リン脂質代謝調節に基づくサルコペニアの新規分子機序の解明
Project/Area Number |
18K17935
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 59040:Nutrition science and health science-related
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Research Institution | Keio University (2020) Juntendo University (2018-2019) |
Principal Investigator |
門口 智泰 慶應義塾大学, 医学部(信濃町), 研究員 (10762049)
|
Project Period (FY) |
2018-04-01 – 2021-03-31
|
Project Status |
Discontinued (Fiscal Year 2020)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | 筋萎縮 / サルコペニア / リン脂質 / 加齢 / 老化 / ドコサヘキサエン酸 / 心不全 |
Outline of Annual Research Achievements |
令和2年度は、6ヶ月齢の若齢マウスおよび24ヶ月齢に到達した加齢マウスの下肢骨格筋サンプル(腓腹筋)を用い、リピドーム解析によりリン脂質代謝産物を網羅的に観察することで加齢による筋萎縮とリン脂質代謝産物との関連性を調べた。 各群の下肢骨格筋サンプルを対象に、エレクトロスプレーイオン化法を用いた液体クロマトグラフィータンデム質量分析によるエイコサノイド・ドコサノイド解析を実施し、リン脂質代謝産物を同定した。大変興味深いことに、一部のアラキドン酸代謝産物、エイコサペンタエン(EPA)酸代謝産物、ドコサヘキサエン酸(DHA)代謝産物が加齢マウスの萎縮筋において有意に増加し、リノール酸代謝産物においては低下傾向を認めた。中でもEPA代謝産物は加齢マウスの萎縮筋において、より大きな変化を認めていた。このことから、萎縮筋におけるリン脂質代謝、特にEPA代謝異常が加齢に伴う筋萎縮において重要な役割を果たしている可能性が考えられる。 これら代謝産物の変化がどのように起きているのかを明らかにするため、今後は主要なリン脂質代謝酵素であるリゾフォスファチジン酸アシル基転移酵素およびリゾフォスファチジルコリンアシル基転移酵素などとの関連性について検討する必要がある。
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Report
(3 results)
Research Products
(24 results)
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[Journal Article] Association of advanced glycation end products with sarcopenia and frailty in chronic kidney disease.2020
Author(s)
Yabuuchi、J., Ueda, S., Yamagishi, S-I, Nohara, N., Nagasawa, H., Wakabayashi, K., Matsui, T., Higashimoto Y., Kadoguchi, T., Otsuka, T., Gohda, T., Suzuki, Y.
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Journal Title
Sci. Rep.
Volume: 10
Issue: 1
Pages: 17647-17659
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Enhanced monocyte migratory activity in the pathogenesis of structural remodeling in atrial fibrillation2020
Author(s)
Katsutoshi Miyosawa, Hiroshi Iwata, Asuka Minami-Takano, Hidemori Hayashi, Haruna Tabuchi, Gaku Sekita, Tomoyasu Kadoguchi, Kai Ishii, Yui Nozaki, Takehiro Funamizu, Yuichi Chikata, Satoshi Matsushita, Atsushi Amano, Masataka Sumiyoshi, Yuji Nakazato, Hiroyuki Daida, Tohru Minamino
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Journal Title
PLoS ONE
Volume: 15
Issue: 10
Pages: 1-16
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Promotion of oxidative stress is associated with mitochondrial dysfunction and muscle atrophy in aging mice.2019
Author(s)
Kadoguchi T, Shimada K, Miyazaki T, Kitamura K, Kunimoto M, Aikawa T, Sugita Y, Ouchi S, Shiozawa T, Yokoyama-Nishitani M, Fukao K, Miyosawa K, Isoda K, Daida H.
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Journal Title
Geriatr Gerontol Int.
Volume: 20
Pages: 78-84
Related Report
Peer Reviewed / Open Access
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[Journal Article] Associations among circulating levels of follistatin-like 1, clinical parameters, and cardiovascular events in patients undergoing elective percutaneous coronary intervention with drug-eluting stents.2019
Author(s)
Aikawa T, Shimada K, Miyauchi K, Miyazaki T, Sai E, Ouchi S, Kadoguchi T, Kunimoto M, Joki Y, Dohi T, Okazaki S, Isoda K, Ohashi K, Murohara T, Ouchi N, Daida H.
