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Ion concentration imaging system with electron beam excitation for observation of a single ion channel

Research Project

Project/Area Number 18K19032
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 30:Applied physics and engineering and related fields
Research InstitutionShizuoka University

Principal Investigator

KAWATA Yoshimasa  静岡大学, 電子工学研究所, 教授 (70221900)

Co-Investigator(Kenkyū-buntansha) 居波 渉  静岡大学, 電子工学研究所, 教授 (30542815)
Project Period (FY) 2018-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Keywords光学顕微鏡 / 電子顕微鏡 / イオンチャネル / イオンセンサー / イオン感応膜 / 半導体 / 超解像顕微鏡
Outline of Final Research Achievements

In this research, we have developed ion-sensitive imaging system with electron beam excitation and we try to apply the developed system for the observation of a single ion channel. The unique advantage of the developed system is possible to observe ion concentration distribution with higher spatial resolution than that of the conventional ion imaging system. The spot size of focused electron beam in an ion sensitive substrate was simulated with Monte-Carlo method and the resolution was evaluated. The layer structure of ion-sensitive substrate was optimized with the simulation results. The function of ion sensor was verified by changing a bias voltage and evaluated in measurement of ion concentration.

Academic Significance and Societal Importance of the Research Achievements

本提案は、イオン感応膜上の電荷分布の検出に集束電子線を用いて、イオンセンシングシステムの空間分解能を飛躍的に向上させる点に新規性があり、世界的に見ても全く例のない、研究代表者らのこれまでの研究実績に基づく独創性の高い研究である。本システムを実現することにより、イオンセンサーの空間分解能を飛躍的に向上させることができ、ナノ領域のイオン移動のダイナミクスを明らかにすることができる。本システムを細胞試料の観察に応用すればイオンチャンネルの機能解明が可能となる。イオン感応膜上と電子線との相互作用の理論構築、ナノ領域におけるイオン挙動の解明、などさまざま分野への波及効果が期待できる。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (45 results)

All 2020 2019 2018

All Journal Article (12 results) (of which Peer Reviewed: 12 results,  Open Access: 9 results) Presentation (33 results) (of which Int'l Joint Research: 10 results,  Invited: 19 results)

  • [Journal Article] Second harmonic conversions of surface-plasmon-polariton?enhanced optical fields in nonlinear optics polymer/Ag/glass structures2020

    • Author(s)
      Sugita Atsushi、Kaname Suto、Mochizuki Kannta、Kikuchi Kohei、Ono Atsushi、Inami Wataru、Kawata Yoshimasa
    • Journal Title

      Physical Review B

      Volume: 101 Issue: 4

    • DOI

      10.1103/physrevb.101.045303

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ultrashort laser based two-photon phase-resolved fluorescence lifetime measurement method2020

    • Author(s)
      Nor Hafizah Binti Mohamed Halip, Yuto Yoshimura, Wataru Inami, Yoshimasa Kawata
    • Journal Title

      Methods and Applications in Fluorescence

      Volume: 8 Issue: 2 Pages: 025003-025003

    • DOI

      10.1088/2050-6120/ab71c2

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Highly Luminescent YAIO3:Gd3+ thin films as a nanoscale optical source for super-resolution bioimating2019

    • Author(s)
      Mykyta Kolchiba, Wataru Inami, Yoshimasa Kawata
    • Journal Title

      Optical Material Express

      Volume: 9 Pages: 1803-1814

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cell stimulation by focused electron beam of atmospheric SEM2019

    • Author(s)
      Wataru Inami, Daiki Horiba, Yoshimasa Kawata
    • Journal Title

      Ultramicroscopy

      Volume: 206 Pages: 112823-112823

    • DOI

      10.1016/j.ultramic.2019.112823

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Visualization of ultraviolet absorption distribution beyond the diffraction limit of light by electron‐beam excitation‐assisted optical microscope2019

    • Author(s)
      Wataru Inami, Masahiro Fukuta, Yoshimasa Kawata, Susumu Terakawa
    • Journal Title

      Journal of Microscopy

      Volume: 276 Issue: 1 Pages: 46-50

    • DOI

      10.1111/jmi.12835

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 深紫外プラズモニクスと高感度蛍光バイオイメージング2019

    • Author(s)
      川田善正
    • Journal Title

      光アライアンス

      Volume: 6 Pages: 41-45

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 深紫外光励起表面プラズモンと高感度蛍光バイオイメージング2019

    • Author(s)
      川田善正、小野篤史、居波 渉
    • Journal Title

      化学工業「プラズモニクスの新展開」

      Volume: 70 Pages: 15-21

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Numerical simulations of partially coherent illumination for multi-scattering phase objects via a beam propagation method2019

    • Author(s)
      Yoshimasa Suzuki, Satoshi Watanabe, Mayumi Odaira, Toshiro Okamura, and Yoshimasa Kawata
    • Journal Title

