| Project/Area Number |
18K18805
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 18:Mechanics of materials, production engineering, design engineering, and related fields
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| Research Institution | Toyohashi University of Technology |
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Principal Investigator |
SHIBATA Takayuki 豊橋技術科学大学, 工学(系)研究科(研究院), 教授 (10235575)
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| Project Period (FY) |
2018-06-29 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2019: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2018: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | 原子間力顕微鏡（AFM） / AFMナノ加工 / 酸化チタン光触媒酸化反応 / チップ増強ラマン分光法（TERS） / 細胞操作 |
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| Outline of Final Research Achievements |
With the aim of providing an innovative manufacturing technology required to support future advances in nanotechnology and biotechnology, a novel atomic force microscopy (AFM)-based nanofabrication and nanomeasurement technique has been developed. In this study, the photocatalytic nanofabrication of living cells can be performed based on highly localized photochemical oxidation with a catalytic titanium dioxide (TiO2)-functionalized AFM probe. Moreover, tip-enhanced Raman spectroscopy (TERS) imaging for analyzing the dynamic behavior of biomolecules in a living cell can be achieved with AFM probes functionalized with silver (Ag) nanoparticles.
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| Academic Significance and Societal Importance of the Research Achievements |
医療・医薬分野のさらなる発展には，生命現象の統合的理解とその制御が必要不可欠となる．このためには，ゲノム，タンパク質，糖鎖などの生体分子の構造・機能解明に加えて，生命活動の基本単位である細胞の機能を解き明かすことが，ライフ・イノベーション創出の命題となる．本研究では，生きた細胞を低侵襲で加工し，細胞内部の情報を一分子レベルで可視化する技術を確立した．細胞の機能発現過程を高度に制御・可視化する本提案技術は，生命科学の基礎研究における強力なツールとなり得る．
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