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Challenge to gold biomineral processing incorporating enzyme decomposition of carbonaceous matters

Research Project

Project/Area Number 18K19045
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 31:Nuclear engineering, earth resources engineering, energy engineering, and related fields
Research InstitutionKyushu University

Principal Investigator

Sasaki Keiko  九州大学, 工学研究院, 教授 (30311525)

Project Period (FY) 2018-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords趙難処理金鉱石 / 炭素質物質 / シアン化処理 / QEMSCAN / リグニン分解性酵素 / 粘土鉱物 / 白色腐朽菌 / 鉄酸化菌 / 炭素質頁岩 / 金鉱石 / バイオミネラルプロセッシング / 超難処理金鉱石 / リグニンペルオキシダーゼ / マンガンペルオキシダーゼ / 硫化鉱物 / 金回収率 / 超難処理型金鉱石 / リグニン分解酵素
Outline of Final Research Achievements

Double refractory gold ore (DRGO) includes higher gold contents but lower gold recovery than other gold ore. The recovery loss is caused by adsorption of Au(CN)2- on carbonaceous matter in DRGO. From both aspects of efficiency and environmental protection, satisfying solution has not yet been established. In the present work, without using high temperature and high pressure, a novel gold recovery was aimed to establish using biotechnology involving enzyme reaction to degrade carbonaceous matter. Decomposition of sulfide minerals by iron-oxidizing microorganisms followed by degradation of carbonaceous matter by secreted enzyme from a white rot-fungus improved a gold recovery from DRGO by cyanidation from 24% to 92%. Lignin degrading enzyme is responsible for degradation of carbonaceous matter in DRGO.

Academic Significance and Societal Importance of the Research Achievements

本研究では、固体残差のQEMSCAN分析結果から主要反応を裏付ける傍証を初めて示した。炭素質金鉱石の金および炭素分は微量成分であり、それぞれのバイオ反応の前後での変化を直接追うことは極めて困難である。しかし、リグニン分解性酵素による炭素質物質の分解による生成物中間体は、鉱石の主成分である粘土鉱物と大きな複合体を形成することを、QEMSCANにより明らかにした。この複合体をアルカリ処理すると、金回収率がさらに16%向上した。腐植様物質と粘土鉱物の複合体の生成機構、この複合体と金の微粒子との相互作用を明らかにし、直接観察が困難な対象成分に対して、逐次的バイオプロ セスの本質的なポイントを解明した。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (22 results)

All 2019 2018 Other

All Int'l Joint Research (6 results) Journal Article (4 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 4 results) Presentation (11 results) (of which Int'l Joint Research: 8 results,  Invited: 6 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] ケープタウン大学(南アフリカ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] ペンシルバニア州立大学(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] ガーナ鉱山工科大学(ガーナ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] カーティン大学/CSIRO(オーストラリア)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] ケープタウン大学(南アフリカ)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] ペンシルバニア州立大学(米国)

    • Related Report
      2018 Research-status Report
  • [Journal Article] Biomineral Processing of Graphitic Refractory Gold Ores Using Enzymatic Reactions2019

    • Author(s)
      笹木 圭子、コジョ トワム コナドゥ
    • Journal Title

      THE MICROMERITICS

      Volume: 63 Issue: 0 Pages: 29-36

    • DOI

      10.24611/micromeritics.2020008

    • NAID

      130007761311

    • ISSN
      0429-9051, 2432-2075
    • Year and Date
      2019-12-15
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sequential pretreatment of double refractory gold ore (DRGO) with a thermophilic iron oxidizing archeaon and fungal crude enzymes2019

    • Author(s)
      Konadu Kojo T.、Huddy Robert J.、Harrison Susan T.L.、Osseo-Asare Kwadwo、Sasaki Keiko
    • Journal Title

      Minerals Engineering

      Volume: 138 Pages: 86-94

    • DOI

      10.1016/j.mineng.2019.04.043

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Transformation of the carbonaceous matter in double refractory gold ore by crude lignin peroxidase released from the white-rot fungus2019

