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Polymer nano-complex-based high efficient control of the dynamics of living membranes and the application

Research Project

Project/Area Number 18K18384
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 90120:Biomaterials-related
Research InstitutionThe University of Tokyo (2019)
Tokyo Institute of Technology (2018)

Principal Investigator

Masuda Tsukuru  東京大学, 大学院工学系研究科(工学部), 助教 (70814010)

Project Period (FY) 2018-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords生体材料 / 高分子材料 / 生体膜活性化ペプチド / 精密ラジカル重合 / 生体膜 / 生体膜透過ペプチド / 膜破壊ペプチド / 刺激応答性高分子
Outline of Final Research Achievements

In this study, the nano-complex of the smart polymer and membrane-active peptide, E5 has been designed to control the transformation of lipid membranes. A cationic comb-type polymer composed of the cationic main chain and hydrophilic side chain forms a complex with E5 peptide, resulting in enhancement the activity of E5. When this complex was added to the liposome, transformation from vesicle into sheet was induced. This would be because the complex significantly decreased the interfacial energy of the lipid membrane. Moreover, A cationic comb-type copolymer with thermoresponsive side chain was designed. The use of the thermoresponsive polymer allowed the transformation control of the lipid membrane by temperature change. A novel method to modifying the polymer chain onto the surface of lipid membrane was also developed.

Academic Significance and Societal Importance of the Research Achievements

生体膜の形態変化・出芽・融合といった動的な挙動を生理的な条件下において効率的に制御する人工材料が設計できれば、生体膜の動的挙動の機構解明や新規な薬物送達など生体材料としての応用に大きく貢献することができる。
本高分子・ペプチド複合体が誘起する脂質膜のベシクル(小胞)からシート形状への構造転移は100%近い高効率である点が特徴のひとつである。さらに、刺激応答性高分子の相転移とカップリングした構造転移の制御にも成功している。このような環境変化を認識した脂質膜の構造転移や、本研究で開発した効果的に高分子材料と生体分子を複合化する手法は、新しい生体材料としての展開が期待される。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (18 results)

All 2020 2019 2018 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (13 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Cationic Copolymer‐Chaperoned 2D?3D Reversible Conversion of Lipid Membranes2019

    • Author(s)
      Shimada Naohiko、Kinoshita Hirotaka、Umegae Takuma、Azumai Satomi、Kume Nozomi、Ochiai Takuro、Takenaka Tomoka、Sakamoto Wakako、Yamada Takayoshi、Furuta Tadaomi、Masuda Tsukuru、Sakurai Minoru、Higuchi Hideo、Maruyama Atsushi
    • Journal Title

      Advanced Materials

      Volume: 31 Issue: 44 Pages: 1904032-1904032

    • DOI

      10.1002/adma.201904032

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Liposome-Surface-Initiated ARGET ATRP: Surface Softness Generated by “Grafting from” Polymerization2019

    • Author(s)
      Masuda Tsukuru, Shimada Naohiko, Maruyama Atsushi
    • Journal Title

      Langmuir

      Volume: 35 Issue: 16 Pages: 5581-5586

    • DOI

      10.1021/acs.langmuir.9b00450

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cationic Copolymers Act As Chaperones of a Membrane-Active Peptide: Influence on Membrane Selectivity2019

    • Author(s)
      Sakamoto Wakako, Masuda Tsukuru, Ochiai Takuro, Shimada Naohiko, Maruyama Atsushi
    • Journal Title

      ACS Biomaterials Science & Engineering

      Volume: 印刷中 Issue: 11 Pages: 5744-5751

    • DOI

      10.1021/acsbiomaterials.8b01582

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] Sheet/vesicle transformation of lipid membranes by thermoresponsive cationic comb-type copolymer/peptide complexes2019

    • Author(s)
      増田造・落合拓郎・嶋田直彦・丸山厚
    • Organizer
      第68回 高分子学会年次大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] リポソーム表面開始ATRP:”grafting from”法によるリポソーム表面への高分子修飾2019

