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Constructing artificial neuronal circuits on diamond and long-term monitoring of the spontaneous firing pattern with NV centers

Research Project

Project/Area Number 18K19026
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 29:Applied condensed matter physics and related fields
Research InstitutionWaseda University

Principal Investigator

TANII Takashi  早稲田大学, 理工学術院, 教授 (20339708)

Project Period (FY) 2018-06-29 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2020: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords神経細胞回路 / 自発発火 / NVセンター / ダイヤモンド / 細胞パターニング / NVセンタ / 神経細胞 / NVセンタ / 光検出磁気共鳴法 / 自発発火パターン
Outline of Final Research Achievements

There are two approaches to understand an extremely complex system such as the brain; namely, top-down approaches which resolve the whole system into building blocks and bottom-up approaches to construct the whole system from scratch. In this work, we aimed at (1) developing a method to detect and monitor neural spikes by combining cell-patterning techniques with NV centers in diamond for a long term and (2) understanding the spontaneous firing patterns experimentally and theoretically by constructing neuronal circuits containing only a single or a few neurons on the substrate surface.

Academic Significance and Societal Importance of the Research Achievements

ダイヤモンド中のNVセンターを電位プローブとして実神経細胞の発火を検出できる計測系の実現を試みると同時に,そのようにして基板上の構築された実神経細胞回路が提示するであろう発火パターンの理論予測の両面から研究を進めた。現時点では,NVセンターによる実神経細胞の発火計測は,当初の予測に反し,感度不足が原因で達成されていない。一方,単一または少数個の実細胞からなる神経細胞回路が発現する自発発火パターンに関する理論予測において,特にオータプスが発現する機能の観点から新たな発見があり,オータプスが神経ネットワーク全体の同期生成に深く関与していることを示唆した。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (33 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (5 results) Journal Article (6 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Open Access: 6 results) Presentation (22 results) (of which Int'l Joint Research: 11 results,  Invited: 1 results)

  • [Int'l Joint Research] CNR(イタリア)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Ulm University(ドイツ)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] CNR(イタリア)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] Ulm University(ドイツ)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] CNR(イタリア)

    • Related Report
      2018 Research-status Report
  • [Journal Article] Selective Stimulation of a Target Neuron in Micropatterned Neuronal Circuits Using a Pair of Needle Electrodes2021

    • Author(s)
      Hattori Kouhei、Kurakake Hekiru、Imai Junko、Hashimoto Takuya、Ishida Mihoko、Sato Koki、Takahashi Honoka、Oguma Soichiro、Yamamoto Hideaki、Hirano-Iwata Ayumi、Tanii Takashi
    • Journal Title

      Electrochemistry

      Volume: 89 Issue: 4 Pages: 348-354

    • DOI

      10.5796/electrochemistry.21-00032

    • NAID

      130008062336

    • ISSN
      1344-3542, 2186-2451
    • Year and Date
      2021-07-05
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Role of Noise in Spontaneous Activity of Networks of Neurons on Patterned Silicon Emulated by Noise-activated CMOS Neural Nanoelectronic Circuits2021

    • Author(s)
      Hasani Ramin、Ferrari Giorgio、Yamamoto Hideaki、Tanii Takashi、Prati Enrico
    • Journal Title

      Nano Express

      Volume: - Issue: 2 Pages: 100438-100438

    • DOI

      10.1088/2632-959x/abf2ae

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Contribution of AMPA and NMDA receptors in the spontaneous firing patterns of single neurons in autaptic culture2020

    • Author(s)
      Kouhei Hattori, Takeshi Hayakawa, Akira Nakanishi, Mihoko Ishida, Hideaki Yamamoto, Ayumi Hirano-Iwata, Takashi Tanii
    • Journal Title

      Biosystems

      Volume: 198 Pages: 104278-104278

    • DOI

      10.1016/j.biosystems.2020.104278

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Triple nitrogen-vacancy centre fabrication by C5N4Hn ion implantation2019

