平, 野口一馬, 中西満寿美, 高岡一樹, 岸本裕充
  • Organizer: 第63回（公社）日本口腔外科学会総会・学術大会