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Development of a circular dichroism measuring system with high sensitivity and high time-resolution and its application for creation of biofunctional materials

Research Project

Project/Area Number 18K19102
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 35:Polymers, organic materials, and related fields
Research InstitutionTohoku University

Principal Investigator

Wada Takehiko  東北大学, 多元物質科学研究所, 教授 (20220957)

Project Period (FY) 2018-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords円二色性 / 時間分解 / 楕円偏光 / 高感度 / CDスペクトル / 二重らせんDNA / ポルフィリン誘導体 / インターカレーション / 円偏光二色性 / 高感度測定 / タンパク質 / 核酸 / 高次構造
Outline of Final Research Achievements

The detection of the complex formation process between biomolecules have received much attention not only basic scientific interest but also the development of biofunctional materials. Direct observation of the dynamic behavior of the complex formation process is considered to be important for highly efficient and accurate molecular development. Conventionally, various time-resolved spectral measurements have been applied to direct observation of dynamic behavior, but circular dichroism (CD) spectral measurement can detect the dynamic behavior of structural changes with high sensitivity. How to improve the low S/N originated from small CD peak intensities drawbacks of the CD measurement system was pointed out as a serious problem to be a general and powerful measurements tools. In this study, we have proposed and demonstrated the novel paradigm utilizing the ellipticall polarized light as detection of the CD measurement system with high sensitivity and high time resolution.

Academic Significance and Societal Importance of the Research Achievements

本研究で開発に成功した楕円偏光を用いるS/N向上と時間分解能の向上を達成した円二色性(CD)スペクトル測定法は、タンパク質や核酸と小分子間の複合体形成過程の動的挙動を高感度でかつ高時間分解能で観測可能で、基礎科学への、そして新規生体機能材料開発などへの貢献が期待され、その学術的意義は大きく、今後社会実装が望まれている。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (25 results)

All 2020 2019 2018 Other

All Int'l Joint Research (4 results) Journal Article (9 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 9 results,  Open Access: 7 results) Presentation (10 results) (of which Int'l Joint Research: 5 results,  Invited: 10 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] University of Bordeaux(フランス)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Case Western Reserve University(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Case Western Reserve University(米国)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] University de Bordeaux(フランス)

    • Related Report
      2018 Research-status Report
  • [Journal Article] Efficient Light‐Harvesting Antennae Resulting from the Dense Organization of Dyes into DNA Junctions though d ‐Threoninol2020

    • Author(s)
      Kashida Hiromu、Azuma Hidenori、Maruyama Ryoko、Araki Yasuyuki、Wada Takehiko、Asanuma Hiroyuki
    • Journal Title

      Angewandte Chemie International Edition

      Volume: - Issue: 28 Pages: 11360-11363

    • DOI

      10.1002/anie.202004221

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Underwater Bubble and Oil Repellency of Biomimetic Pincushion and Plastron-Like Honeycomb Films2020

    • Author(s)
      Chen Bihai、Wada Takehiko、Yabu Hiroshi
    • Journal Title

      Langmuir

      Volume: 36 Issue: 23 Pages: 6365-6369

    • DOI

      10.1021/acs.langmuir.0c00427

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Reversible Changes in the Orientation of Gold Nanorod Arrays on Polymer Brushes2020

    • Author(s)
      Sekizawa Yu、Mitomo Hideyuki、Nihei Mizuki、Nakamura Satoshi、Yonamine Yusuke、Kuzuya Akinori、Wada Takehiko、Ijiro Kuniharu
    • Journal Title

      Nanoscale Advances

      Volume: 2 Issue: 9 Pages: 3798-3803

    • DOI

      10.1039/d0na00315h

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Solvent Effects and Aggregation Phenomena Studied by Vibrational Optical Activity and Molecular Dynamics: The Case of Pantolactone2020

    • Author(s)
      Ghidinelli Simone、Abbate Sergio、Koshoubu Jun、Araki Yasuyuki、Wada Takehiko、Longhi Giovanna
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 124 Issue: 22 Pages: 4512-4526

    • DOI

      10.1021/acs.jpcb.0c01483

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 1.Development of an Engineered Photoactive Yellow Protein as a Cross-Linking Junction for Construction of Photoresponsive Protein-Polymer Conjugates2019

    • Author(s)
      Ui, Mihoko; Miyauchi, Yusuke; Inoue, Masataka; Murakami, Makoto; Araki, Yasuyuki; Wada, Takehiko; Kinbara, Kazushi
    • Journal Title

      ChemPhotoChem

      Volume: 3 Issue: 6 Pages: 356-360

    • DOI

      10.1002/cptc.201900024

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] 2.N-Benzoyl-Protected Peptide Nucleic Acid (PNA) Monomers Expand the Range of Nucleobases Available for PNA-DNA Chimera2019

    • Author(s)
      ito Inagaki , Ryohei Uematsu , Tatsuya Mizutani , Daisuke Unabara, Yasuyuki Araki , Seiji Sakamoto , Hiromu Kashida , Masaki Nishijima , Hiroyuki Asanuma , Yoshihisa Inoue , and Takehiko Wada
    • Journal Title