    • Author(s)
      M.Haruyama, S.Onoda, T.Higuchi, W.Kada, A.Chiba, Y.Hirano, T.Teraji, R.Igarashi, S.Kawai, H.Kawarada, Y.Ishii, R.Fukuda, T.Tanii, J.Isoya, T.Ohshima, O.Hanaizum
    • Journal Title

      Nature Communications

      Volume: 10 Issue: 1 Pages: 2664-2664

    • DOI

      10.1038/s41467-019-10529-x

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nitrogen-Terminated Diamond Surface for Nanoscale NMR by Shallow Nitrogen-Vacancy Centers2019

    • Author(s)
      S. Kawai, H. Yamano, T. Sonoda, K. Kato, J. Buendia, T. Kageura, R. Fukuda, T. Okada, T. Tanii, T. Higuchi, M. Haruyama, K. Yamada, S. Onoda, T. Ohshima, W. Kada, O. Hanaizumi, A. Stacey, T. Teraji, S. Kono, J. Isoya, H. Kawarada
    • Journal Title

      J. Phys. Chem. C

      Volume: 123 Issue: 6 Pages: 3594-3604

    • DOI

      10.1021/acs.jpcc.8b11274

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Impact of modular organization on dynamical richness in cortical networks2018

    • Author(s)
      H. Yamamoto, S. Moriya, K. Ide, T. Hayakawa, H. Akima, S. Sato, S. Kubota, T. Tanii, M. Niwano, S. Teller, J. Soriano, A. Hirano-Iwata
    • Journal Title

      Science Advances

      Volume: 4 Issue: 11

    • DOI

      10.1126/sciadv.aau4914

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] マイクロパターン上の神経回路における標的ニューロンへの二本針電極を用いた選択的刺激2021

    • Author(s)
      小熊奏一郎,服部晃平,鞍掛碧流,今井絢子,石田実穂子,髙橋穂乃歌,山本英明,平野愛弓,谷井孝至
    • Organizer
      2021年 第68回応用物理学会春季学術講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Extracellular stimulation of a target neuron in a micropatterned neuronal circuit using a pair of needle electrodes2020

    • Author(s)
      Kouhei Hattori, Mihoko Ishida, Honoka Takahashi, Soichiro Oguma, Takashi Tanii, Hideaki Yamamoto, Ayumi Hirano-Iwata
    • Organizer
      The 8th RIEC International Symposium on Brain Functions and Brain Computer, BFBC 2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Extracellular stimulation of a target neuron in micro-patterned neuronal circuits using a pair ofneedle electrodes2020

    • Author(s)
      Kouhei Hattori, Hekiru Kurakake, Junko Imai, Takuya Hashimoto, Mihoko Ishida, Koki Sato, Honoka Takahashi, Soichiro Oguma, Hideaki Yamamoto, Ayumi Hirano-Iwata,Takashi Tanii
    • Organizer
      PRIME2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] オータプス伝搬遅延によるSTDPシナプスの淘汰: 孤立した単一神経細胞に関する数理モデル化2020

    • Author(s)
      石田実穂子, 中西彬, 服部晃平, 山本英明, 平野愛弓, 谷井孝至
    • Organizer
      日本神経回路学会全国大会(JNNS2020)
    • Related Report
      2020 Annual Research Report
  • [Presentation] 単一NVセンター規則配列と単一細胞パターニングを用いた神経細胞回路の自発発火パターンの長期計測に向けて2019

    • Author(s)
      谷井孝至、石田実穂子、石井邑
    • Organizer
      量子生命科学会第1回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Synaptic plasticity in an isolated single autaptic neuron on a micropattern2019

