Experimental study of levitating micro-organisms in plasma and its application

• Project/Area Number: 18K18750
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 14:Plasma science and related fields
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: Sanpei Akio 京都工芸繊維大学, 電気電子工学系, 准教授 (90379066)
• Project Period (FY): 2018-06-29 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 微粒子プラズマ / プラズマ応用 / クーロン結晶 / 微生物 / 殺菌滅菌

Outline of Final Research Achievements

This study reports levitation of microorganisms in the sheath of radio-frequency (RF) plasma. A crystal-like structure was successfully formed as a result of the levitation of a kind of bacillus micro-organisms Klebsiella pneumoniae in a plasma. An external electric field was applied to the plasma. The experimental results revealed that the behavior of the levitated microorganisms differs depending on the species.

Academic Significance and Societal Importance of the Research Achievements

プラズマ中に微粒子を入れると負に帯電し，クーロン力や重力等の力が釣り合う位置で浮遊する事実は良く知られている．本研究では低温プラズマ中に種々の細菌を導入し，それらが帯電することでプラズマに浮遊することを確認され，自発的に秩序構造が形成されることを観測した．この事実は機能性材料・アストロバイオロジー・医療分野に対する応用へ期待できる．

Research Products

• [Journal Article] Collection of microorganisms levitating in a radio-frequency discharge (2021)
  • Author(s): Kawade Yasutaka、Sanpei Akio、Akamatsu Keita、Ochiai Ryotaro、Okada Toshikazu、Hayashi Yasuaki、Himura Haruhiko
  • Journal Title: Japanese Journal of Applied Physics Volume: 60 Issue: 4 Pages: 046002-046002
  • DOI: 10.35848/1347-4065/abe643
  • Peer Reviewed
• [Journal Article] Removal of ghost particles from the reconstruction of dusty plasma in integral photography by three-dimensional deconvolution (2020)
  • Author(s): Sanpei Akio、Kai Eisaku、Kawade Yasutaka
  • Journal Title: Optics Express Volume: 28 Issue: 25 Pages: 37743-37743
  • DOI: 10.1364/oe.409139
  • Peer Reviewed / Open Access
• [Journal Article] Controlling the Diameter of a Pure Electron Plasma to Produce an Exact Two-Fluid Plasma State in a Nested Trap (2019)
  • Author(s): KATO Toshiki、HIMURA Haruhiko、SOWA Shinji、SANPEI Akio
  • Journal Title: Plasma and Fusion Research Volume: 14 Issue: 0 Pages: 1201039-1201039
  • DOI: 10.1585/pfr.14.1201039
  • NAID: 130007649947
  • ISSN: 1880-6821
  • Year and Date: 2019-02-25
  • Peer Reviewed / Open Access
• [Journal Article] First Observation of Crystallike Configuration of Microorganisms in an RF Plasma (2019)
  • Author(s): Sanpei Akio、Kigami Tomohito、Hayashi Yasuaki、Himura Haruhiko、Masamune Sadao、Sampei Mai
  • Journal Title: IEEE Transactions on Plasma Science Volume: 47 Issue: 7 Pages: 3074-3078
  • DOI: 10.1109/tps.2019.2916422
  • Peer Reviewed
• [Presentation] Three-dimensional imaging diagnostics for plasmas with integral photography and deconvolution techniques (2020)
  • Author(s): A. Sanpei, E. Kai, H. Himura, Y. Hayashi, S. Masamune, S. Ohdachi, K. Nagasaki
  • Organizer: OSA Imaging and Applied Optics Congress
  • Int'l Joint Research / Invited
• [Presentation] RFプラズマ中の微生物および非球形微粒子の浮遊実験 (2020)
  • Author(s): 三瓶明希夫， 川出恭隆，赤松慧汰，落合遼太朗，比村治彦，林康明
  • Organizer: 第81回応用物理学会