    • Author(s)
      Konadu Kojo T.、Harrison Susan T.L.、Osseo-Asare Kwadwo、Sasaki Keiko
    • Journal Title

      International Biodeterioration & Biodegradation

      Volume: 143 Pages: 104735-104735

    • DOI

      10.1016/j.ibiod.2019.104735

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effect of washing on the sequential bio-oxidation of double refractory gold ore by white-rot fungus Phanerochaete chrysosporium and thermophilic archaeron Acidianus brierleyi2018

    • Author(s)
      Kojo T. Konadu, Niko D. Pahlevi, Susan H. L. Harrison, Kwadwo Osseo-Asare, Keiko Sasaki
    • Journal Title

      Proc. International Symposium on Earth Science and Technology

      Volume: 1 Pages: 326-329

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Fungally formed Mn oxides and their application to bio-templated Li+ ion sieve2019

    • Author(s)
      Keiko Sasaki
    • Organizer
      23th International Biohydrometallutgy Symposium 2019 (Fukuoka)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Bio-mineral processing of double refractory gold ore in sequential treatment using a thermophilic archaeon followed by fungal spent medium2019

    • Author(s)
      Kojo T. Konadu, Susan T. L. Harrison, Kwadwo Osseo-Asare, Keiko Sasaki
    • Organizer
      23th International Biohydrometallutgy Symposium 2019 (Fukuoka)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effect of carbonaceous matter on bioleaching of Cu from carbonaceous chalcopyrite ore2019

    • Author(s)
      Kojo T. Konadu, Ryotaro Sakai, Diego M. M. Flores, Susan T. L. Harrison, Kwadwo Osseo- Asare, Keiko Sasaki
    • Organizer
      23th International Biohydrometallutgy Symposium 2019 (Fukuoka)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Determination of lignin-modifying enzymes by capillary electrophoresis using in-capillary reactions2019

    • Author(s)
      Takashi Kaneta, Airi Harada, Sumire Kudo, Hiroe Kubota, Keiko Sasaki
    • Organizer
      23th International Biohydrometallutgy Symposium 2019 (Fukuoka)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Challenges to gold recovery from carbonaceous gold ores using biotechnology2019

    • Author(s)
      Kojo Konadu, Keiko Sasaki
    • Organizer
      African MRS 2019 (Arusha)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Challenges to gold recovery from carbonaceous gold ores using biotechnology2019

    • Author(s)
      Kojo Twum Konadu, Keiko Sasaki
    • Organizer
      1st International Conference of Asia Pacific Mining and Metallurgy
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] バイオテクノロジーによる難処理金鉱石からの金回収率の向上2019

    • Author(s)
      笹木圭子
    • Organizer
      日本技術士会 地域産学官と技術士との合同セミナー
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 酵素反応をとりいれたグラファイト質難処理金鉱石のミネラルプロセッシング2019

    • Author(s)
      笹木圭子
    • Organizer
      粉体工学会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 超難処理金鉱石のバイオハイドロメタラジー2019

    • Author(s)
      笹木圭子
    • Organizer
      東北大学多元物質研究所研究発表会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Biohydrometallurgy for sustainable development goals2019

    • Author(s)
      Keiko Sasaki
    • Organizer
      Workshop in National Taipei University of Technology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Effect of washing on the sequential bio-oxidation of double refractory gold ore by white-rot fungus Phanerochaete chrysosporium and thermophilic archaeron Acidianus brierleyi2018

    • Author(s)
      Kojo T. Konadu, Niko D. Pahlevi, Susan H. L. Harrison, Kwadwo Osseo-Asare, Keiko Sasaki
    • Organizer
      International Symposium on Earth Science and Technology (CINEST 2018)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Patent(Industrial Property Rights)] 金鉱石の前処理方法および金回収方法2019

    • Inventor(s)
      笹木圭子、コナドゥ コジョ トワム他5名
    • Industrial Property Rights Holder
      笹木圭子、コナドゥ コジョ トワム他5名
    • Industrial Property Rights Type
      特許
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report

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Published: 2018-07-25   Modified: 2021-02-19  

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