      Applied Optics

      Volume: 58 Issue: 4 Pages: 954-962

    • DOI

      10.1364/ao.58.000954

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Highly Luminescent YAIO3:Gd3+ Thin Films as a Nanoscale Optical Source for Super-Resolution Bioimating2019

    • Author(s)
      Mykyta Kolchiba, Wataru Inami, Yoshimasa Kawata
    • Journal Title

      Optical Material Express

      Volume: 9 Issue: 4 Pages: 1803-1814

    • DOI

      10.1364/ome.9.001803

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] A Time-Resolved Four-Tap Lock-In Pixel CMOS Image Sensor for Real-Time Fluorescence Lifetime Imaging Microscopy2018

    • Author(s)
      Seo Min-Woong、Shirakawa Yuya、Kawata Yoshimasa、Kagawa Keiichiro、Yasutomi Keita、Kawahito Shoji
    • Journal Title

      IEEE Journal of Solid-State Circuits

      Volume: 53 Issue: 8 Pages: 2319-2330

    • DOI

      10.1109/jssc.2018.2827918

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Photothermally Active Upconversion Core-Shell NaGdF4:Yb:Tm@Cu Nanostructures: Synthesis and Theranostic Properties2018

    • Author(s)
      Ibrahim Khaleelullah Mohamed Mathar Sahib、Tanaka Asahi、Dheivasigamani Thangaraju、Sugimoto Kyohei、Shimura Yosuke、Inami Wataru、Kawata Yoshimasa、Hayakawa Yasuhiro
    • Journal Title

      Particle & Particle Systems Characterization

      Volume: 35 Issue: 11 Pages: 1800227-1800227

    • DOI

      10.1002/ppsc.201800227

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Fluorescence Lifetime Measurement Excited with Ultraviolet Surface Plasmon Resonance2018

    • Author(s)
      Hanulia Taras、Inami Wataru、Ono Atsushi、Kawata Yoshimasa
    • Journal Title

      Optics Communications

      Volume: 427 Pages: 266-270

    • DOI

      10.1016/j.optcom.2018.06.069

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] High resolution imaging of ion-distribution with electron beam excitation2019

    • Author(s)
      Yoshimasa Kawata
    • Organizer
      Global Nanophotonics 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] High Resolution Bio-Imaging with Electron Beam Excitation assisted (EXA) optical microscopy2019

    • Author(s)
      Yoshimasa Kawata
    • Organizer
      12th International Symposium on Atomic Level Characterizations for New Materials and Devices '19 (ALC'19)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] High Resolution Bio-Imaging of Non-Stained Cells with Electron Beam Excitation2019

    • Author(s)
      Yoshimasa Kawata
    • Organizer
      Joint Student Scientific Seminar Between Faculty of Engineering & Physics of Amur State University, Russia and Faculty of Engieering of National University Corporation Shizuoka University, Japan
    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] High Resolution Bio-imaging of Non-Stained Cells with Electron Beam Excitation2019

    • Author(s)
      Yoshimasa Kawata
    • Organizer
      5th International Conference on Nanoscience and Nanotechnology (ICONN2019)
    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Fluorescence Enhancement by Electron Beam Excitation LSPR2019

    • Author(s)
      Tatsuya Yamamoto, Atsushi Ono, Wataru Inami, Yoshimasa Kawata
    • Organizer
      Optics + Photonics 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Highly Luminescent YAlO3:Gd3+ Thin Films as a Nanoscale Optical Source for Super-resolution Microscopy2019

    • Author(s)
      Mykyta Kolchiba, Wataru Inami, Yoshimasa Kawata
    • Organizer
      The 4th International Symposium toward the Future of Advanced Researches in Shizuoka University
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Two-photon Phase-resolved Fluorescence Lifetime Measurement Method Using Ultrashort Pulse Laser2019

    • Author(s)
      Nor Hafizah Binti Mohamed Halip, Yuto Yoshimura, Wataru Inami, Yoshimasa Kawata
    • Organizer
      The 4th International Symposium toward the Future of Advanced Researches in Shizuoka University
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 静岡大学と浜松医科大学の統合再編とバイオ観察のための超解像顕微鏡の開発2019

    • Author(s)
      川田 善正
    • Organizer
      2019年度浜松商工会湘南支部総会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Two-photon Phase-resolved Fluorescence-lifetime-measurement Method Involving Use of Ultrashort-pulsed Laser2019

    • Author(s)
      Nor Hafizah Binti Mohamed Halip, Wataru Inami, and Yoshimasa Kawata
    • Organizer
      2019 年日本分光学会年次講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 静岡大学工学部の紹介と超解像顕微鏡の進展2019

    • Author(s)
      川田善正
    • Organizer
      室蘭工業大学訪問シンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 電子線励起による超解像光学顕微鏡2019

    • Author(s)
      川田善正
    • Organizer
      特定非営利活動法人 日本フォトニクス協議会, JPC アカデミック・パートナーシップ・ゼミナール (APS) 第5回 「静岡大学 -次世代イメージングを目指したひかり技術-」
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 電子線照射による人工細胞膜のダメージ評価2019

