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Development of an analytical method for optical control of membrane proteins using the optical properties of lanthanide nanoparticles

Research Project

Project/Area Number 18K19092
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 34:Inorganic/coordination chemistry, analytical chemistry, and related fields
Research InstitutionThe University of Tokyo

Principal Investigator

Ozawa Takeaki  東京大学, 大学院理学系研究科(理学部), 教授 (40302806)

Project Period (FY) 2018-06-29 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywordsアップコンバージョン / 光操作 / ナノ粒子 / 膜タンパク質 / オプトジェネティクス / レセプター / 近赤外
Outline of Final Research Achievements

A novel analytical method was developed to manipulate the activity of specific membrane receptors in living cells using near-infrared light. Upconversion particles (LNPs) that absorb near-infrared light and emit blue light were successfully targeted and retained in mouse liver. We also developed a new cell membrane receptor that can be manipulated by blue light, and expressed it in mouse liver using an adenovirus. When the abdomen of mice was irradiated with near-infrared light, LNPs that absorbed near-infrared light emitted blue light in the liver and increased the membrane receptor activity. Furthermore, we confirmed an increase in phosphorylation activity of intracellular signals.

Academic Significance and Societal Importance of the Research Achievements

組織透過性の高い近赤外光をナノ粒子で青色光に組織内で変換することで,膜タンパク質の光操作が実現可能となることを実証できた学術的意義は大きい.ナノ粒子を肝臓に滞留させる新たな技術は高い汎用性が期待できる.既存の光操作ツール一般に応用展開が可能であり,生物学的な基礎研究を始め,創薬や医学分野など様々な波及効果が期待できる技術である.

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (35 results)

All 2022 2021 2020 2019 2018 Other

All Int'l Joint Research (4 results) Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 3 results) Presentation (24 results) (of which Int'l Joint Research: 12 results,  Invited: 23 results)

  • [Int'l Joint Research] NTU(シンガポール)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] NTU(シンガポール)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] NTU(シンガポール)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] 南洋理工大学(シンガポール)

    • Related Report
      2018 Research-status Report
  • [Journal Article] Castanospermine suppresses CD44 ectodomain cleavage as revealed by transmembrane bioluminescent sensors2022

    • Author(s)
      Noda Natsumi、Ozawa Takeaki
    • Journal Title

      Journal of Cell Science

      Volume: 135 Issue: 6 Pages: 259314-259314

    • DOI

      10.1242/jcs.259314

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Optogenetic Control of Phosphatidylinositol (3,4,5)‐Triphosphate Production by <scp>Light‐Sensitive</scp> Cryptochrome Proteins on the Plasma Membrane2021

    • Author(s)
      Yang Lingzhi、Ozawa Takeaki、Dong Haifeng、Zhang Xueji
    • Journal Title

      Chinese Journal of Chemistry

      Volume: 39 Issue: 5 Pages: 1240-1246

    • DOI

      10.1002/cjoc.202000648

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Functional Modulation of Receptor Proteins on Cellular Interface with Optogenetic System2021

    • Author(s)
      Endo Mizuki、Ozawa Takeaki
    • Journal Title

      Advances in Experimental Medicine and Biology

      Volume: 1293 Pages: 247-263

    • DOI

      10.1007/978-981-15-8763-4_15

    • ISBN
      9789811587627, 9789811587634
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Light-mediated Control of Gene Expression in Mammalian Cells.2020

    • Author(s)
      Yamada M, Nagasaki C. S, Ozawa T, Imayoshi I.
    • Journal Title

      Neuroscience Research

      Volume: 152 Pages: 66-77

    • DOI

      10.1016/j.neures.2019.12.018

    • NAID

      120006888020

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Photocleavable Cadherin Inhibits Cell-to-Cell Mechanotransduction by Light2019

    • Author(s)
      Endo Mizuki、Iwawaki Takumi、Yoshimura Hideaki、Ozawa Takeaki
    • Journal Title

      ACS Chemical Biology

      Volume: 14 Pages: 2206-2214

    • DOI

      10.1021/acschembio.9b00460

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Optical control of G protein-coupled receptor activities in living cells2019

    • Author(s)
      H. Yoshimura and T. Ozawa
    • Journal Title

      Progress in Photon Science

      Volume: 119 Pages: 129-138

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Unique Roles of β-Arrestin in GPCR Trafficking Revealed by Photoinducible Dimerizers2018

    • Author(s)
      Takenouchi Osamu、Yoshimura Hideaki、Ozawa Takeaki
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 1-11

    • DOI

      10.1038/s41598-017-19130-y

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 生体分子を光で観る・操作する技術の新展開2021

    • Author(s)
      小澤岳昌
    • Organizer
      光イメージング若手の会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Protein-based Luminescent Sensors and Optical Switches for Cell Analysis2021

