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Analysis of soil inhabited by amoebae which causes meningoencephalitis and development of new cell-free culture method

Research Project

Project/Area Number 18K17349
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 58020:Hygiene and public health-related: including laboratory approach
Research InstitutionHirosaki University

Principal Investigator

Yamanouchi Kanako  弘前大学, 保健学研究科, 助教 (80740810)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsアメーバ性髄膜脳炎 / 土壌解析 / 無細胞培養 / 生息特性 / 無菌培養 / バラムチア / 自由生活性アメーバ / 培養 / 土壌 / Balamuthia mandrillaris / 青森
Outline of Final Research Achievements

Balamcia mandrilaris, which is classified as a free-living amoeba, infects humans and causes meningoencephalitis.Since almost 100% of the affected people of this amoeba die, there is an urgent need to establish an early diagnosis and treatment method. However, the existing balamcia culture method has a very complicated composition and low versatility, and is not particularly suitable for culturing environmental balamcia.Therefore, we aimed to clarify the habitat characteristics and develop a new culture method through the analysis of the soil in which Balamcia inhabits.Soil analysis revealed that Balamcia in the environment prefers eutrophic conditions and prefers mineral-containing alkaline soils.n the prototype culture solution, we succeeded in culturing environmental balamcia by reproducing the electrical conductivity close to that of soil.

Academic Significance and Societal Importance of the Research Achievements

本研究課題の自由生活性アメーバは、土壌、海水、室内塵などの生活環境下で分離され、世界中でその生息が確認されている。常に人の身近に存在してきた自由生活性アメーバは、長い間無害であると認識されてきた。しかし近年は、ヒトを含む哺乳動物に感染し髄膜脳炎、皮膚アメーバ症や角膜炎などを引き起こすことが確認され、臨床例が世界各国で報告されている。本研究により環境株、臨床株ともに簡便に培養することが可能となれば、アメーバの生態研究や新たな治療・検査法を構築するための研究が活発化すると思われる。世界的に停滞を示す致死性アメーバ感染症研究において、新規培養法の構築は学術的・社会的に意義のある研究と思われる。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (4 results)

All 2019 2018

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (2 results)

  • [Journal Article] First report of the isolation of Balamuthia mandrillaris in the northern region of Japan2018

    • Author(s)
      Yamanouchi K, Arima H, Sakamoto Y, Kanto K, Kasai K, Ito K, Inaba T
    • Journal Title

      Parasitology research

      Volume: 117 Issue: 9 Pages: 2895-2900

    • DOI

      10.1007/s00436-018-5980-x

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of a method to extract protozoan DNA from black soil2018

    • Author(s)
      KanakoYamanouchi, MasahiroTakeuchi, HiroakiArima, TakakiyoTsujiguchi
    • Journal Title

      Parasite Epidemiol Control

      Volume: 3 Pages: e00081-e00081

    • DOI

      10.1016/j.parepi.2018.e00081

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 自由生活性アメーバ Balamuthia mandrillaris. は新たな病原体ベクターか?2019

    • Author(s)
      山内可南子、辻口貴清、坂本瑞生
    • Organizer
      第78回日本公衆衛生学会総会
    • Related Report
      2019 Research-status Report
  • [Presentation] 環境由来 Balamuthia mandrillaris に内在する細菌の同定2018

    • Author(s)
      齋藤真帆,山屋理樹,坂本倭,山内可南子
    • Organizer
      平成30年度 日臨技北日本支部医学検査学会(第7回)
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2023-01-30  

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