• [Presentation] Observation of Microorganisms Levitation Phenomenon in an RF Plasma (2020)
  • Author(s): Y. Kawade, A. Sanpei, K. Akamatsu, R. Ochiai, Y. Hayashi, H. Himura
  • Organizer: The 29th International Toki Conference
  • Int'l Joint Research
• [Presentation] Comparative observation of the behavior of various microorganisms levitate in an RF plasma (2020)
  • Author(s): R. OCHIAI, Y. KAWADE, A. SANPEI, I. YONEDA, K. AKAMATSU, Y. HAYASHI, H. HIMURA
  • Organizer: The 21st Workshop on Fine Particle Plasmas
  • Int'l Joint Research
• [Presentation] Removal of ghost particles from the reconstruction of dusty plasma in integral photography by three-dimensional deconvolution (2020)
  • Author(s): Akio Sanpei, Y. Kawade, K. Akamatsu, R. Ochiai, Y. Hayashi
  • Organizer: The 21st Workshop on Fine Particle Plasmas
  • Int'l Joint Research
• [Presentation] Influence of external field on levitating microorganisms in an RF plasma (2020)
  • Author(s): 川出恭隆，三瓶明希夫
  • Organizer: ISPlasma2020
  • Int'l Joint Research
• [Presentation] RFプラズマ空間中における微生物浮遊現象に関する研究 (2019)
  • Author(s): 川出恭隆，三瓶明希夫
  • Organizer: 第80回応用物理学会
• [Presentation] RFプラズマ中のロッド状微粒子についての挙動シミュレーション (2019)
  • Author(s): 赤松慧太，三瓶明希夫
  • Organizer: 第36回 プラズマ・核融合学会 年会
• [Presentation] RFプラズマ中における細菌浮遊現象に対する挙動の菌種依存性の研究 (2019)
  • Author(s): 川出恭隆，三瓶明希夫
  • Organizer: 第36回 プラズマ・核融合学会 年会
• [Presentation] Effects of external fields on levitating microorganisms in an RF plasma (2019)
  • Author(s): 川出恭隆，三瓶明希夫
  • Organizer: 20TH WORKSHOP ON FINE PARTICLE PLASMAS
  • Int'l Joint Research
• [Presentation] Three-dimensional imaging of levitating dust and background plasma through integral photography with deconvolution (2019)
  • Author(s): 三瓶明希夫
  • Organizer: 20TH WORKSHOP ON FINE PARTICLE PLASMAS
  • Int'l Joint Research
• [Presentation] Levitation Phenomena of Microorganisms in Ionized Gas (2019)
  • Author(s): Akio Sanpei, Tomohito Kigami, Itaru Yoneda, Yasutaka Kawade, Yasuaki Hayashi, Haruhiko Himura, Sadao Masamune and Mai Sampei
  • Organizer: The 3rd International Symposium Kyoto Biomolecular Mass Spectrometry Society
  • Int'l Joint Research
• [Presentation] RFプラズマ中の微生物浮遊実験 (2019)
  • Author(s): 三瓶明希夫，木上智仁，米田至，川出恭隆，林康明，比村治彦，政宗貞男，三瓶舞
  • Organizer: 第66回応用物理学会春季学術講演会
• [Presentation] RFプラズマ中の微生物の浮遊とクーロン結晶形成 (2018)
  • Author(s): 三瓶明希夫
  • Organizer: H30 年度 東北大学電気通信研究所 共同プロジェクト研究会 微粒子-流体混成系がもたらす多様な機能性
  • Invited
• [Presentation] Levitation of micro-organisms and three dimensional imaging of dust in RF plasmas (2018)
  • Author(s): Akio Sanpei, Tomohito Kigami, Itaru Yoneda, Eisaku Kai, Yasuaki Hayashi, Mai Sampei
  • Organizer: 19th Workshop on Fine Particle Plasmas
  • Int'l Joint Research
• [Presentation] 浮遊状態におけるバクテリアに対するプラズマ照射とバクテリアの照射後の表面状態 (2018)
  • Author(s): 三瓶明希夫
  • Organizer: 第１回バイオメディカル研究会
  • Invited
• [Presentation] RFプラズマ中の細菌浮遊現象 (2018)
  • Author(s): 木上智仁，三瓶明希夫，米田至，林康明
  • Organizer: 第35回プラズマ・核融合学会年会
• [Presentation] RFプラズマ中の糸状微粒子の浮遊実験 (2018)
  • Author(s): 米田至, 三瓶明希夫, 木上智仁, 林康明
  • Organizer: 第35回プラズマ・核融合学会年会
• [Remarks] 京都工芸繊維大学 研究者総覧
  • URL: https://www.hyokadb.jim.kit.ac.jp/profile/ja.2f965ca8f50ea3fc94f675f4fcb5670b.html