Development of screening method for lipid raft inhibitors
| Project/Area Number |
18K19149
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 37:Biomolecular chemistry and related fields
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2018-06-29 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
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| Keywords | 脂質ラフト / FRET / スフィンゴミエリン / スフィンゴミエリ / 阻害剤 / 蛍光 |
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| Outline of Final Research Achievements |
Cell membranes have micro-domains called lipid rafts, but their physiological significance has yet to be clarified. On the other hand, enzyme inhibitors have been used not only as drugs but also as molecular probes for elucidating enzyme functions. Therefore, if a “raft inhibitor” that inhibits raft formation is available, it can be used as a molecular probe for exploring the function and properties of lipid rafts. In this study, we succeeded in developing an assay method for "raft inhibitors" by utilizing fluorescent lipids that selectively accumulate in raft or non-raft domains and observing the energy transfer between them. We also made this assay possible with artificial cell membranes and biological membranes, and succeeded in searching for new candidate compounds for raft inhibitors.
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| Academic Significance and Societal Importance of the Research Achievements |
脂質ラフトは、各種タンパク質が特異的に集積することでシグナル伝達の“足場”として機能すると考えられているが、その生理的役割はまだ完全には解明されていない。そこで、本研究によりラフト阻害剤を分子プローブとしてラフト研究に供することができれば、ラフト形成の生理的意義をより明確にできると期待される。
また、脂質ラフトが生理的に重要な働きをしているとすると、ラフトを破壊する薬剤、すなわちラフト阻害剤は何らかの毒性や生理作用を示すはずである。したがって、本研究で開発したアッセイ法は、創薬における副作用スクリーニングとしても機能すると期待される。
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Report
(3 results)
Research Products
(44 results)
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[Journal Article] Defining raft domains in the plasma membrane2019
Author(s)
Akihiro Kusumi、Takahiro K. Fujiwara、Taka A. Tsunoyama、Rinshi S. Kasai、An‐An Liu、Koichiro M. Hirosawa、Masanao Kinoshita、Nobuaki Matsumori、Naoko Komura、Hiromune Ando、Kenichi G. N. Suzuki
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Journal Title
Traffic
Volume: 21
Issue: 1
Pages: 106-137
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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