      Chemistry Letters

      Volume: 48 Issue: 4 Pages: 341-344

    • DOI

      10.1246/cl.181048

    • NAID

      130007624438

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 3.Transient Circular Dichroism Measurement of The Excited Triplet State of Pristine Hexahelicene in Solution at Room Temperature2019

    • Author(s)
      Makoto Kuronuma , Takehito Sato , Yasuyuki Araki ,* Tadashi Mori , Seiji Sakamoto, Yoshihisa Inoue , Osamu Ito , and Takehiko Wada
    • Journal Title

      Chemistry Letters

      Volume: 48 Issue: 4 Pages: 357-360

    • DOI

      10.1246/cl.190012

    • NAID

      130007624434

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Quantitative evaluation of energy migration between identical chromophores enabled by breaking symmetry2018

    • Author(s)
      Hiromu Kashida, Hayato Kawai, Ryoko Maruyama, Yuta Kokubo, Yasuyuki Araki, Takehiko Wada, Hiroyuki Asanuma
    • Journal Title

      Communications Chemistry (Nature)

      Volume: 1 Issue: 1 Pages: 91-98

    • DOI

      10.1038/s42004-018-0093-0

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Concentration-dependent photophysical switching in mixed self-assembled monolayers of pentacene and perylenediimide on gold nanoclusters2018

    • Author(s)
      D. Kato, H. Sakai, Y. Araki, T. Wada, N. V. Tkachenko, T. Hasobe
    • Journal Title

      Phys. Chem. Chem. Phys.

      Volume: 20 Issue: 13 Pages: 8695-8706

    • DOI

      10.1039/c8cp00174j

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 細胞内環境応答型人工核酸システムの開発 ‐ヌクレアーゼ活性制御機能を有する癌細胞選択的核酸医薬創製を目指して‐2020

    • Author(s)
      和田健彦
    • Organizer
      長崎大学大学院医歯薬学総合研究科 第3回機能性分子化学フォーラム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Development of high sensitive and high time-resolve circular dichroism (CD) measurement system2019

    • Author(s)
      Takehiko WADA
    • Organizer
      Advances in Noncanonical Nucleic Acids 2019 (ANNA2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Synthesis of Intracellular Condition Responsive Artificial Nucleic Acids for the Catalytic Oligonucleotide Therapeutics2019

    • Author(s)
      Takehiko WADA
    • Organizer
      6th Annual Joint Data Science in Life Science and Engineering Symposium
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 弱い相互作用を協働的に活用した不斉光反応制御 ‐生体高分子をキラル反応場として活用した超分子不斉光反応系を中心として‐2019

    • Author(s)
      和田健彦
    • Organizer
      第40回光化学若手の会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 天然ヌクレアーゼ活性超越に資するDNA-人工核酸キメラ分子構造設計と機能評価2019

    • Author(s)
      和田健彦
    • Organizer
      第68回高分子討論会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Development of Cancer Cell-Specific Oligonucleotide Therapeutics Delivery System with MMP Activatable PEG-Conjugated Oligoarginine Peptides2018

    • Author(s)
      Takehiko WADA
    • Organizer
      FIBER International Summit for Nucleic Acids 2018 (FISNA-2018)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Supramolecular Asymmetric Photochirogenesis (SMAP) of 2-Anthracene Carboxylate and  2,6-Anthracene Dicarboxylate mediated by Serum Albumin as Chiral Reaction Nanoreactor - Utilizing a Dynamics of Photoexcited Molecule for New Type of Asymmetric Photoreaction -2018

    • Author(s)
      Takehiko WADA
    • Organizer
      Special Lecture of University de Bordeaux.
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Supramolecular Asymmetric Photochirogenesis (SMAP) of 2-Anthracene Carboxylate mediated by Serum Albumin as Chiral Reaction Nanoreactor2018

    • Author(s)
      Takehiko WADA
    • Organizer
      Special Lecture of Technische University Munchen.
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 細胞内環境応答型人工核酸の開発 -イスキミア(虚血)細胞選択的核酸医薬の創製を目指して-2018

    • Author(s)
      和田健彦
    • Organizer
      東京医科歯科大学・脳統合機能研究センター講演会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] タンパク質キラルキャビティーを活用した新規分子結合制御法の創成2018

    • Author(s)
      和田健彦
    • Organizer
      第67回高分子討論会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Book] 刺激応答性高分子ハンドブック2018

    • Author(s)
      宮田隆志監修;和田健彦、片岡一則、石原一彦、浅沼浩之、伊藤耕三、中條善樹、秋吉一成、原田明、上垣外正己、大塚英幸、浜地格、有賀克彦、丸山厚、大矢裕一、古川英光、佐藤尚弘、柴山充弘、田中敬二、前田瑞夫、他
    • Total Pages
      864
    • Publisher
      (株)エヌ・ティー・エス
    • Related Report
      2018 Research-status Report
  • [Patent(Industrial Property Rights)] ・ キメラ分子、医薬組成物、標的核酸の切断方法、及び標的核酸切断用又は診断用キット2019

    • Inventor(s)
      和田健彦
    • Industrial Property Rights Holder
      和田健彦
    • Industrial Property Rights Type
      特許
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report

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Published: 2018-07-25   Modified: 2021-02-19  

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