    • Author(s)
      増田造・嶋田直彦・丸山厚
    • Organizer
      第68回 高分子学会年次大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] リポソーム表面開始ATRP:”Grafting from”法によるソフトな高分子修飾表面を持つリポソームの調製2019

    • Author(s)
      増田造・嶋田直彦・丸山厚
    • Organizer
      第68回 高分子討論会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 高分子の精密重合法を利用した生体分子修飾及び生体材料設計2019

    • Author(s)
      増田造
    • Organizer
      次世代医療技術研究会 2019年 第3回情報交換会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] リポソーム表面開始精密ラジカル重合: ”grafting from”法によるソフトな高分子表面を持つリポソームの調製2019

    • Author(s)
      増田造・嶋田直彦・丸山厚
    • Organizer
      第29回 MRS-J年次大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 自律応答型脂質デバイスの創製:高分子/ペプチド複合体による生体膜形態操作2019

    • Author(s)
      増田造・落合拓郎・嶋田直彦・丸山厚
    • Organizer
      第28回 インテリジェント・ナノ材料シンポジウム
    • Related Report
      2018 Research-status Report
  • [Presentation] 自律応答型脂質デバイスの創製:温度応答型カチオン性くし形共重合体/ペプチド複合体による生体膜形態操作2019

    • Author(s)
      増田造・落合拓郎・嶋田直彦・丸山厚
    • Organizer
      第30回 高分子ゲル研究討論会
    • Related Report
      2018 Research-status Report
  • [Presentation] 自律応答型脂質デバイスの創製:温度応答型カチオン性くし形共重合体/ペプチド複合体による脂質膜形態の操作2018

    • Author(s)
      増田造・落合拓郎・嶋田直彦・丸山厚
    • Organizer
      第67回 高分子討論会
    • Related Report
      2018 Research-status Report
  • [Presentation] 温度応答型カチオン性くし型共重合体/ペプチド複合体による脂質膜形態の操作2018

    • Author(s)
      増田造・落合拓郎・嶋田直彦・丸山厚
    • Organizer
      第40回 バイオマテリアル学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 温度応答型カチオン性くし型共重合体/ペプチド複合体による脂質膜のシート・ベシクル転移制御2018

    • Author(s)
      増田造・落合拓郎・嶋田直彦・丸山厚
    • Organizer
      第28回 日本MRS年次大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Smart lipid membrane devices: Control of lipid membrane morphology by thermoresponsive cationic comb-type copolymer/peptide complex2018

    • Author(s)
      Masuda Tsukuru, Ochiai Takuro, Shimada Naohiko, Maruyama Atsushi
    • Organizer
      International Polymer Conference 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Smart lipid membrane devices: Lipid membrane morphology control by a thermoresponsive cationic comb-type copolymer/peptide nano-assembly2018

    • Author(s)
      Masuda Tsukuru, Ochiai Takuro, Shimada Naohiko, Maruyama Atsushi
    • Organizer
      1st Glowing Polymer Symposium in KANTO
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Vesicle-to-sheet Transition of Copolymer Modified Liposomes with Membrane Disruptive Peptide2018

    • Author(s)
      Takenaka Tomoka, Ochiai Takuro, Sakamoto Wakako, Masuda Tsukuru, Shimada Naohiko, Maruyama Atsushi
    • Organizer
      10th International Peptide Symposium
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Book] 月刊 機能材料 (2020年5月号 スマートポリマーの最新展望)2020

    • Author(s)
      増田造・坂本和歌子・嶋田直彦・丸山厚
    • Publisher
      シーエムシー出版
    • Related Report
      2019 Annual Research Report
  • [Remarks] 人工シャペロンにより脂質二重膜の2次元/3次元構造の高効率で可逆的な変換に初めて成功

    • URL

      https://www.titech.ac.jp/news/2019/045898.html

    • Related Report
      2019 Annual Research Report

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Published: 2018-04-23   Modified: 2021-02-19  

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