| Project/Area Number |
18K19159
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 38:Agricultural chemistry and related fields
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2018-06-29 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | L系列糖質 / オリゴ糖 / L-グリコシダーゼ / L-オリゴ糖 / 基質特異性 / L-グルコシド加水分解酵素 |
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| Outline of Final Research Achievements |
This study was conducted on the basis that most of the natural carbohydrates are composed of the D-series, but I believe that even if L-series monosaccharides and their oligosaccharides are naturally occuring, they are not theoretically strange. We discovered a new specificity for L-glycoside in an existing enzyme, and succeeded in obtaining an enzyme with a novel L-glycoside specificity from a sequence database. In addition, these L-glycosidases were used to synthesize oligosaccharides consisting of L-series carbohydrates and a mixture of D- and L-series oligosaccharides.
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| Academic Significance and Societal Importance of the Research Achievements |
天然に新規L-グリコシダーゼを発見した本研究は，既存のL-フコシドやL-ラムノシドに加えて新しいL-グリコシドが天然に存在することを示唆するものである．新しい研究分野として今後発展させていきたいと考えている．
オリゴ糖や希少糖といわれる糖質には，腸内フローラを整えるなど，いわゆるプロビオティクス効果を有するものが数多く存在する．今回合成することに成功したL体糖質からなるオリゴ糖にもこれら効果が期待され，来る我が国の高齢化社会に向けて，あたらしい健康素材を提供する可能性を有していると考えている．
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