| Project/Area Number |
18K19007
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 28:Nano/micro science and related fields
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| Research Institution | Chuo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
木平 清人 国立研究開発法人宇宙航空研究開発機構, 有人宇宙技術部門, 研究開発員 (30573810)
森田 能次 中央大学, 理工学部, 助教 (40795308)
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| Project Period (FY) |
2018-06-29 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2019: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2018: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Keywords | タンパク質 / 組換えヘモグロビン / ヒト血清アルブミン / 人工酸素運搬体 / 赤血球代替物 / X線結晶構造解析 / 蛋白質 |
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| Outline of Final Research Achievements |
The realization of an artificial oxygen carrier as a red blood cell (RBC) substitute is the most important issue in next-generation medicine. A new artificial oxygen carrier “recombinant(hemoglobin-albumin)nanocluster” (rHb-rHSA3 cluster) in which recombinant human hemoglobin (rHb) is bound to recombinant human serum albumins (rHSAs) has been synthesized, and its structure and oxygen binding ability has been clarified. The variant cluster in which Leu-β28 in the heme pocket of the core rHb was replaced with Trp showed an oxygen affinity equivalent to that of RBC. We have succeeded to develop a RBC substitute that can be stably supplied and is completely independent of human blood.
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| Academic Significance and Societal Importance of the Research Achievements |
少子高齢化が進む日本では、輸血液の不足が深刻な社会問題となり始めている。また、備蓄用血液が減ると、大規模災害時に必要充分量の血液を確保できない状況に陥る危険性もある。このような背景から、血液型に関係なく、ウイルス感染の心配もなく、いつでもどこでも誰にでも使用できる人工酸素運搬体(赤血球代替物)の実現が強く望まれている。rHb-rHSA3クラスターは 輸血治療の概念を変革する新しい化合物であり、我が国の持続的成長と発展を支える基盤医療技術の一つとなり得る。
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