Development of an analytical method for optical control of membrane proteins using the optical properties of lanthanide nanoparticles
| Project/Area Number |
18K19092
|
|---|
| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Medium-sized Section 34:Inorganic/coordination chemistry, analytical chemistry, and related fields
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
Ozawa Takeaki 東京大学, 大学院理学系研究科(理学部), 教授 (40302806)
|
|---|
| Project Period (FY) |
2018-06-29 – 2022-03-31
|
| Project Status |
Completed (Fiscal Year 2021)
|
| Budget Amount *help |
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
|
|---|
| Keywords | アップコンバージョン / 光操作 / ナノ粒子 / 膜タンパク質 / オプトジェネティクス / レセプター / 近赤外 |
|---|
| Outline of Final Research Achievements |
A novel analytical method was developed to manipulate the activity of specific membrane receptors in living cells using near-infrared light. Upconversion particles (LNPs) that absorb near-infrared light and emit blue light were successfully targeted and retained in mouse liver. We also developed a new cell membrane receptor that can be manipulated by blue light, and expressed it in mouse liver using an adenovirus. When the abdomen of mice was irradiated with near-infrared light, LNPs that absorbed near-infrared light emitted blue light in the liver and increased the membrane receptor activity. Furthermore, we confirmed an increase in phosphorylation activity of intracellular signals.
|
| Academic Significance and Societal Importance of the Research Achievements |
組織透過性の高い近赤外光をナノ粒子で青色光に組織内で変換することで，膜タンパク質の光操作が実現可能となることを実証できた学術的意義は大きい．ナノ粒子を肝臓に滞留させる新たな技術は高い汎用性が期待できる．既存の光操作ツール一般に応用展開が可能であり，生物学的な基礎研究を始め，創薬や医学分野など様々な波及効果が期待できる技術である．
|
Report
(5 results)
Research Products
(35 results)