    • Author(s)
      Akira Nakanishi, Kouhei Hattori, Takeshi Hayakawa, Mihoko Ishida, Hekiru Kurakake, Hideaki Yamamoto, Ayumi Hirano-Iwata, Takashi Tanii
    • Organizer
      日本神経回路学会 第29回全国大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 2本針型刺激電極の試作とマイクロパターン上神経細胞回路への刺激導入に関する実行可能性評価2019

    • Author(s)
      服部 晃平、鞍掛 碧流、今井 絢子、橋本 拓弥、佐藤 晃揮、髙橋 穂乃歌、早川 岳志、山本 英明、平野 愛弓、谷井 孝至
    • Organizer
      第80回応用物理学会秋季学術講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] Microcontact printing of scaffold proteins for engineering neuronal network functions2019

    • Author(s)
      Hideaki Yamamoto, Satoshi Moriya, Katsuya Ide, Takeshi Hayakawa, Hisanao Akima, Shigeo Sato, Shigeru Kubota, Michio Niwano, Sara Teller, Jordi Soriano, Takashi Tanii, Ayumi Hirano-Iwata
    • Organizer
      The 4th International Symposium on Creation of Life Innovation Materials for Interdisciplinary and International Researcher Development
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] A computational study on the spontaneous firing pattern of single autaptic neurons -The contribution of AMPA and NMDA current-2019

    • Author(s)
      Mihoko Ishida, Kouhei Hattori, Akira Nakanishi, Takeshi Hayakawa, Hideaki Yamamoto, Ayumi Hirano-Iwata, Takashi Tanii
    • Organizer
      The 4th International Symposium on Creation of Life Innovation Materials for Interdisciplinary and International Researcher Development
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] An experimental study on the spontaneous firing of single isolated autaptic neurons using micropatterned substrates2019

    • Author(s)
      Honoka Takahashi, Koki Sato, Mihoko Ishida, Soichiro Oguma, Takeshi Hayakawa, Kouhei Hattori, Hideaki Yamamoto, Ayumi Hirano-Iwata, Takashi Tanii
    • Organizer
      The 4th International Symposium on Creation of Life Innovation Materials for Interdisciplinary and International Researcher Development
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Self-align fabrication of nano-reservoir with NV center in diamond surface for nuclear magnetic resonance of small molecules2019

    • Author(s)
      Kazuto Kawakatsu, Yu Ishii, Yuki Hata, Yuta Saito, Kosuke Nakamura, Kirou Nagaoka, Takahiro Sonoda, Tetsuya, Tatsuishi, Tokuyuki Teraji, Shinobu Onoda, Taisei Higuchi, Keisuke Yamada, Takeshi Oshima, Takahiro Shinada, Hiroshi Kawarada, Wataru Kada, Osamu Hanaizumi, Junichi Isoya and Takashi Tanii
    • Organizer
      32nd International Microprocesses and Nanotechnology Conference
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] An experimental study on the spontaneous firing of single isolated neurons in autaptic culture using micropatterned substrates2019

    • Author(s)
      Kouhei Hattori, Koki Sato, Honoka Takahashi, Soichiro Oguma, Mihoko Ishida, Hideaki Yamamoto, Ayumi Hirano-Iwata, Takashi Tanii
    • Organizer
      the 8th RIEC International Symposium on Brain Functions and Brain Computer
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Junko Imai, Hekiru Kurakake, Takuya Hashimoto, Kouhei Hattori, Koki Sato, Honoka Takahashi, Soichiro Oguma, Mihoko Ishida, Hideaki Yamamoto, Ayumi Hirano-Iwata, Takashi Tanii2019

    • Author(s)
      Junko Imai, Hekiru Kurakake, Takuya Hashimoto, Kouhei Hattori, Koki Sato, Honoka Takahashi, Soichiro Oguma, Mihoko Ishida, Hideaki Yamamoto, Ayumi Hirano-Iwata, Takashi Tanii
    • Organizer
      the 8th RIEC International Symposium on Brain Functions and Brain Computer
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 高分解能ナノ NMR に向けたダイヤモンドへの NV センターと微小容器の一括形成2019