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Journal Title
Related Report
Peer Reviewed / Open Access
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[Journal Article] Relationship between the Kihon Checklist and the clinical parameters in patients who participated in cardiac rehabilitation.2019
Author(s)
Kunimoto M, Shimada K, Yokoyama M, Matsubara T, Aikawa T, Ouchi S, Shimizu M, Fukao K, Miyazaki T, Kadoguchi T, Fujiwara K, Honzawa A, Yamada M, Shimada A, Yamamoto T, Amano A, Daida H.
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Journal Title
Geriatr Gerontol Int.
Volume: 19
Pages: 287-292
Related Report
Peer Reviewed / Open Access
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[Journal Article] Inhibition of interleukin-1 suppresses angiotensin II-induced aortic inflammation and aneurysm formation.2018
Author(s)
Isoda K, Akita K, Kitamura K, Sato-Okabayashi Y, Kadoguchi T, Isobe S, Ohtomo F, Sano M, Shimada K, Iwakura Y, Daida H
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Journal Title
Int J Cardiol
Volume: 270
Pages: 221-227
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Levels of phospholipids and triacylglycerol-containing omega 3 fatty acids in myocardial tissue of patients with myocardial infarction: analyzed by a lipidomics profiling method2020
Author(s)
Shiozawa T, Shimada K, Lee HC, Kadoguchi T, Aikawa T, Hayashi H, Miyazaki T, Matsushita S, Suwa S, Yokomizo T, Amano A, Nakazato Y, Daida H
Organizer
European society of cardiology
Related Report
Int'l Joint Research
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[Presentation] Relationship between skin autofluorescence levels and clinical outcomes in heart failure patients undergoing cardiac rehabilitation2019
Author(s)
Kunimoto M, Shimada K, Yokoyama M, Honzawa A, Yamada M, Matsubara T, Fukao K, Kadoguchi T, Fujiwara K, Miyazaki T, Yamamoto T, Takahashi T, Fujiwara T, Amano A, Daida H.
Organizer
European Society of Congress 2019
Related Report
Int'l Joint Research
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[Presentation] Voluntary exercise associated with myokine production ameliorates cardiac remodeling and inflammation in a myocardial infarction mouse model.2019
Author(s)
Kadoguchi T, Shimada K, Hamad A, Aikawa T, Ouchi S, Kitamura K, Kunimoto M, Fukao K, Yokoyama M, Sugita Y, Shiozawa T, Matsushita S, Miyazaki T, Isoda K, Daida H.
Organizer
European Society of Congress 2019
Related Report
Int'l Joint Research
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[Presentation] Prognostic Impact of Peak Oxygen Uptake and Heart Rate Reserve in Patients After Off-pump Coronary Artery Bypass Grafting2019
Author(s)
Abulimiti A, Shimada K, Yokoyama M, Kunimoto M, Matsubara T, Fujiwara K, Aikawa T, Ouchi S, Shimizu M, Fukao F, Kadoguchi T, Miyazaki T, Shimada A, Yamamoto T, Takahashi T, Fujiwara T, Amano A, Daida H.
Organizer
European Society of Congress 2019
Related Report
Int'l Joint Research
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[Presentation] Angiotensin II-dependent Activation of NADPH Oxidase 4 Contributes to Muscle Wasting in Mice via Downregulation of NF-E2-related Factor 2.2018
Author(s)
Kadoguchi T, Shimada K, Miyazaki T, Aikawa T, Ouchi S, Kitamura K, Kunimoto M, Sugita Y, Shiozawa T, Isoda K, Daida H
Organizer
European Society of Congress 2018
Related Report
Int'l Joint Research
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[Presentation] Malnutrition and Low Omega 6 PUFA Levels on Admission Affect the Development of Delirium in Patients With Acute Cardiovascular Disease Admitted to Coronary Care Unit2018
Author(s)
Sugita Y, Miyazaki T, Shimada K, Shimizu M, Kunimoto M, Aikawa T, Ouchi S, Kadoguchi T, Kawaguchi Y, Shiozawa T, Takasu K, Hiki M, Takahashi S, Yokoyama M, Iwata H, Daida H
Organizer
American Heart Association Scientific Sessions 2018
Related Report
Int'l Joint Research
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[Presentation] アンジオテンシンIIによる筋萎縮におけるNADPH oxidaseの働き2018
Author(s)
門口智泰, 島田和典, 小出 寛, 宮崎哲朗,相川達郎, 大内翔平, 塩澤知之, 高橋秀平, 喜多村健一, 國本充洋, 杉田有里那, 秋田耕嗣, 磯田菊生, 代田浩之
Organizer
第6回骨格筋生物学研究会
Related Report