    • Author(s)
      山本秀介、居波渉、手老龍吾、川田善正
    • Organizer
      本光学会年次学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 電子線励起高空間分解能イオンイメージングのための基板構造の最適化2019

    • Author(s)
      新井清久, 居波渉, 川田善正
    • Organizer
      2019年第80回応用物理学会秋季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 高空間分解能イオンイメージングシステムのセンサー基板の評価2019

    • Author(s)
      柴野暁, 川田善正, 居波渉
    • Organizer
      2019年第66回応用物理学会春季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ドルマン型金ナノ粒子複合系の超高速時間分解吸収分光2019

    • Author(s)
      浜田勝平, 松井大海, 小野篤史, 居波渉, 川田善正, 吉澤雅幸, 杉田篤史
    • Organizer
      2019年第66回応用物理学会春季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 2光子過程を用いた深紫外励起顕微鏡に関する研究2019

    • Author(s)
      山本雄大, 川田善正, 居波 渉
    • Organizer
      情報フォトニクス研究会第13回関東学生研究論文講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 細胞膜観察のための高分解能イオンイメージングシステムの開発2019

    • Author(s)
      冨田光, 川田善正, 居波 渉
    • Organizer
      情報フォトニクス研究会第13回関東学生研究論文講演会講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 電子線の侵入長の違いを用いた走査型電子顕微鏡による3次元構造観察2019

    • Author(s)
      中村竣亮, 川田善正, 居波 渉
    • Organizer
      情報フォトニクス研究会第13回関東学生研究論文講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 共焦点顕微鏡を用いた精密濾過の3次元動態観察2019

    • Author(s)
      小出瑛大, 川田善正, 居波 渉
    • Organizer
      情報フォトニクス研究会第13回関東学生研究論文講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 水溶液への電子線照射による金属微細構造の作製2019

    • Author(s)
      三輪直也, 川田善正, 居波 渉
    • Organizer
      情報フォトニクス研究会第13回関東学生研究論文講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 電子線励起局在プラズモンを用いた蛍光増強の研究2019

    • Author(s)
      山本 達也, 小野 篤史, 居波 渉, 川田 善正
    • Organizer
      一般社団法人レーザー学会学術講演会 第39回年次大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 電子線励起アシスト超解像顕微鏡2019

    • Author(s)
      川田善正
    • Organizer
      第45回冬期講習会「光と生命科学」
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 光を用いた超解像バイオイメージング2019

    • Author(s)
      川田善正
    • Organizer
      東三河・浜松地区高大連携協議会フォーラム
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 電子線励起蛍光顕微鏡2019

    • Author(s)
      川田善正、居波 渉
    • Organizer
      マイクロビームアナリシス 第141委員会 第175回 研究会資料 日本学術振興会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 電子線励起による超解像光学顕微鏡2019

    • Author(s)
      川田善正
    • Organizer
      特定非営利活動法人 日本フォトニクス協議会, JPC アカデミック・パートナーシップ・ゼミナール (APS) 第5回 「静岡大学 -次世代イ メージングを目指したひかり技術-」
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Electron Beam Addressable Potentiometric Sensor for Ion Distribution Imaging with High Resolution2018

    • Author(s)
      Wataru Inami, Kiyohisa Nii, Satoru Shibano, Yoshimasa Kawata
    • Organizer
      The 4th Biomedical Imaging and Sensing (Bisc2018)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] High Spatial Resolution Ion Imaging with Potentiometric Sensor Using an Electron Beam2018

    • Author(s)
      Wataru Inami, Kiyohisa Nii, Satoru Shibano, Hikaru Tomita, Yoshimasa Kawata, Yuji Miyahara
    • Organizer
      The 4th International Conference on Nano Electronics Research Education 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Optics and Photonics2018

    • Author(s)
      Yoshimasa Kawata
    • Organizer
      Intensive Course at National Taiwan University of Science and Technology
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] バイオイメージングのための超解像顕微鏡の開発2018

    • Author(s)
      川田善正
    • Organizer
      浜松工業会 東京支部総
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 結像の評価と近接場光学2018

    • Author(s)
      川田善正
    • Organizer
      2018光応用技術研修会テキスト
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 電子線励起による超解像光学顕微鏡(EXA)の開発2018

    • Author(s)
      川田善正、居波 渉
    • Organizer
      第23回 福井セミナー
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 深紫外プラズモニクスと高感度バイオイメージング2018

    • Author(s)
      川田善正
    • Organizer
      光とレーザーの科学技術フェア2018」レーザーセミナー講演予稿集
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 紫外領域における表面プラズモン共鳴の現象研究とその応用2018

    • Author(s)
      川田善正
    • Organizer
      平成30年度多元技術融合光プロセス研究会第4回研究交流会
    • Related Report
      2018 Research-status Report
    • Invited

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Published: 2018-07-25   Modified: 2021-02-19  

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