    • Author(s)
      齋藤悠太、石井邑、川勝一斗、永岡希朗、畑雄貴、中村洸介、榎田尊昭、徐海州、薗田隆弘、立石哲也、金久京太郎、寺地徳之、小野田忍、樋口泰成、山田圭介、大島武、品田高宏、川原田洋、加田渉、花泉修、磯谷順一、谷井孝至
    • Organizer
      第67回応用物理学会春季学術講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] マイクロパターン上の神経細胞への2針電極による刺激とその応答計測2019

    • Author(s)
      鞍掛 碧流, 服部 晃平, 今井 絢子, 橋本 拓弥, 佐藤 晃揮, 髙橋 穂乃歌, 小熊 奏一郎, 石田 実穂子, 山本 英明, 平野 愛弓, 谷井 孝至
    • Organizer
      第67回応用物理学会春季学術講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] イオン注入による単一不純物欠陥の規則的配列形成をその応用2019

    • Author(s)
      谷井孝至、品田高宏、寺地徳之、小野田忍、大島武、McGuuinness Liam, Jelezko Fedor, Liu Yan, Wu E, 加田渉、花泉修、川原田洋、磯谷順一
    • Organizer
      第66回応用物理学会春季学術講演会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Surface modification with visible-light-responsible TiO2 thin film2019

    • Author(s)
      Atsushi Kurotobi, Sho Kono, Takayuki Ichikawa, Hideaki Yamamoto, Ayumi Hirano-Iwata and Tanii Takashi
    • Organizer
      The 7th RIEC International Symposium on Brain Functions and Brain Computer
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] An experimental study on spontaneous firing of a single neuron on micropatterned substrates2019

    • Author(s)
      Koki Sato, Takahiro Nakane, Honoka Takahashi, Kouhei Hattori, Hideaki Yamamoto, Ayumi Hirano-Iwata and Takashi Tanii
    • Organizer
      The 7th RIEC International Symposium on Brain Functions and Brain Computer
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Computational modeling of spontaneous firing patterns generated by single autaptic neurons2019

    • Author(s)
      Kouhei. Hattori, Takeshi Hayakawa, Akira Nakanishi, Mihoko Ishida, Hideaki Yamamoto, Ayumi Hirano-Iwata, Takashi Tanii
    • Organizer
      The 7th RIEC International Symposium on Brain Functions and Brain Computer
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] ダイヤモンド中単一NVセンターのパルス光磁気共鳴測 定のためのローエンドFPGAへのフォトンカウンタの実装2019

    • Author(s)
      永岡 希朗, 畑 雄貴, 川勝 一斗, 石井 邑, 福田 諒 介, 寺地 徳之, 小野田 忍, 大島 武, 品田 高宏 , 川 原田 洋, 磯谷 順一, 谷井 孝至
    • Organizer
      第66回応用物理学会春季学術講演会
    • Related Report
      2018 Research-status Report
  • [Presentation] 高被覆率窒素終端(111)ダイヤモンドの作製2019

    • Author(s)
      第66回応用物理学会春季学術講演会
    • Organizer
      第66回応用物理学会春季学術講演会
    • Related Report
      2018 Research-status Report
  • [Presentation] C5N4Hnイオン注入による双極子結合したNVセンターの 形成2019

    • Author(s)
      春山 盛善, 小野田 忍, 樋口 泰成, 加田 渉, 千 葉 敦也, 平野 貴美, 寺地 徳之, 五十嵐 龍治, 河合 空, 川原田 洋, 石井 邑, 福田 諒介, 谷井 孝至, 磯谷 順一, 大島 武, 花泉 修
    • Organizer
      第66回応用物理学会春季学術講演会
    • Related Report
      2018 Research-status Report

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Published: 2018-07-25   Modified: 2022-01-27  

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