| Project/Area Number |
18K19090
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 34:Inorganic/coordination chemistry, analytical chemistry, and related fields
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| Research Institution | Gunma University |
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Principal Investigator |
Sato Kiichi 群馬大学, 大学院理工学府, 教授 (50321906)
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| Project Period (FY) |
2018-06-29 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Keywords | マイクロ流体デバイス / 臓器モデル / 腎臓 / 腎モデル / 糸球体 / バイオアッセイ |
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| Outline of Final Research Achievements |
The glomerulus of the kidney plays an important role in filtering primary urine from the blood, and this filtration barrier is composed of podocytes, basement membrane, and glomerular endothelial cells. In this study, we tried to construct a glomerular model on a microfluidic device. We established the conditions for culturing normal human podocyte cell lines and normal human glomerular endothelial cell lines in a device fabricated using polydimethylsiloxane (PDMS) and membrane filters. Furthermore, we realized the construction of a disease model by adding disease factors and compared it with the normal model by cell observation and permeability test.
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| Academic Significance and Societal Importance of the Research Achievements |
動物実験削減が推奨される中、臓器モデルやその疾病モデルは病態解析や創薬のための強力な分析ツールとして期待され、様々な臓器や組織のモデルの開発が注目されている。開発したシステムが実用化できれば腎疾患に関する治療・創薬研究の発展に大いに貢献できるものと期待され、さらには血管や消化管など、生体内のさまざまな管腔における物質透過性やその疾病に関するモデルへの幅広い応用が期待される。
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