    • Author(s)
      T. Ozawa
    • Organizer
      PITTCON2021
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 細胞膜レセプター機能解析のための新たなイメージングと光操作法2020

    • Author(s)
      小澤岳昌
    • Organizer
      日本分析化学会第69年会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] 2.光で操作し観察する新たな細胞膜リセプターの機能解析法2019

    • Author(s)
      小澤岳昌
    • Organizer
      イスマートセルイノベーション研究センター講演会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Optical Sensors and Switches for Live Cell Analysis: Opto-bioanalysis2019

    • Author(s)
      T. Ozawa
    • Organizer
      3rd Asian Conference on Chemosensors & Imaging Probes (AsianChIP-2019)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Imaging and controlling membrane receptor activities in living cells2019

    • Author(s)
      T. Ozawa
    • Organizer
      Hwasun Optical & Molecular Imaging Workshop and Symposium (HOWS)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Optical Switches of Membrane Receptor Activities Using CRY22019

    • Author(s)
      T. Ozawa
    • Organizer
      生物物理学会第57回年会(宮崎)
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Imaging and controlling membrane receptor activities in living cells2019

    • Author(s)
      T. Ozawa
    • Organizer
      The 6th International Symposium on Bioimaging (Tokyo)
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 生細胞解析を目指した改変型発光タンパク質の開発2019

    • Author(s)
      小澤岳昌
    • Organizer
      生化学会大会(横浜)
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 光で操作し観察する新たな細胞機能解析技術2019

    • Author(s)
      小澤岳昌
    • Organizer
      イメージングブートキャンプ2019(札幌)
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 細胞膜リセプターの機能を観る・操作する新たな技術2019

    • Author(s)
      小澤岳昌
    • Organizer
      生命化学研究会ポストコンファレンス(女満別)
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 生命の神秘を光で探る―オプトバイオアナリシス―2019

    • Author(s)
      Takeaki Ozawa
    • Organizer
      東京大学理学部公開講演会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Protein-based luminescent sensors for single cell analysis2018

    • Author(s)
      Takeaki Ozawa
    • Organizer
      JSPS-NTS Bilateral Symposium on Functional Materials Chemistry
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 光で生体分子を操作し観察する新たな細胞解析技術2018

    • Author(s)
      Takeaki Ozawa
    • Organizer
      日本分光学会年次講演会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Opto-bioanalysis: Imaging and controlling GPCR activities in living cells2018

    • Author(s)
      Takeaki Ozawa
    • Organizer
      IUBMB
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 生細胞内GPCR動態の可視化と制御2018

    • Author(s)
      Takeaki Ozawa
    • Organizer
      生理研研究会
    • Related Report
      2018 Research-status Report
  • [Presentation] Imaging and Controlling GPCR activities in live cells2018

    • Author(s)
      Takeaki Ozawa
    • Organizer
      The 5th Asian Chemical Biology Conference
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 光でタンパク質を操作し観察する細胞解析技術―オプトバイオアナリシス―2018

    • Author(s)
      Takeaki Ozawa
    • Organizer
      日本分析化学会第67年会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Imaging and Controlling Protein Activities in Living Cells: Opto-bioanalysis2018

    • Author(s)
      Takeaki Ozawa
    • Organizer
      6th UT-UDS Joint Symposium on “Symmetry and Asymmetry in Science”
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Imaging and Manipulating Intracellular signals in Single Live Cells with External Light.2018

    • Author(s)
      Takeaki Ozawa
    • Organizer
      the 10th International Forum on Post-Genomic Technology (IFPT’10) and the 11th International Workshop on Approaches to Single-Cell Analysis
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Novel Design of Luminescent Sensors and Optical Switches for Single Cell Analysis.2018

    • Author(s)
      Takeaki Ozawa
    • Organizer
      4th International Symposium on Molecular Imaging and Nanomedicine
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Novel Optical Techniques to Explore the Functions of G Protein-coupled Receptors (GPCRs)2018

    • Author(s)
      Takeaki Ozawa
    • Organizer
      5th International Conference on Innovative Biology Medicine and Engineering (ICIBME)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Novel design of luminescent sensors and optical switches for cellular analysis-Opto-bioanalysis-2018

    • Author(s)
      Takeaki Ozawa
    • Organizer
      Hwasun Optical & Molecular Imaging Workshop and Symposium (HOWS)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Imaging and Manipulating GPCRs in Live Cells with External Light.2018

    • Author(s)
      Takeaki Ozawa
    • Organizer
      10th Singapore International Chemistry Conference (SICC10)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2018-07-25   Modified: 2023-01-30  

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